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Article

Mycosporine-Like Amino Acids (MAAs) in Time-Series of Lichen Specimens from Natural History Collections

by
Marylène Chollet-Krugler
1,
Thi Thu Tram Nguyen
1,2,
Aurelie Sauvager
1,
Holger Thüs
3,4,* and
Joël Boustie
1,*
1
CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR 6226, Univ Rennes, F-35000 Rennes, France
2
Department of Chemistry, Faculty of Science, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, An Khanh, Ninh Kieu, Can Tho, 902495 Vietnam
3
State Museum of Natural History Stuttgart, Rosenstein 1, 70191 Stuttgart, Germany
4
The Natural History Museum London, Cromwell Rd, Kensington, London SW7 5BD, UK
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(6), 1070; https://doi.org/10.3390/molecules24061070
Submission received: 12 February 2019 / Revised: 14 March 2019 / Accepted: 16 March 2019 / Published: 19 March 2019
(This article belongs to the Special Issue Lichens: Chemistry, Ecological and Biological Activities II)

Abstract

Mycosporine-like amino acids (MAAs) were quantified in fresh and preserved material of the chlorolichen Dermatocarpon luridum var. luridum (Verrucariaceae/Ascomycota). The analyzed samples represented a time-series of over 150 years. An HPLC coupled with a diode array detector (HPLC-DAD) in hydrophilic interaction liquid chromatography (HILIC) mode method was developed and validated for the quantitative determination of MAAs. We found evidence for substance specific differences in the quality of preservation of two MAAs (mycosporine glutamicol, mycosporine glutaminol) in Natural History Collections. We found no change in average mycosporine glutamicol concentrations over time. Mycosporine glutaminol concentrations instead decreased rapidly with no trace of this substance detectable in collections older than nine years. Our data predict that a screening for MAAs in organism samples from Natural History Collections can deliver results that are comparable to those obtained from fresh collections only for some MAAs (e.g., mycosporine glutamicol). For other MAAs, misleading, biased, or even false negative results will occur as a result of the storage sensitivity of substances such as mycosporine glutaminol. Our study demonstrates the value of pilot studies with time-series based on model taxa with a rich representation in the Natural History Collections.
Keywords: herbarium; fungarium; mycosporine-like amino acids; degradation; storage; Dermatocarpon luridum herbarium; fungarium; mycosporine-like amino acids; degradation; storage; Dermatocarpon luridum

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MDPI and ACS Style

Chollet-Krugler, M.; Nguyen, T.T.T.; Sauvager, A.; Thüs, H.; Boustie, J. Mycosporine-Like Amino Acids (MAAs) in Time-Series of Lichen Specimens from Natural History Collections. Molecules 2019, 24, 1070. https://doi.org/10.3390/molecules24061070

AMA Style

Chollet-Krugler M, Nguyen TTT, Sauvager A, Thüs H, Boustie J. Mycosporine-Like Amino Acids (MAAs) in Time-Series of Lichen Specimens from Natural History Collections. Molecules. 2019; 24(6):1070. https://doi.org/10.3390/molecules24061070

Chicago/Turabian Style

Chollet-Krugler, Marylène, Thi Thu Tram Nguyen, Aurelie Sauvager, Holger Thüs, and Joël Boustie. 2019. "Mycosporine-Like Amino Acids (MAAs) in Time-Series of Lichen Specimens from Natural History Collections" Molecules 24, no. 6: 1070. https://doi.org/10.3390/molecules24061070

APA Style

Chollet-Krugler, M., Nguyen, T. T. T., Sauvager, A., Thüs, H., & Boustie, J. (2019). Mycosporine-Like Amino Acids (MAAs) in Time-Series of Lichen Specimens from Natural History Collections. Molecules, 24(6), 1070. https://doi.org/10.3390/molecules24061070

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