Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity
Antagonistic Effect of CAPE on CPT-11 and SN38
2.2. Caspase 3/7 Activity
2.3. DNA Damage
2.3.1. Genotoxicity of CAPE
2.3.2. Genotoxicity CAPE + CPT-11 and CAPE + SN38
2.4. Intracellular ROS Determined by H2-DCFDA
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture
4.2.1. Peripheral Blood Lymphocytes (PBLs)
4.2.2. Cancer Cell Lines
4.3. Cytotoxicity
Synergistic or Antagonistic Effect
4.4. Measurement of Caspase-3/7 Activity
4.5. DNA Damage–Alkaline Comet Assay
4.5.1. Samples Preparation and Lysis
4.5.2. Electrophoretic Separation and Staining
4.5.3. Analysis of Comet Assay
4.6. Measurement of Reactive Oxygen Species Generation
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compound are not available from the authors. |
IC50 a | |||||
---|---|---|---|---|---|
CAPE | CPT-11 | SN38 | CAPE + CPT-11 | CAPE + SN38 | |
AGS | 8.0 ± 1.8 µM | 8.0 ± 2.4 µM | 0.8 ± 1.6 µM | 8 ± 1.7 µM | 0.9 ± 0.3 µM |
HCT116 | 8.0 ± 2.1 µM | 4.5 ± 1.9 µM | 0.5 ± 0. 9 µM | 5 ± 2.1 µM | 0.5 ± 0.7 µM |
HT29 | 24.0 ± 2.4 µM | 8.5 ± 2.9 µM | 0.3 ± 1.0µM | 8.5 ± 1.1 µM | 0.25 ± 0.8 µM |
PBLs | ni | 200 ± 5.2 µM | 16 ± 3.2 µM | ni | ni |
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Gajek, G.; Marciniak, B.; Lewkowski, J.; Kontek, R. Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro. Molecules 2020, 25, 658. https://doi.org/10.3390/molecules25030658
Gajek G, Marciniak B, Lewkowski J, Kontek R. Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro. Molecules. 2020; 25(3):658. https://doi.org/10.3390/molecules25030658
Chicago/Turabian StyleGajek, Gabriela, Beata Marciniak, Jarosław Lewkowski, and Renata Kontek. 2020. "Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro" Molecules 25, no. 3: 658. https://doi.org/10.3390/molecules25030658