Chitosan Oligosaccharides Attenuate Amyloid Formation of hIAPP and Protect Pancreatic β-Cells from Cytotoxicity
Abstract
:1. Introduction
2. Results
2.1. Chitosan Oligosaccharides Preparation
2.2. Fluorescent Assay of hIAPP Aggregation Influenced by COS
2.3. Secondary Structure Analysis of hIAPP Influenced by COS
2.4. Morphologies of hIAPP Aggregates Visualized by Transmission Electron Microscope
2.5. Mechanism Study of hIAPP Aggregation Influenced by COS
2.6. Effect of COS on hIAPP Cytotoxicity
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Purification of Recombinant Enzymes
4.3. Preparation of Chitosan Oligosaccharides
4.4. hIAPP Preparation and Aggregation
4.5. Thioflavine T (ThT) Fluorescence Assay
4.6. Circular Dichroism Spectroscopy
4.7. Transmission Electron Microscopy (TEM)
4.8. Cell Culture
4.9. Cytotoxicity Assay and Cell Cycle Analysis
4.10. Surface Plasmon Resonance (SPR)
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds, COS are available from the authors. |
Sample | t1/2 (h)1 | Lag Time (h)2 | k (h−1)3 | Maximum Intensity (a.u.) 4 |
---|---|---|---|---|
hIAPP | 14.83 ± 0.96 | 4.30 ± 0.36 | 0.19 ± 0.01 | 16060 ± 206.40 |
hIAPP + COS-2.5 mg/mL | 17.63 ± 0.23 | 9.00 ± 0.43 | 0.23 ± 0.012 | 8640 ± 226.50 |
hIAPP + COS-5.0 mg/mL | 15.91 ± 0.76 | 8.47 ± 0.80 | 0.28 ± 0.03 | 6410 ± 124.90 |
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Meng, Q.-Y.; Wang, H.; Cui, Z.-B.; Yu, W.-G.; Lu, X.-Z. Chitosan Oligosaccharides Attenuate Amyloid Formation of hIAPP and Protect Pancreatic β-Cells from Cytotoxicity. Molecules 2020, 25, 1314. https://doi.org/10.3390/molecules25061314
Meng Q-Y, Wang H, Cui Z-B, Yu W-G, Lu X-Z. Chitosan Oligosaccharides Attenuate Amyloid Formation of hIAPP and Protect Pancreatic β-Cells from Cytotoxicity. Molecules. 2020; 25(6):1314. https://doi.org/10.3390/molecules25061314
Chicago/Turabian StyleMeng, Qin-Yu, Hua Wang, Zi-Bo Cui, Wen-Gong Yu, and Xin-Zhi Lu. 2020. "Chitosan Oligosaccharides Attenuate Amyloid Formation of hIAPP and Protect Pancreatic β-Cells from Cytotoxicity" Molecules 25, no. 6: 1314. https://doi.org/10.3390/molecules25061314