Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. UV–Visible Spectroscopy
3.2. Fluorescence Spectroscopy
3.3. General Procedure for Multiwell DNAse I Assay
3.4. Lineweaver-Burk Plot
3.5. Detection of DNAse I in Human Serum
3.6. Determination of IC50 Values of DNAse I Inhibitors
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 1–4 are available from the authors. |
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Gabr, M.T.; Pigge, F.C. Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission. Molecules 2019, 24, 4390. https://doi.org/10.3390/molecules24234390
Gabr MT, Pigge FC. Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission. Molecules. 2019; 24(23):4390. https://doi.org/10.3390/molecules24234390
Chicago/Turabian StyleGabr, Moustafa T., and F. Christopher Pigge. 2019. "Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission" Molecules 24, no. 23: 4390. https://doi.org/10.3390/molecules24234390