Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity toward Neuro-2a Cells
2.2. DPPH Radical Scavenging Activity
2.3. Influence on PQ-Treated Neuro-2a Cell Viability
2.4. Influence on ROS Level and Mitochondrial Dysfunction in PQ-Treated Neuro-2a Cells
2.5. Influence on Hsp70 Level in PQ-Treated Neuro-2a Cells
2.6. Influence on Neurites Outgrowth in PQ-Treated Neuro-2a Cells
3. Discussion
4. Materials and Methods
4.1. Compounds
4.2. DPPH Radical Scavenging Assay
4.3. Neuro-2a Cell Culture
4.4. MTT Cell Viability Assay
4.5. FDA Cell Viability Assay
4.6. Propidium Iodide Straining
4.7. Paraquat-Induced Neurotoxicity
4.8. Reactive Oxygen Species (ROS) Level Analysis
4.9. Mitochondrial Membtane Potential (MMP) Detection
4.10. Hsp70 Level Analysis
4.11. Neurite Outgrowth Estimation
4.12. Data Evaluation
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Compound | Cytotoxicity (MTT Assay) | DPPH Radical Scavenging | |
---|---|---|---|
IC50, μM | % of Control at 10 μM | EC50, μM | |
1 | 85.7 ± 2.4 | 92.1 ± 7.1 | >100 |
2 | >100 | 96.9 ± 1.1 | >100 |
3 | >100 | 91.1 ± 4.1 | >100 |
4 | >100 | 95.3 ± 2.7 | >100 |
5 | >100 | 97.9 ± 3.3 | >100 |
Ascorbic acid | - | 20.5 ± 1.5 |
Sample Availability: Samples of the compounds are not available from the authors. | |
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Yurchenko, E.A.; Kolesnikova, S.A.; Lyakhova, E.G.; Menchinskaya, E.S.; Pislyagin, E.A.; Chingizova, E.A.; Aminin, D.L. Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity. Molecules 2020, 25, 5397. https://doi.org/10.3390/molecules25225397
Yurchenko EA, Kolesnikova SA, Lyakhova EG, Menchinskaya ES, Pislyagin EA, Chingizova EA, Aminin DL. Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity. Molecules. 2020; 25(22):5397. https://doi.org/10.3390/molecules25225397
Chicago/Turabian StyleYurchenko, Ekaterina A., Sophia A. Kolesnikova, Ekaterina G. Lyakhova, Ekaterina S. Menchinskaya, Evgeny A. Pislyagin, Ekaterina A. Chingizova, and Dmitry L. Aminin. 2020. "Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity" Molecules 25, no. 22: 5397. https://doi.org/10.3390/molecules25225397