Glycomacropeptide Ameliorates Indomethacin-Induced Enteropathy in Rats by Modifying Intestinal Inflammation and Oxidative Stress
Abstract
:1. Introduction
2. Results
2.1. Effect of GMP on Food Intake and Body Weight Gain
2.2. Effect of GMP on Intestinal Damage, and Weight and Length
2.3. Effect of GMP on Hematological and Biochemical Parameters
2.4. Effect of GMP on Intestinal Inflammation
2.5. Effect of GMP on Intestinal Oxidative Damage
2.6. Effect of GMP on Mucosal Barrier Integrity
2.7. Effect of GMP on Survival Rate
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Indomethacin-Induced Enteropathy Model and Sample Collection
4.3. Changes in Food Intake and Body Weight
4.4. Macroscopic Assessment of Small Intestine Damage
4.5. Evaluation of Hematological and Biochemical Parameters
4.6. Myeloperoxidase Activity
4.7. Immunofluorescence Assay
4.8. Determination of Nitric oxide and Lipids Hydroperoxides
4.9. RNA Extraction, Retrotranscription and qPCR
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Not available. |
Target | Oligonucleotide | Accession Number (NCBI) |
---|---|---|
Claudin-1 | Fw: AACCTCTTACCCAACACCACG Rv: GCCAAGACCCTCATAGCCAT | NM_031699.2 |
Occludin | Fw: AGGACAGACCCAGACCACTA Rv: ACTCTTCGCTCTCCTCTCTG | NM_031329.2 |
MUC-2 | Fw: GTATGTGCTCGCCTGTATGC Rv: TGACCTCCAGATGTGAGCAG | XM_017604244.1 |
CXCL1 | Fw: GCACCCAAACCGAAGTCATAG Rv: TGTTGTCAGAAGCCAGCGTT | NM_030845.1 |
IL-1β | Fw: AAATCTCACAGCAGCATCTC Rv: ACTAGCAGGTCGTCATCATC | NM_031512.2 |
iNOS | Fw: GATGTGCTGCCTCTGGTCCT Rv: ACTCCAATCTCGGTGCCCAT | NM_012611.3 |
β-Actin | Fw: GTCGTACCACTGGCATTGTG Rv: GCTGTGGTGGTGAAGCTGTA | NM_031144.3 |
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Cervantes-García, D.; Bahena-Delgado, A.I.; Jiménez, M.; Córdova-Dávalos, L.E.; Ruiz-Esparza Palacios, V.; Sánchez-Alemán, E.; Martínez-Saldaña, M.C.; Salinas, E. Glycomacropeptide Ameliorates Indomethacin-Induced Enteropathy in Rats by Modifying Intestinal Inflammation and Oxidative Stress. Molecules 2020, 25, 2351. https://doi.org/10.3390/molecules25102351
Cervantes-García D, Bahena-Delgado AI, Jiménez M, Córdova-Dávalos LE, Ruiz-Esparza Palacios V, Sánchez-Alemán E, Martínez-Saldaña MC, Salinas E. Glycomacropeptide Ameliorates Indomethacin-Induced Enteropathy in Rats by Modifying Intestinal Inflammation and Oxidative Stress. Molecules. 2020; 25(10):2351. https://doi.org/10.3390/molecules25102351
Chicago/Turabian StyleCervantes-García, Daniel, Armida I. Bahena-Delgado, Mariela Jiménez, Laura E. Córdova-Dávalos, Vanessa Ruiz-Esparza Palacios, Esperanza Sánchez-Alemán, María C. Martínez-Saldaña, and Eva Salinas. 2020. "Glycomacropeptide Ameliorates Indomethacin-Induced Enteropathy in Rats by Modifying Intestinal Inflammation and Oxidative Stress" Molecules 25, no. 10: 2351. https://doi.org/10.3390/molecules25102351