Otoprotective Effect of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-Glucoside on Gentamicin-Induced Apoptosis in Mouse Cochlear UB/OC-2 Cells
Abstract
:1. Introduction
2. Results
2.1. Gentamicin Suppresses Cell Viability and Induces Cell Cytotoxicity in University of Bristol/Organ of Corti-2 (UB/OC-2) Cells
2.2. Gentamicin Promotes Apoptotic Cell Death in UB/OC-2 Cells
2.3. THSG Protects UB/OC-2 Cells against Gentamicin-Induced Oxidative Stress
2.4. THSG Impairs Gentamicin-Induced Mitochondrial-Dependent Apoptotic Pathway and Prevents Cell Death in UB/OC-2 Cells
3. Discussion
4. Materials and Methods
4.1. Cells and Materials
4.2. Cell Culture
4.3. Cell Viability Assay
4.4. LDH Cytotoxicity Assay
4.5. Western Blotting Analysis
4.6. ROS Detection Assay
4.7. Determination of SOD Activity
4.8. Hoechst 33258 Staining
4.9. JC-1 Staining
4.10. Cell Fractionation
4.11. Annexin V and PI Double Staining Assay
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Wen, Y.-H.; Lin, J.-N.; Wu, R.-S.; Yu, S.-H.; Hsu, C.-J.; Tseng, G.-F.; Wu, H.-P. Otoprotective Effect of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-Glucoside on Gentamicin-Induced Apoptosis in Mouse Cochlear UB/OC-2 Cells. Molecules 2020, 25, 3070. https://doi.org/10.3390/molecules25133070
Wen Y-H, Lin J-N, Wu R-S, Yu S-H, Hsu C-J, Tseng G-F, Wu H-P. Otoprotective Effect of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-Glucoside on Gentamicin-Induced Apoptosis in Mouse Cochlear UB/OC-2 Cells. Molecules. 2020; 25(13):3070. https://doi.org/10.3390/molecules25133070
Chicago/Turabian StyleWen, Yu-Hsuan, Jia-Ni Lin, Rong-Shuan Wu, Szu-Hui Yu, Chuan-Jen Hsu, Guo-Fang Tseng, and Hung-Pin Wu. 2020. "Otoprotective Effect of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-Glucoside on Gentamicin-Induced Apoptosis in Mouse Cochlear UB/OC-2 Cells" Molecules 25, no. 13: 3070. https://doi.org/10.3390/molecules25133070