Pestalotiones A–D: Four New Secondary Metabolites from the Plant Endophytic Fungus Pestalotiopsis Theae
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structure Elucidation
2.2. Bioactivities
3. Experimental Section
3.1. General Experimental Procedures
3.2. Fungal Material
3.3. Extraction and Isolation
3.4. Spectroscopic Data (uv and IR, ms)
3.5. MTS Assay
3.6. Antioxidant Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Not available. |
Pos. | 1 | 2 | ||||
---|---|---|---|---|---|---|
δHa (J in Hz) | δCb | HMBCc | δHa (J in Hz) | δCb | HMBCc | |
1 | 134.7, qC | 141.2, qC | ||||
2 | 7.13, d (2.2) | 112.9, CH | 3, 4, 9a, 12 | 118.5, qC | ||
3 | 165.3, qC | 166.7, qC | ||||
4 | 7.30, d (2.2) | 101.9, CH | 2, 3, 4a, 9, 9a | 7.22, s | 101.3, CH | 2, 3, 4a, 9, 9a |
4a | 158.1, qC | 159.5, qC | ||||
5 | 7.45, s | 107.5, CH | 6, 7, 9, 10a, 11 | 6.83, s | 108.2, CH | 7, 8a, 9, 10a, 11 |
6 | 139.4, qC | 150.3, qC | ||||
7 | 7.23, s | 110.6, CH | 5, 6, 8, 8a, 9, 11 | 6.63, s | 112.4, CH | 5, 8, 8a, 11, |
8 | 160.7, qC | 162.3, qC | ||||
8a | 110.1, qC | 107.1, qC | ||||
9 | 179.5, qC | 180.3, qC | ||||
9a | 110.3, qC | 111.7, qC | ||||
10a | 155.3, qC | 156.8, qC | ||||
11 | 165.8, qC | 2.43, s | 22.4, CH3 | 5, 6, 7 | ||
12 | 167.9, qC | 168.4, qC | ||||
13a | 3.00, m | 38.0, CH2 | 2, 14 | |||
13b | 2.87, m | 2, 14 | ||||
14a | 3.56, m | 61.5, CH2 | 13 | |||
14b | 3.50, m | 13 | ||||
CH3O-3 | 3.98, s | 56.8, CH3 | 3 | 4.14, s | 57.9, CH3 | 3 |
CH3O-12 | 3.89, s | 52.8, CH3 | 12 | 3.97, s | 53.1, CH3 | 12 |
HO-8 | 12.3, s | 7, 8, 8a | 12.3, s | 7, 8, 8a |
Pos. | δHa (J in Hz) | δCb | HMBCc |
---|---|---|---|
1 | 110.9, qC | ||
2 | 163.2, qC | ||
3 | 6.13, s | 109.0, CH | 1, 2, 5, 7, 8 |
4 | 149.6, qC | ||
5 | 6.13, s | 109.0, CH | 1, 3, 6, 7, 8 |
6 | 163.2, qC | ||
7 | 201.1, qC | ||
8 | 2.20, s | 22.1, CH3 | 3, 4, 5 |
1′ | 130.6, qC | ||
2′ | 7.17, d (2.4) | 109.0, CH | 1′, 3′, 4′, 6′, 7′ |
3′ | 161.6, qC | ||
4′ | 7.03, d (2.4) | 108.2, CH | 2′, 3′, 5′, 6′ |
5′ | 155.7, qC | ||
6′ | 130.4, qC | ||
7′ | 167.7, qC | ||
8′ | 3.70, s | 52.6, CH3 | 7′ |
9′ | 3.86, s | 56.2, CH3 | 3′ |
1′′ | 5.56, d (4.4) | 103.3, CH | 5′, 2′′, 3′′, 4′′ |
2′′ | 4.00, dd (6.4, 4.4) | 73.3, CH | 1′′ |
3′′ | 3.90, dd (6.4, 3.2) | 70.7, CH | 1′′ |
4′′ | 3.96, dd (6.8, 3.2) | 88.1, CH | |
5′′ | 3.55, m | 63.0, CH2 | 3′′, 4′′ |
Pos. | δHa (J in Hz) | δCb | HMBCc |
---|---|---|---|
1 | 102.1, qC | ||
2 | 158.2, qC | ||
3 | 5.94, d (1.2) | 107.1, CH | 1, 2, 4, 5, 7, 10 |
4 | 146.6, qC | ||
5 | 6.49, d (1.2) | 112.5, CH | 1, 3, 6, 7, 10 |
6 | 162.1, qC | ||
7 | 169.7, qC | ||
8 | 4.47, q (7.2) | 62.4, CH2 | 7, 9 |
9 | 1.40, t (7.2) | 14.3, CH3 | 8 |
10 | 2.16, s | 22.2, CH3 | 3, 4, 5 |
1′ | 125.5, qC | ||
2′ | 7.05, d (3.2) | 107.3, CH | 1′, 3′, 4′, 6′, 7′ |
3′ | 157.5, qC | ||
4′ | 6.81, d (3.2) | 107.3, CH | 2′, 3′, 5′, 6′ |
5′ | 150.7, qC | ||
6′ | 135.4, qC | ||
7′ | 165.4, qC | ||
8′ | 3.74, s | 52.5, CH3 | 7′ |
9′ | 3.84, s | 55.9, CH3 | 3′ |
OH-6 | 10.54, s | 1, 5, 6 | |
OH-5′ | 6.84, br s | 4′, 6′ |
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Guo, L.; Lin, J.; Niu, S.; Liu, S.; Liu, L. Pestalotiones A–D: Four New Secondary Metabolites from the Plant Endophytic Fungus Pestalotiopsis Theae. Molecules 2020, 25, 470. https://doi.org/10.3390/molecules25030470
Guo L, Lin J, Niu S, Liu S, Liu L. Pestalotiones A–D: Four New Secondary Metabolites from the Plant Endophytic Fungus Pestalotiopsis Theae. Molecules. 2020; 25(3):470. https://doi.org/10.3390/molecules25030470
Chicago/Turabian StyleGuo, Longfang, Jie Lin, Shubin Niu, Shuchun Liu, and Ling Liu. 2020. "Pestalotiones A–D: Four New Secondary Metabolites from the Plant Endophytic Fungus Pestalotiopsis Theae" Molecules 25, no. 3: 470. https://doi.org/10.3390/molecules25030470