Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analysing the Components of Chrysanthemum in Weakly Basic Conditions
2.2. Retention Rate of 3-CQA and A7G under Basic Conditions
2.3. HPLC and UV Analysis
2.4. UPLC-Q-TOF-MS Analysis
3. Materials and Methods
3.1. Materials
3.2. Sample Preparation
3.3. Extraction with Ultrasound Method
3.4. Separation of the Components under Weakly Basic Conditions by HPLC
3.5. Hydrolysis Behaviour of 3-CQA and A7G under Basic Conditions.
3.6. Analysis of the Hydrolysates of 3-CQA and A7G with HPLC and UV
3.7. UPLC-Q-TOF-MS Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds chlorogenic acid and apigenin-7-O-glucoside are available from the authors. |
Samples | Codes | Company | Origins |
---|---|---|---|
C. morifolium ‘Gongju’ | GJ | Beijing Tongrentang Health Pharmaceutical Industry Co., Ltd. | Huangshan City, Anhui Province |
C. morifolium ‘Hangbaiju’ | HBJ | Beijing Tongrentang Health Pharmaceutical Industry Co., Ltd. | Tongxiang City, Zhejiang Province |
C. morifolium ‘Taiju’ | TJ | Beijing Zhang Yiyuan Jinqiao Tea Co., Ltd. | Zhongwei City, Ningxia Hui Autonomous Region |
C. morifolium ‘Boju’ | BJ | Bozhou Zhongyitang Chinese Medicinal Materials Sales Co., Ltd. | Bozhou City, Anhui Province |
C. morifolium ‘Chuju’ | CJ | Anhui Jutai Chuju Herb Science and Technology Co., Ltd. | Chuzhou City, Anhui Province |
C. morifolium ‘Huangju’ | HJ | Huangshan Dingxiangwu Ecological Agriculture Development Co., Ltd. | Shangrao City, Jiangxi Province |
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Wang, Y.; Xu, Z.; Wu, Y.; Li, M.; Pang, S.; Liang, Z.; Ni, Y. Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea. Molecules 2019, 24, 297. https://doi.org/10.3390/molecules24020297
Wang Y, Xu Z, Wu Y, Li M, Pang S, Liang Z, Ni Y. Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea. Molecules. 2019; 24(2):297. https://doi.org/10.3390/molecules24020297
Chicago/Turabian StyleWang, Yuxiao, Zhenzhen Xu, Yue Wu, Mo Li, Sicheng Pang, Zhe Liang, and Yuanying Ni. 2019. "Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea" Molecules 24, no. 2: 297. https://doi.org/10.3390/molecules24020297