The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction
Abstract
:1. Introduction
2. Results
2.1. The Virulence of Asp Induces Cell Damage in Human Primary Corneal Epithelial Cells
2.2. Biochemical Characterization of M28AP
2.3. The Role of M28Ap as Virulence Factor and Host Cell Damage
3. Discussion
3.1. Biochemical Characterization of M28AP
3.2. Cell Line Apoptosis by M28AP
4. Materials and Methods
4.1. Culture of Acanthamoeba Strains
4.2. Culture of C6 Glioma Rat Cell Line, A549 Human Cell Line and Human Primary Corneal Epithelial Cells
4.3. Isolation of Secreted Proteins
4.4. Cytopathic Effect Assay (CPE) and Giemsa Stain
4.5. Total RNA Isolation and cDNA Synthesis
4.6. DNA Construction, Cell Culture and Transient Transfection
4.7. SDS-PAGE and Coomassie Blue Staining
4.8. Biochemical Properties of Recombinant Protein M28AP
4.9. Ni-NTA-Atto Conjugates for Recombinant Protein M28AP- Cell Line Interaction
4.10. Annexin V Apoptosis Assay
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Huang, J.-M.; Chang, Y.-T.; Lin, W.-C. The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction. Molecules 2019, 24, 4573. https://doi.org/10.3390/molecules24244573
Huang J-M, Chang Y-T, Lin W-C. The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction. Molecules. 2019; 24(24):4573. https://doi.org/10.3390/molecules24244573
Chicago/Turabian StyleHuang, Jian-Ming, Yao-Tsung Chang, and Wei-Chen Lin. 2019. "The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction" Molecules 24, no. 24: 4573. https://doi.org/10.3390/molecules24244573