A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells
Abstract
:1. Introduction
2. Results
2.1. HSV-1 Replicates Effectively in HEK 293T Cells without Inducing IFN- Production
2.2. SILAC-Based Quantitative Proteomic Analysis of HSV-1 Infected HEK 293T Cells
2.3. Validation of SILAC-MS Data by Western Blot
2.4. Gene Ontology (GO) Analysis Reveals Biological Processes Regulated by HSV-1 Infection
2.5. Roles of IFITM3, CHCHD2, and TRIM27 on Virus Proliferation
3. Discussion
4. Materials and Methods
4.1. Cell and Virus
4.2. Subcellular Fractionation
4.3. Protein Extraction and Digestion
4.4. Liquid Chromatography/Mass Spectroscopy (LC-MS/MS) Analysis
4.5. Protein Identification and Quantification
4.6. Gene Ontology Analysis
4.7. Cell Transfection
4.8. Western Blot Analysis
4.9. Viral DNA Purification and Quantitationn
4.10. Intracellular RNA Extraction and Quantitation
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds (HSV-1) are available from the authors. |
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Wan, W.; Wang, L.; Chen, X.; Zhu, S.; Shang, W.; Xiao, G.; Zhang, L.-K. A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells. Molecules 2019, 24, 4215. https://doi.org/10.3390/molecules24234215
Wan W, Wang L, Chen X, Zhu S, Shang W, Xiao G, Zhang L-K. A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells. Molecules. 2019; 24(23):4215. https://doi.org/10.3390/molecules24234215
Chicago/Turabian StyleWan, Weiwei, Liangjie Wang, Xi Chen, Shenglin Zhu, Weijuan Shang, Gengfu Xiao, and Lei-Ke Zhang. 2019. "A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells" Molecules 24, no. 23: 4215. https://doi.org/10.3390/molecules24234215