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Article

Reversible Dimerization of Human Serum Albumin

by
Alexey Chubarov
1,2,*,
Anna Spitsyna
2,3,
Olesya Krumkacheva
2,4,*,
Dmitry Mitin
1,2,
Daniil Suvorov
1,2,
Victor Tormyshev
4,
Matvey Fedin
2,4,
Michael K. Bowman
4,5,* and
Elena Bagryanskaya
4,*
1
Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia
2
Novosibirsk State University, 630090 Novosibirsk, Russia
3
N.N. Vorozhtsov Institute of Organic Chemistry SB RAS, 630090 Novosibirsk, Russia
4
International Tomography Center SB RAS, 630090 Novosibirsk, Russia
5
Department of Chemistry & Biochemistry, University of Alabama, Tuscaloosa, AL 35487, USA
*
Authors to whom correspondence should be addressed.
Molecules 2021, 26(1), 108; https://doi.org/10.3390/molecules26010108
Submission received: 11 December 2020 / Revised: 24 December 2020 / Accepted: 25 December 2020 / Published: 29 December 2020
(This article belongs to the Special Issue Stable Radicals: Synthesis, Structure and Applications: A Theme Issue in Honor of Prof. Dr. Leonid Volodarsky)
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Abstract

Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to detect and characterize reversible non-covalent dimers of Human Serum Albumin (HSA), the most abundant protein in human plasma. The spin labels, MTSL and OX063, were attached to Cys-34 and these chemical modifications of Cys-34 did affect the dimerization of HSA, indicating that other post-translational modifications can modulate dimer formation. At physiologically relevant concentrations, HSA does form weak, non-covalent dimers with a well-defined structure. Dimer formation is readily reversible into monomers. Dimerization is very relevant to the role of HSA in the transport, binding, and other physiological processes.
Keywords: human serum albumin; pulse dipole EPR; aggregation human serum albumin; pulse dipole EPR; aggregation

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MDPI and ACS Style

Chubarov, A.; Spitsyna, A.; Krumkacheva, O.; Mitin, D.; Suvorov, D.; Tormyshev, V.; Fedin, M.; Bowman, M.K.; Bagryanskaya, E. Reversible Dimerization of Human Serum Albumin. Molecules 2021, 26, 108. https://doi.org/10.3390/molecules26010108

AMA Style

Chubarov A, Spitsyna A, Krumkacheva O, Mitin D, Suvorov D, Tormyshev V, Fedin M, Bowman MK, Bagryanskaya E. Reversible Dimerization of Human Serum Albumin. Molecules. 2021; 26(1):108. https://doi.org/10.3390/molecules26010108

Chicago/Turabian Style

Chubarov, Alexey, Anna Spitsyna, Olesya Krumkacheva, Dmitry Mitin, Daniil Suvorov, Victor Tormyshev, Matvey Fedin, Michael K. Bowman, and Elena Bagryanskaya. 2021. "Reversible Dimerization of Human Serum Albumin" Molecules 26, no. 1: 108. https://doi.org/10.3390/molecules26010108

APA Style

Chubarov, A., Spitsyna, A., Krumkacheva, O., Mitin, D., Suvorov, D., Tormyshev, V., Fedin, M., Bowman, M. K., & Bagryanskaya, E. (2021). Reversible Dimerization of Human Serum Albumin. Molecules, 26(1), 108. https://doi.org/10.3390/molecules26010108

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