Apoferritin and Apoferritin-Capped Metal Nanoparticles Inhibit Arginine Kinase of Trypanosoma brucei
Abstract
:1. Introduction
2. Results
2.1. Apoferritin and Apoferritin-Capped Nanoparticles Reduced the Activity of Arginine Kinase of T. brucei and Modulated Kinetic Parameters
2.2. Apoferritin and Apoferritin-Capped Nanoparticles Caused Significant Decreases in the Relative Activity of Arginine Kinase of T. brucei
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Determination of Protein Concentration and Activity of the Arginine Kinase of T. brucei
4.3. Assay to Determine Arginine Kinase Activity in the Presence of Apoferritin or Apoferritin-Nanoparticles
4.4. Data Presentation and Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors upon request. |
* Vmax (µmol/sec) | ** Vmax (µmol/sec) | Km (L-arginine) (mM) | Km (ATP) (mM) | * Mean Ki (nM) | ** Mean Ki (nM) | |
---|---|---|---|---|---|---|
Control Assay | 0.169 ± 0.004 | 0.170 ± 0.010 | 0.021 ± 0.01 | 0.005 ± 0.00 | - | - |
Apoferritin | 0.012 ± 0.001 | 0.016 ± 0.001 | 0.041 ± 0.00 | 0.015 ± 0.00 | 0.062 ± 0.01 | 0.001 ± 0.00 |
Apoferritin-AgNPs | 0.044 ± 0.002 | 0.034 ± 0.000 | 0.041 ± 0.01 | 0.011 ± 0.00 | 0.168 ± 0.03 | 0.057 ± 0.00 |
Apoferritin-AuNPs | 0.067 ± 0.006 | 0.016 ± 0.001 | 0.044 ± 0.00 | 0.015 ± 0.00 | 0.160 ± 0.00 | 0.003 ± 0.00 |
Apoferritin-PtNPs | 0.024 ± 0.007 | 0.030 ± 0.001 | 0.043 ± 0.00 | 0.001 ± 0.00 | 0.245 ± 0.03 | 0.002 ± 0.00 |
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Adeyemi, O.S.; Arowolo, A.T.; Hetta, H.F.; Al-Rejaie, S.; Rotimi, D.; Batiha, G.E.-S. Apoferritin and Apoferritin-Capped Metal Nanoparticles Inhibit Arginine Kinase of Trypanosoma brucei. Molecules 2020, 25, 3432. https://doi.org/10.3390/molecules25153432
Adeyemi OS, Arowolo AT, Hetta HF, Al-Rejaie S, Rotimi D, Batiha GE-S. Apoferritin and Apoferritin-Capped Metal Nanoparticles Inhibit Arginine Kinase of Trypanosoma brucei. Molecules. 2020; 25(15):3432. https://doi.org/10.3390/molecules25153432
Chicago/Turabian StyleAdeyemi, Oluyomi Stephen, Afolake T. Arowolo, Helal F. Hetta, Salim Al-Rejaie, Damilare Rotimi, and Gaber El-Saber Batiha. 2020. "Apoferritin and Apoferritin-Capped Metal Nanoparticles Inhibit Arginine Kinase of Trypanosoma brucei" Molecules 25, no. 15: 3432. https://doi.org/10.3390/molecules25153432