Multi-Targeting Bioactive Compounds Extracted from Essential Oils as Kinase Inhibitors

Round 1

Reviewer 1 Report

The manuscript can be accepted for publication in its current form.

In the manuscript “ Multi-Targeting bioactive compounds extracted from Essential Oils as kinase inhibitors” the authors applied computational methods in order to discover new potential anticancer scaffolds obtained from natural essential oils that could be considered a new and sustainable resource of kinase inhibitors. The cinnamyl cinnamate was tested on different cell lines with the aim to verify the antiproliferative activity. The manuscript is interesting; the paper is well written, and the conclusions consistent with the evidence and arguments presented.

Author Response

We thank the reviewer for the appreciation, and we are glad that our work has been judged interesting and well written.

Reviewer 2 Report

This is an interesting paper reporting structure and structure-based screening of some bioactive compounds from Essential Oils as potential kinase inhibitors. Given the importance of this activity for ant-cancer drugs, this work is remarkably interesting for anti-cancer drugs development. To test selected compounds as potential anticancer agents, it was performed the investigation of viability of different cancer cell lines using one of the compounds, cinnamyl cinnamate.

Minor point.

In the Abstract the test is claimed as verification of antiproliferative activity. In the text it is presented as viability test. In the latter case, the Authors use very unspecific way to determine cell viability, and they don’t determine type of cell death.

Author Response

We thank the reviewer for his/her suggestion. According to such indications, we have modified “viability” in antiproliferative in line 239, 244,  in the caption and the wording on the graphs in the Figure 4.