Anti-Neuraminidase Bioactives from Manggis Hutan (Garcinia celebica L.) Leaves: Partial Purification and Molecular Characterization
Abstract
:1. Introduction
2. Results
2.1. Extraction, Isolation and Bioassay
2.2. Binding Interaction of the Isolated Compound from Garcinia celebica
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Isolation of Compounds from n-Hexane Extract of GCL
4.3. Isolation of Compounds from EtOAc Extract of GCL
4.4. General Experiments and Spectroscopy Methods
4.5. Neuraminidase (NA) Activity
4.6. Molecular Docking Simulation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the G. celebica leaves extracts and catechin (isolated compound) are available from the authors. |
Extract | Partitions | Fractions | Compounds | IC50 * | |
---|---|---|---|---|---|
NA-C. perfringens a | NA-H1N1 b | ||||
MeOH extract | 4.48 µg/mL | 3.65 µg/mL | |||
(17.98% mass) | Hexane extract | 11.04 µg/mL | 74.01 µg/mL | ||
(14.96% w/w) | F1 (5.07%) | nd | nd | ||
F2 (4.5%) | 39.42 µg/mL | nd | |||
F3 (21.40%) | 73.84 µg/mL | 3.243 µg/mL | |||
GC1 (2.20%) | nd | nd | |||
F4 (21.28%) | 8.24 µg/mL | 289.4 µg/mL | |||
GC2 (0.55%) | 81.74 µg/mL | 962.80 µg/mL | |||
GC3 (0.24%) | 142.49 µg/mL | nd | |||
F5 (21.45%) | 277.0 µg/mL | nd | |||
EtOAc extract | 38.39 µg/mL | 48.36 µg/mL | |||
(25.86% w/w) | F1 (4.80%) | nd | nd | ||
F2 (26.39%) | 120.90 µg/mL | nd | |||
F3 (14.21%) | 221.50 µg/mL | 146.49 µg/m | |||
F4 (30.4%) | 16.18 µg/mL | 9.60 µg/mL | |||
GC4 (0.49%) | 17.48 µg/mL | 26.29 µg/mL |
Compound Code | Molecular Structure | Molecular Formula | MW | HBD | HBA | Log P |
---|---|---|---|---|---|---|
GC1 | C30H50O | 426 | 1 | 3 | 7.03 | |
GC2 | C31H48O4 | 502 | 3 | 5 | 5.18 | |
GC3 | C31H48O4 | 485 | 2 | 4 | 6.13 | |
GC4 | C15H14O6 | 290 | 5 | 6 | 1.5 |
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Muchtaridi, M.; Sugijanto, M.; Mohd Gazzali, A.; Wahab, H.A. Anti-Neuraminidase Bioactives from Manggis Hutan (Garcinia celebica L.) Leaves: Partial Purification and Molecular Characterization. Molecules 2020, 25, 821. https://doi.org/10.3390/molecules25040821
Muchtaridi M, Sugijanto M, Mohd Gazzali A, Wahab HA. Anti-Neuraminidase Bioactives from Manggis Hutan (Garcinia celebica L.) Leaves: Partial Purification and Molecular Characterization. Molecules. 2020; 25(4):821. https://doi.org/10.3390/molecules25040821
Chicago/Turabian StyleMuchtaridi, Muchtaridi, Milyadi Sugijanto, Amirah Mohd Gazzali, and Habibah A. Wahab. 2020. "Anti-Neuraminidase Bioactives from Manggis Hutan (Garcinia celebica L.) Leaves: Partial Purification and Molecular Characterization" Molecules 25, no. 4: 821. https://doi.org/10.3390/molecules25040821