Resveratrol Suppresses Cross-Talk between Colorectal Cancer Cells and Stromal Cells in Multicellular Tumor Microenvironment: A Bridge between In Vitro and In Vivo Tumor Microenvironment Study
Abstract
:1. Introduction
2. Results
2.1. Resveratrol Inhibits HCT116 Cell Proliferation Promoted by TNF-β- or Multicellular-TME, but Not by Sirt1-ASO
2.2. Resveratrol Inhibits HCT116 Cells Colonosphere Formation and Invasion Promoted by TNF-β- or Multicellular-TME, but Not by Sirt1-ASO
2.3. Resveratrol Suppresses Nuclear Translocation of p65-NF-κB in CRC Cells Stimulated by TNF-β- or Multicellular-TME, but Not by Sirt1-ASO
2.4. Resveratrol Modulates Suppression of Sirt1 Expression, NF-κB Phosphorylation and Biomarkers Linked with Inflammation, Invasion, Proliferation and Apoptosis in HCT116 Cells Induced by TNF-β- or Multicellular-TME, but Not by Sirt1-ASO
2.5. Resveratrol Suppresses Cancer Stem Cells in CRC Cell Populations Stimulated by TNF-β- or Multicellular-TME, but Not by Sirt1-ASO
2.6. Resveratrol Inhibits Intensive Crosstalk between HCT116 Cells and Stromal Cells Induced by TNF-β- or Multicellular-TME, but Not by Knockdown of Sirt1 with Sirt1-ASO
3. Discussion
4. Materials and Methods
4.1. Antibodies and Chemicals
4.2. Cell Lines and Cell Culture Conditions
4.3. Experimental Setup
4.4. Lipofectin Mediated Antisense Transfection
4.5. Alginate Culture, Colonosphere Formation and Invasion Assay
4.6. Cell Proliferation Assay
4.7. Immunofluorescence
4.8. Immunoblotting Assay
4.9. Statistical Evaluation
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Buhrmann, C.; Shayan, P.; Brockmueller, A.; Shakibaei, M. Resveratrol Suppresses Cross-Talk between Colorectal Cancer Cells and Stromal Cells in Multicellular Tumor Microenvironment: A Bridge between In Vitro and In Vivo Tumor Microenvironment Study. Molecules 2020, 25, 4292. https://doi.org/10.3390/molecules25184292
Buhrmann C, Shayan P, Brockmueller A, Shakibaei M. Resveratrol Suppresses Cross-Talk between Colorectal Cancer Cells and Stromal Cells in Multicellular Tumor Microenvironment: A Bridge between In Vitro and In Vivo Tumor Microenvironment Study. Molecules. 2020; 25(18):4292. https://doi.org/10.3390/molecules25184292
Chicago/Turabian StyleBuhrmann, Constanze, Parviz Shayan, Aranka Brockmueller, and Mehdi Shakibaei. 2020. "Resveratrol Suppresses Cross-Talk between Colorectal Cancer Cells and Stromal Cells in Multicellular Tumor Microenvironment: A Bridge between In Vitro and In Vivo Tumor Microenvironment Study" Molecules 25, no. 18: 4292. https://doi.org/10.3390/molecules25184292