The Comprehensive Roles of ATRANORIN, A Secondary Metabolite from the Antarctic Lichen Stereocaulon caespitosum, in HCC Tumorigenesis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Atranorin Inhibits HCC Cell Growth
2.2. Cell Cycle in HCC is Attenuated by Atranorin Treatment
2.3. Atranorin Enhances Cell Death
2.4. Atranorin Enhances Metastatic Potential of HCC
3. Materials and Methods
3.1. Lichen Material
3.2. Extraction and Isolation
3.3. Cell Culture and Maintenance
3.4. Cell Proliferation Assay
3.5. Cell Cycle Assay
3.6. Cell Death Determination Using FACS
3.7. Wound-Healing Assay
3.8. Migration and Invasion Assay
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Sample of the compound (Atranorin) is available from the authors. |
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Jeon, Y.-J.; Kim, S.; Kim, J.H.; Youn, U.J.; Suh, S.-S. The Comprehensive Roles of ATRANORIN, A Secondary Metabolite from the Antarctic Lichen Stereocaulon caespitosum, in HCC Tumorigenesis. Molecules 2019, 24, 1414. https://doi.org/10.3390/molecules24071414
Jeon Y-J, Kim S, Kim JH, Youn UJ, Suh S-S. The Comprehensive Roles of ATRANORIN, A Secondary Metabolite from the Antarctic Lichen Stereocaulon caespitosum, in HCC Tumorigenesis. Molecules. 2019; 24(7):1414. https://doi.org/10.3390/molecules24071414
Chicago/Turabian StyleJeon, Young-Jun, Sanghee Kim, Ji Hee Kim, Ui Joung Youn, and Sung-Suk Suh. 2019. "The Comprehensive Roles of ATRANORIN, A Secondary Metabolite from the Antarctic Lichen Stereocaulon caespitosum, in HCC Tumorigenesis" Molecules 24, no. 7: 1414. https://doi.org/10.3390/molecules24071414