Root Bark of Paeonia suffruticosa Extract and Its Component Methyl Gallate Possess Peroxynitrite Scavenging Activity and Anti-Inflammatory Properties through NF-κB Inhibition in LPS-treated Mice
Abstract
:1. Introduction
2. Results
2.1. ONOO− Scavenging Activity of 70% EtOH Extracts from Korean Medicinal Plants
2.2. Effect of Root Bark of P. suffruticosa Extracts and Methyl Gallate on LPS-Induced Kidney Injury in Mice
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Korean Medicinal Plants
4.3. Preparation of Extracts
4.4. Fractionation of the 70% EtOH Extract of Root Bark of P. suffruticosa
4.5. HPLC Analysis of the EtOAc Fraction of Root Bark of P. suffruticosa
4.6. Assay of ONOO− Levels
4.7. Experimental Animals and Treatment
4.8. Measurement of ROS Levels in the Kidney
4.9. Preparation of Cytosol and Nuclear Fractions
4.10. Western Blotting
4.11. Statistical Analyses
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Herb Name | Family | Used Part |
---|---|---|
Lonicera japonica Thunb. | Caprifoliaceae | Whole |
Lysimachia christinae Hance | Primulaceae | Whole |
Scirpus yagara Ohwi | Cyperaceae | Root |
Paeonia suffruticosa Andrew | Paeoniaceae | Root bark |
Ailanthus altissima (Mill.) Swingle | Simaroubaceae | Leaf |
Pueraria thunbergiana Benth | Leguminosae/Fabaceae | Root |
Curcuma zedoaria (Christm.) Roscoe | Zingiberaceae | Root |
Extract | IC50 Value (µg/mL) a |
---|---|
Lonicera japonica Thunb. | 7.92 ± 0.72 |
Lysimachia christinae Hance | 13.19 ± 0.28 |
Scirpus yagara Ohwi | 11.74 ± 0.77 |
Paeonia suffruticosa Andrew | 7.69 ± 0.67 |
Ailanthus altissima (Mill.) Swingle | 12.64 ± 0.37 |
Pueraria thunbergiana Benth | 10.65 ± 0.04 |
Curcuma zedoaria (Christm.) Roscoe | 10.13 ± 0.14 |
l-Penicillamine b | 8.24 ± 0.24 |
Sample | IC50 Values (µg/mL) a |
---|---|
70% EtOH extract | 4.78 ± 0.13 |
CH2Cl2 fraction | 1.78 ± 0.05 |
EtOAc fraction | 0.25 ± 0.03 |
n-BuOH fraction | 4.19 ± 0.23 |
H2O fraction | 7.99 ± 0.79 |
l-Penicillamine b | 9.00 ± 0.38 |
Compound | Retention Time (min) | Calibration Equation a | Correlation Coefficient (r2) |
---|---|---|---|
Methyl gallate | 15.451 | y = 17.430x + 4.221 | 0.996 |
Sample | Content (mg/g) | RSD a (%) |
---|---|---|
70% EtOH extract | 5.07 ± 0.06 | 2.22 |
EtOAc fraction | 39.62 ± 1.04 | 4.53 |
Sample | IC50 Values (µM) a |
---|---|
Methyl gallate | 0.91 ± 0.26 |
l-Penicillamine b | 8.79 ± 0.17 |
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Park, D.J.; Jung, H.J.; Park, C.H.; Yokozawa, T.; Jeong, J.-C. Root Bark of Paeonia suffruticosa Extract and Its Component Methyl Gallate Possess Peroxynitrite Scavenging Activity and Anti-Inflammatory Properties through NF-κB Inhibition in LPS-treated Mice. Molecules 2019, 24, 3483. https://doi.org/10.3390/molecules24193483
Park DJ, Jung HJ, Park CH, Yokozawa T, Jeong J-C. Root Bark of Paeonia suffruticosa Extract and Its Component Methyl Gallate Possess Peroxynitrite Scavenging Activity and Anti-Inflammatory Properties through NF-κB Inhibition in LPS-treated Mice. Molecules. 2019; 24(19):3483. https://doi.org/10.3390/molecules24193483
Chicago/Turabian StylePark, Dong Jin, Hee Jin Jung, Chan Hum Park, Takako Yokozawa, and Ji-Cheon Jeong. 2019. "Root Bark of Paeonia suffruticosa Extract and Its Component Methyl Gallate Possess Peroxynitrite Scavenging Activity and Anti-Inflammatory Properties through NF-κB Inhibition in LPS-treated Mice" Molecules 24, no. 19: 3483. https://doi.org/10.3390/molecules24193483