The Inhibitory Effects of Heterotrigona Itama Honey Marinades on the Formation of Carcinogenic Heterocyclic Amines in Grilled Beef Satay
Abstract
:1. Introduction
2. Results and Discussion
2.1. Marinade Uptake
2.2. Cooking Loss
2.3. Internal Temperature
2.4. LOD, LOQ, and Recovery of HCAs
2.5. Amino Acids, Creatinine, and Sugars Profile (Fructose, Glucose, Sucrose, Maltose)
2.6. HCAs Content in Grilled Beef Satay
2.7. The Influence of Types of Marinades
2.8. Reduction of HCAs
2.9. Multivariate Data Analysis
2.9.1. Orthogonal Partial Least Squares Regression
2.9.2. Partial Least Squares Regression
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. Raw Materials
3.3. Beef Satay Preparation
3.4. Grilling Conditions
3.5. Marinade Uptake
3.6. Cooking Loss
3.7. Analysis of HCAs
3.7.1. HCAs Extraction and Clean-Up Procedures
3.7.2. HPLC Analysis of HCAs
3.7.3. LOD, LOQ, and Recovery of HCAs
3.8. Determination of Precursors in Raw Beef Satay Samples
3.8.1. Amino Acids
3.8.2. Sugars Profile (Fructose, Glucose, Maltose, and Sucrose)
3.8.3. Creatinine
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Treatment | Marinade Uptake (%) | Cooking Loss (%) | Internal Temperature (°C) |
---|---|---|---|
Unmarinated | NA | 35.38 ± 0.34 a | 84.17 ± 0.15 a |
Marinated with: | |||
Table sugar (control) | 26.21 ± 0.18 c | 34.10 ± 0.25 b | 82.47 ± 0.47 b |
Apis mellifera honey | 29.78 ± 0.37 b | 32.69 ± 0.66 c | 80.83 ± 0.68 c |
Acacia honey | 33.32 ± 0.43 a | 28.88 ± 0.42 d | 75.40 ± 0.78 d |
Starfruit honey | 32.28 ± 0.57 a | 28.10 ± 0.29 d | 74.03 ± 0.57 d,e |
Gelam honey | 31.12 ± 0.76 a | 28.46 ± 0.39 d | 73.00 ± 0.20 e |
Amino Acids | Unmarinated Samples | Types of Marination | ||||
---|---|---|---|---|---|---|
Table Sugar | Apis Mellifera | Acacia | Starfruit | Gelam | ||
Alanine | 5.51 ± 0.33 a | 4.81 ± 0.13 b | 5.73 ± 0.22 a | 4.57 ± 0.17 b | 4.89 ± 0.60 b | 4.86 ± 0.12 b |
Sarcosine | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ |
Glycine | 1.96 ± 0.06 a | 1.54 ± 0.06 b | 1.77 ± 0.20 a | 1.24 ± 0.06 c | 1.31 ± 0.16 c | 1.43 ± 0.05 b,c |
α-Amino butyric acid | <LOQ c | <LOQ c | 0.19 ± 0.06 a | <LOQ c | 0.12 ± 0.00 b | 0.16 ± 0.07 a,b |
Valine | <LOQ d | 1.01 ± 0.09 b | 1.29 ± 0.03 a | 0.70 ± 0.07 c | <LOQ d | 0.80 ± 0.20 c |
