New Perspectives of S-Adenosylmethionine (SAMe) Applications to Attenuate Fatty Acid-Induced Steatosis and Oxidative Stress in Hepatic and Endothelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. SAMe Preparation
2.3. Cell Culture and Treatments
2.4. Protein Quantification
2.5. Quantification of Triglycerides
2.6. ROS Production and Lipid Peroxidation Determination
2.7. Oil-Red O Staining
2.8. Measurement of the Levels of Nitrites/Nitrates
2.9. Real-Time qPCR
2.10. Wound Healing Assay
2.11. Statistical Analysis
3. Results
3.1. Effects of SAMe on Lipid Accumulation in Hepatic Cells
3.2. Effects of SAMe on Oxidative Stress in Hepatic Cells
3.3. Effects of SAMe on Lipid Accumulation and Function in Endothelial Cells
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Vergani, L.; Baldini, F.; Khalil, M.; Voci, A.; Putignano, P.; Miraglia, N. New Perspectives of S-Adenosylmethionine (SAMe) Applications to Attenuate Fatty Acid-Induced Steatosis and Oxidative Stress in Hepatic and Endothelial Cells. Molecules 2020, 25, 4237. https://doi.org/10.3390/molecules25184237
Vergani L, Baldini F, Khalil M, Voci A, Putignano P, Miraglia N. New Perspectives of S-Adenosylmethionine (SAMe) Applications to Attenuate Fatty Acid-Induced Steatosis and Oxidative Stress in Hepatic and Endothelial Cells. Molecules. 2020; 25(18):4237. https://doi.org/10.3390/molecules25184237
Chicago/Turabian StyleVergani, Laura, Francesca Baldini, Mohamad Khalil, Adriana Voci, Pietro Putignano, and Niccolò Miraglia. 2020. "New Perspectives of S-Adenosylmethionine (SAMe) Applications to Attenuate Fatty Acid-Induced Steatosis and Oxidative Stress in Hepatic and Endothelial Cells" Molecules 25, no. 18: 4237. https://doi.org/10.3390/molecules25184237