Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay
Abstract
:1. Introduction
2. Results and Discussion
2.1. Verification of Hapten
2.2. Optimization of the Bic-ELCIA
2.3. Analytical Bic-ELISA for Acetamiprid
2.4. Cross-Reactivity
2.5. Accuracy
2.6. Analysis of Authentic Samples
3. Materials and Methods
3.1. Reagents
3.2. Instruments and Equipment
3.3. Preparation of Coating Antigens
3.4. Biotinylation of Anti-Acetamiprid mAb
3.5. Performance of Biotinylated Indirect Competitive Enzyme-Linked immunosorbent Assay (Bic-ELISA)
3.6. Immunoassay Optimization
3.7. Cross-Reactivity
3.8. Recovery
3.9. Real Sample
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Compound | Chemical Structure | IC50 (ng/mL) | CR (%) |
---|---|---|---|
Acetamiprid | | 1.7 | 100 |
Thiacloprid | | 102.6 | 1.66 |
Thiamethoxam | | >400 | <0.5 |
Imidacloprid | | >400 | <0.5 |
Dinotefuran | | >400 | <0.5 |
Nitenpyram | | >400 | <0.5 |
Clothianidin | | >400 | <0.5 |
2,4-D | | >400 | <0.5 |
Sample | Spiked (ng/g) | Intra-Assay (n = 3) | Inter-Assay (n = 3) | ||||
---|---|---|---|---|---|---|---|
Mean ± SD a (ng/g) | Average Recovery (%) | RSD b (%) | Mean ± SD (ng/g) | Average Recovery (%) | RSD (%) | ||
Camellia pollen | 10 | 10.8 ± 1.0 | 108.0 | 9.3 | 11.1 ± 1.3 | 110.9 | 11.7 |
20 | 18.7 ± 1.4 | 93.5 | 7.7 | 18.1 ± 1.3 | 90.5 | 7.2 | |
50 | 46.0 ± 2.7 | 92.0 | 5.9 | 44.7 ± 3.3 | 89.4 | 7.4 | |
Lotus pollen | 10 | 10.8 ± 1.0 | 107.6 | 9.0 | 10.6 ± 1.1 | 105.8 | 10.4 |
20 | 16.2 ± 1.8 | 81.1 | 10.9 | 17.3 ± 1.2 | 86.7 | 6.9 | |
50 | 44.0 ± 2.1 | 88.0 | 4.8 | 42.7 ± 2.7 | 85.4 | 6.3 | |
Rape pollen | 10 | 10.4 ± 1.1 | 104.2 | 10.6 | 10.5 ± 0.9 | 104.9 | 8.6 |
20 | 21.1 ± 1.8 | 105.5 | 8.3 | 20.3 ± 1.6 | 101.4 | 7.9 | |
50 | 52.6 ± 2.7 | 105.2 | 5.2 | 50.7 ± 3.1 | 101.3 | 6.1 |
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Fang, Q.; Zu, Q.; Hua, X.; Lv, P.; Lin, W.; Zhou, D.; Xu, Z.; Fan, J.; Li, X.; Cao, H. Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay. Molecules 2019, 24, 1265. https://doi.org/10.3390/molecules24071265
Fang Q, Zu Q, Hua X, Lv P, Lin W, Zhou D, Xu Z, Fan J, Li X, Cao H. Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay. Molecules. 2019; 24(7):1265. https://doi.org/10.3390/molecules24071265
Chicago/Turabian StyleFang, Qingkui, Quan Zu, Xiude Hua, Pei Lv, Wanwen Lin, Dahe Zhou, Zihan Xu, Jiarui Fan, Xiaohan Li, and Haiqun Cao. 2019. "Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay" Molecules 24, no. 7: 1265. https://doi.org/10.3390/molecules24071265