28-Hydroxy-3-oxoolean-12-en-29-oic Acid, a Triterpene Acid from Celastrus Orbiculatus Extract, Inhibits the Migration and Invasion of Human Gastric Cancer Cells In Vitro
Abstract
:1. Introduction
2. Results
2.1. Effect of Oleanane-Type Triterpenoid Acid from Celastrus Orbiculatus Thunb. on the Viability of Human Gastric Cancer Cells
2.2. Effect of 28-Hydroxy-3-oxoolean-12-en-29-oic Acid on the Viability of Human Gastric Cancer Cells
2.3. 28-Hydroxy-3-oxoolean-12-en-29-oic Acid Prevents the Migration and Invasion of Gastric Cancer Cells
2.4. PerkinElmer Operetta CLS High-Content Imaging System Analysis
2.5. Effect of 28-Hydroxy-3-oxoolean-12-en-29-oic Acid on the Expression of Matrix Metalloproteinases (MMPs) in Human Gastric Cancer Cells
2.6. Effect of 28-Hydroxy-3-oxoolean-12-en-29-oic Acid on Expression of Epithelial–Mesenchymal Transition (EMT)-Related Proteins in Human Gastric Cells
2.7. Effect of 28-Hydroxy-3-oxoolean-12-en-29-oic Acid on the Expression of PI3K/Akt/Snail Signaling Proteins in Human Gastric Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Drug
4.2. Reagents
4.3. Cell Culture
4.4. Cell Viability Assay
4.5. Cell Invasion and Migration Assay
4.6. PerkinElmer Operetta CLS High-Content Imaging System Analysis
4.7. Western Blot Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chu, Z.; Wang, H.; Ni, T.; Tao, L.; Xiang, L.; Zhou, Z.; Qian, Y.; Sunagawa, M.; Liu, Y. 28-Hydroxy-3-oxoolean-12-en-29-oic Acid, a Triterpene Acid from Celastrus Orbiculatus Extract, Inhibits the Migration and Invasion of Human Gastric Cancer Cells In Vitro. Molecules 2019, 24, 3513. https://doi.org/10.3390/molecules24193513
Chu Z, Wang H, Ni T, Tao L, Xiang L, Zhou Z, Qian Y, Sunagawa M, Liu Y. 28-Hydroxy-3-oxoolean-12-en-29-oic Acid, a Triterpene Acid from Celastrus Orbiculatus Extract, Inhibits the Migration and Invasion of Human Gastric Cancer Cells In Vitro. Molecules. 2019; 24(19):3513. https://doi.org/10.3390/molecules24193513
Chicago/Turabian StyleChu, Zewen, Haibo Wang, Tengyang Ni, Li Tao, Liangliang Xiang, Zhen Zhou, Yayun Qian, Masataka Sunagawa, and Yanqing Liu. 2019. "28-Hydroxy-3-oxoolean-12-en-29-oic Acid, a Triterpene Acid from Celastrus Orbiculatus Extract, Inhibits the Migration and Invasion of Human Gastric Cancer Cells In Vitro" Molecules 24, no. 19: 3513. https://doi.org/10.3390/molecules24193513