β-Amino iso butyric acid | 2.40 ± 1.06 a | 1.77 ± 0.30 a | 1.99 ± 0.53 a | 1.53 ± 0.63 a | 1.94 ± 0.10 a | 1.73 ± 0.30 a |
Leucine | 1.58 ± 0.91 a | 1.59 ± 0.50 a | 1.37 ± 0.80 a,b | 1.85 ± 0.34 a | 0.37 ± 0.01 b | 1.40 ± 0.89 a,b |
Allo-isoleucine | 1.21 ± 0.70 a,b | 1.50 ± 1.01 a,b | 1.92 ± 1.27 a | <LOQ b | 2.65 ± 0.22 a | 1.31 ± 1.46 a,b |
Isoleucine | 0.17 ± 0.05 c | <LOQ d | 0.67 ± 0.11 a | 0.44 ± 0.03 b | <LOQ d | <LOQ d |
Threonine | <LOQ d | 0.71 ± 0.06 b | 0.89 ± 0.08 a | 0.50 ± 0.05 c | 0.44 ± 0.11 c | 0.67 ± 0.05 b |
Serine | 1.47 ± 0.25 d | 2.72 ± 0.10 a,b,c | 3.33 ± 0.22 a | 2.51 ± 0.01 b,c | 3.07 ± 0.29 a,b | 2.20 ± 0.92 c,d |
Proline | 0.33 ± 0.11 e | 1.44 ± 0.09 c | 2.30 ± 0.15 a | 1.18 ± 0.04 d | 1.29 ± 0.07 c,d | 1.66 ± 0.11 b |
Asparagine | <LOQ d | 1.56 ± 0.21 c | 2.42 ± 0.25 b | 1.48 ± 0.30 c | 2.06 ± 0.26 b | 2.94 ± 0.26 a |
Aspartic acid | 0.95 ± 0.06 b | 2.40 ± 1.50 a | 2.47 ± 0.25 a | 1.09 ± 0.02 b | 1.38 ± 0.71 a,b | |
Methionine | 1.32 ± 0.14 b | 1.61 ± 0.02 b | 1.64 ± 0.05 b | 1.43 ± 0.07 b | 2.17 ± 0.19 a | 1.52 ± 0.39 b |
3-hydroxyproline | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ |
Glutamic Acid | 1.60 ± 0.56 c | 4.50 ± 0.18 a | 5.09 ± 0.58 a | 3.20 ± 0.35 b | 3.52 ± 0.36 b | 3.42 ± 0.15 b |
Phenylalanine | 0.53 ± 0.13 d | 1.32 ± 0.05 c | 1.39 ± 0.14b c | 1.10 ± 0.04 c | 2.70 ± 0.34 a | 1.76 ± 0.34 b |
α-Amino adipic Acid | 1.03 ± 0.27 a | 0.68 ± 0.14 b | 0.72 ± 0.11 b | <LOQ d | 0.27 ± 0.08 c | 0.47 ± 0.14 b,c |
Glutamine | 4.28 ± 1.45 c | 5.63 ± 0.48 b,c | 8.01 ± 0.70 a | 6.63 ± 0.32 a,b | 7.26 ± 0.84 a | 7.95 ± 0.29 a |
Ornithine | 1.31 ± 0.05 b | 2.08 ± 0.22 a | 2.33 ± 0.33 a | 1.05 ± 0.03 b | 1.04 ± 0.22 b | 1.08 ± 0.0 b |
Lysine | 1.27 ± 0.05 | 2.02 ± 012 b | 2.09 ± 0.02 a,b | 2.17 ± 0.02 a,b | 2.26 ± 0.17 a | 2.24 ± 0.08 a |
Histidine | 1.64 ± 0.04 b,c | 1.82 ± 0.01 a,b,c | 1.92 ± 0.08 a | 1.75 ± 0.14 a,b,c | 1.62 ± 0.30 c | 1.89 ± 0.12 a,b |
Tyrosine | 0.70 ± 0.01 c | 0.82 ± 0.06 b,c | 0.69 ± 0.15 c | 0.94 ± 0.01 a,b | 0.81 ± 0.18 b,c | 1.03 ± 0.10 a |
Tryptophan | 0.98 ± 0.01 a | 1.07 ± 0.004 a | 1.07 ± 0.01 a | 1.03 ± 0.01 a | 1.12 ± 0.25 a | 1.05 ± 0.05 a |
Cysteine | ND | ND | ND | ND | ND | ND |
TOTAL | 30.05 ± 1.12 d | 41.17 ± 1.15 b | 51.22 ± 2.31 a | 38.18 ± 0.84 c | 42.01 ± 1.50 b | 42.96 ± 2.35 b |
