An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of EGF–Cur-NLC
2.2. Crystallinity
2.3. In Vitro Release Study
2.4. Cell Proliferation and Migration Assays
2.5. Antioxidant Activity of EGF–Cur-NLC
2.6. In Vivo Wound Healing Study
3. Materials and Methods
3.1. Materials
3.2. Screening of Liquid Lipids and Surfactants
3.3. Preparation of EGF–Cur-NLC
3.4. Physicochemical Properties of EGF–Cur-NLC
3.5. Determination of Drug Content and EE
3.6. In Vitro Release Study of EGF–Cur-NLC
3.7. In Vitro Cell Study of EGF–Cur-NLC
3.7.1. Cell Culture Condition
3.7.2. Cell Proliferation Assay
3.7.3. Cell Migration Assay
3.8. In Vivo Study
3.8.1. Animals
3.8.2. Diabetic Wound Healing Animal Model
3.8.3. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the corresponding authors. |
(a) | ||||
Formulation | Precirol ATO 5 (mg) | Capryol 90 (mg) | EGF (μg) | Curcumin (mg) |
F1 | 70 | 30 | 100 | 1 |
F2 | 60 | 40 | 100 | 1 |
F3 | 50 | 50 | 100 | 1 |
(b) | ||||
Formulation | Particle Size (nm) | PDI | EE of EGF (%) | EE of Curcumin (%) |
F1 | 331.8 ± 29.1 | 0.31 ± 0.03 | 81.1 ± 0.8 | 99.4 ± 0.1 |
F2 | 394.3 ± 17.5 | 0.36 ± 0.01 | 79.0 ±1.9 | 99.2 ± 0.4 |
F3 | 509.2 ± 18.3 | 0.34 ± 0.02 | 79.8 ± 1.3 | 99.2 ± 0.0 |
Drug | n | k | R2 | Release Mechanism |
---|---|---|---|---|
EGF | 0.4 | 0.28 | 0.9 | Quasi-Fickian diffusion |
Cur | 0.37 | 0.4 | 0.89 | Quasi-Fickian diffusion |
EGF in EGF–Cur-NLC | 0.26 | 0.37 | 0.91 | Quasi-Fickian diffusion |
Cur in EGF–Cur-NLC | 0.31 | 0.31 | 0.85 | Quasi-Fickian diffusion |
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Lee, H.-J.; Jeong, M.; Na, Y.-G.; Kim, S.-J.; Lee, H.-K.; Cho, C.-W. An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats. Molecules 2020, 25, 4610. https://doi.org/10.3390/molecules25204610
Lee H-J, Jeong M, Na Y-G, Kim S-J, Lee H-K, Cho C-W. An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats. Molecules. 2020; 25(20):4610. https://doi.org/10.3390/molecules25204610
Chicago/Turabian StyleLee, Hye-Jin, Moses Jeong, Young-Guk Na, Sung-Jin Kim, Hong-Ki Lee, and Cheong-Weon Cho. 2020. "An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats" Molecules 25, no. 20: 4610. https://doi.org/10.3390/molecules25204610