Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry
Abstract
:1. Introduction
2. Materials and methods
2.1. Chemicals and Reagents
2.2. Instrumentation
2.3. Preparation and Characterization of GO-Tabs
2.4. Preparation of Stock, Standard and Quality Control Solutions
2.5. Sample Preparation
3. Results and Discussion
3.1. GO-Tab Morphology Analysis
3.2. Optimization of Extraction Protocol
3.2.1. Extraction Time
3.2.2. Desorption Time
3.2.3. Type of Desorption Solvent
3.2.4. Effect of pH
3.3. Adsorption Capacity
3.4. Selectivity of the GO-Tabs
3.5. Method Validation
3.5.1. Calibration, Selectivity and Extraction Efficiency
3.5.2. Accuracy and Precision
3.5.3. Method Selectivity and Matrix Effects
3.5.4. Carry-Over and Reuse of GO-Tabs
4. Analysis of Patient Samples
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Compound | Sample (Conc.) | Accuracy (%) (n = 18) | Precision (RSD%) | |
---|---|---|---|---|
Intra-day (n = 6) | Inter-day (n = 18) | |||
Omeprazole | QCL (6.0 nM) | 105.5 | 5.4 | 7.8 |
QCM (800 nM) | 106.4 | 5.1 | 7.5 | |
QCH (1600 nM) | 87.8 | 4.1 | 5.6 |
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Zohdi, Z.; Hashemi, M.; Uheida, A.; Moein, M.M.; Abdel-Rehim, M. Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry. Molecules 2019, 24, 1191. https://doi.org/10.3390/molecules24071191
Zohdi Z, Hashemi M, Uheida A, Moein MM, Abdel-Rehim M. Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry. Molecules. 2019; 24(7):1191. https://doi.org/10.3390/molecules24071191
Chicago/Turabian StyleZohdi, Zeynab, Mahdi Hashemi, Abdusalam Uheida, Mohammad Mahdi Moein, and Mohamed Abdel-Rehim. 2019. "Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry" Molecules 24, no. 7: 1191. https://doi.org/10.3390/molecules24071191