Creatinine | 0.04 ± 0.004 | |||||
Fructose | ND e | 2.83 ± 0.01 d | 4.27 ± 0.12 b | 2.96 ± 0.04 c,d | 3.20 ± 0.01 c | 5.16 ± 0.40 a |
Glucose | 4.26 ± 0.03 b | 3.22 ± 0.02 c | 5.33 ± 0.32 a | 3.28 ± 0.04 c | 4.51 ± 0.06 b | 5.16 ± 0.12 a |
Sucrose | ND | ND | ND | ND | ND | ND |
Maltose | ND | ND | ND | ND | ND | ND |
Total sugar | 4.26 ± 0.03 e | 6.05 ± 0.01 d | 9.61 ± 0.44 b | 6.24 ± 0.07 d | 7.71 ± 0.06 c | 10.32 ± 0.52 a |
Treatments | Aminoimidazoazaarens (AIAs) | Amino Carbolines | |||||
---|---|---|---|---|---|---|---|
MeIQx | 4,8-DiMeIQx | PhIP | NH | H | AαC | Total HCAs | |
CTR | ND | ND | 1.22 ± 0.01 a | 141.64 ± 1.43 b | 6.27 ± 0.28 b | 0.63 ± 0.06 c | 149.76 ± 1.63 b |
TS | 5.25 ± 0.32 a | 8.23 ± 0.57 a | 0.18 ± 0.15 b | 314.00 ± 1.57 a | 14.99 ± 0.94 a | 1.97 ± 0.02 a | 344.62 ± 2.77 a |
AP | 1.29 ± 0.08 b | 5.93 ± 0.13 b | 0.06 ± 0.07 b,c | 134.16 ± 1.80 c | 3.98 ± 0.41 c | 1.57 ± 0.03 a | 147.42 ± 1.59 b |
AC | 1.78 ± 0.81 b | 3.72 ± 0.67 c | 0.07 ± 0.05 b,c | 40.60 ± 0.86 d | 2.43 ± 0.15 e | 0.84 ± 0.23 b | 49.44 ± 1.72 c |
SF | ND c | 4.00 ± 0.95 c | 0.01 ± 0.01 b,c | 31.55 ± 0.58 e | 3.69 ± 0.02 c,d | 0.53 ± 0.02 c | 39.79 ± 1.31 d |
GE | ND c | 1.68 ± 0.40 d | 0.06 ± 0.04 c | 11.93 ± 0.27 f | 3.00 ± 0.12 d,e | 0.08 ± 0.01 d | 16.75 ± 0.21 e |
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Shamsudin, S.; Selamat, J.; Sanny, M.; Jambari, N.N.; Sukor, R.; Praveena, S.M.; Khatib, A. The Inhibitory Effects of Heterotrigona Itama Honey Marinades on the Formation of Carcinogenic Heterocyclic Amines in Grilled Beef Satay. Molecules 2020, 25, 3874. https://doi.org/10.3390/molecules25173874
Shamsudin S, Selamat J, Sanny M, Jambari NN, Sukor R, Praveena SM, Khatib A. The Inhibitory Effects of Heterotrigona Itama Honey Marinades on the Formation of Carcinogenic Heterocyclic Amines in Grilled Beef Satay. Molecules. 2020; 25(17):3874. https://doi.org/10.3390/molecules25173874
Chicago/Turabian StyleShamsudin, Sharina, Jinap Selamat, Maimunah Sanny, Nuzul Noorahya Jambari, Rashidah Sukor, Sarva Mangala Praveena, and Alfi Khatib. 2020. "The Inhibitory Effects of Heterotrigona Itama Honey Marinades on the Formation of Carcinogenic Heterocyclic Amines in Grilled Beef Satay" Molecules 25, no. 17: 3874. https://doi.org/10.3390/molecules25173874