Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of HPLC Analytical Conditions
2.2. Co-Extraction of Acrylamide and 5-HMF
2.3. Co-Purification of Acrylamide and 5-HMF by SPE
2.4. Application and Evaluation of the Established Method in Thermally Processed Foods
3. Materials and Methods
3.1. Reagents and Materials
3.2. Standards and Solutions Preparation
3.3. Instruments
3.4. Extraction of Acrylamide and 5-hydroxymethyl Furfural
3.5. Purification and Enrichment of Acrylamide and 5-HMF
3.6. Method Recovery in Food Sample
3.7. HPLC-DAD Analysis
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Standard | Linear Equation | R2 | Linear Range (μg/L) | LOD (μg/L) | LOQ (μg/L) |
---|---|---|---|---|---|
Acrylamide | Y = 100661.2X + 2159.18 | 0.9999 | 25–5000 | 6.90 | 20.90 |
5-HMF | Y = 95429.02X − 817.05 | 0.9999 | 25–5000 | 4.66 | 14.12 |
Sample. | Acrylamide | 5-HMF | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Original Concn. (μg/kg) | Spiked Concn. (μg/kg) | Detected Concn. (μg/kg) | Recovery (%) | RSD (%) | Original Concn. (μg/kg) | Spiked Concn. (μg/kg) | Detected Concn.(μg/kg) | Recovery (%) | RSD (%) | |
Biscuits | 378.6 ± 4.38 | 100 | 467.4 ± 6.75 | 88.84 | 1.44 | 739.75 ± 14.89 | 100 | 829.4 ± 8.36 | 89.67 | 1.01 |
200 | 562.8 ± 9.61 | 92.14 | 1.71 | 500 | 1220.6 ± 15.1 | 96.17 | 1.24 | |||
500 | 862.1 ± 6.73 | 96.70 | 0.78 | 1000 | 1686.9 ± 18.2 | 94.72 | 1.08 | |||
Bread | 186.2 ± 5.40 | 100 | 275.8 ± 3.84 | 89.69 | 1.39 | 1885.67 ± 16.52 | 100 | 1971.4 ± 15.8 | 85.68 | 0.80 |
200 | 375.9 ± 5.81 | 94.87 | 1.55 | 500 | 2351.8 ± 12.9 | 93.22 | 0.55 | |||
500 | 666.3 ± 9.91 | 96.03 | 1.49 | 1000 | 2813.3 ± 18.6 | 92.76 | 0.66 | |||
Chinese Doughnut | 173.3 ± 7.11 | 100 | 264.1 ± 8.86 | 90.78 | 3.35 | 419.42 ± 14.25 | 100 | 507.6 ± 6.88 | 88.22 | 1.36 |
200 | 348.8 ± 7.22 | 87.72 | 2.07 | 500 | 894.6 ± 7.04 | 95.03 | 0.79 | |||
500 | 633.2 ± 6.88 | 91.97 | 1.09 | 1000 | 1359.8 ± 9.92 | 94.04 | 0.73 | |||
Breakfast Cereals | 284.8 ± 4.11 | 100 | 376.1 ± 5.09 | 91.29 | 1.35 | 1166.50 ± 14.51 | 100 | 1253.3 ± 10.3 | 86.75 | 0.82 |
200 | 473.8 ± 3.73 | 94.48 | 0.79 | 500 | 1626.8 ± 11.6 | 92.07 | 0.71 | |||
500 | 762.5 ± 6.92 | 95.54 | 0.91 | 1000 | 2109.8 ± 12.7 | 94.33 | 0.60 | |||
Milk-Based Baby Food | 100.2 ± 0.69 | 100 | 192.5 ± 5.07 | 92.31 | 2.63 | 194.58 ± 5.28 | 100 | 287.9 ± 8.10 | 93.35 | 2.81 |
200 | 290.1 ± 3.16 | 94.92 | 1.09 | 500 | 648.6 ± 8.61 | 90.80 | 1.33 | |||
500 | 579.1 ± 8.17 | 95.77 | 1.41 | 1000 | 1114.4 ± 13.2 | 91.98 | 1.18 |
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Shi, J.; Shao, Z.; Li, H.; Zhang, Y.; Wang, S. Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods. Molecules 2019, 24, 3734. https://doi.org/10.3390/molecules24203734
Shi J, Shao Z, Li H, Zhang Y, Wang S. Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods. Molecules. 2019; 24(20):3734. https://doi.org/10.3390/molecules24203734
Chicago/Turabian StyleShi, Jiaqi, Zeping Shao, Honglei Li, Yan Zhang, and Shuo Wang. 2019. "Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods" Molecules 24, no. 20: 3734. https://doi.org/10.3390/molecules24203734