Blockade of NF-κB Translocation and of RANKL/RANK Interaction Decreases the Frequency of Th2 and Th17 Cells Capable of IL-4 and IL-17 Production, Respectively, in a Mouse Model of Allergic Asthma
Abstract
:1. Introduction
2. Results
2.1. DHMEQ and OPG Prevent OVA-Induced Increase in the Relative and Absolute Counts of IL-4-, IL-10- and IL-17-Producing Non-Treg CD4+ T Cells in the Lungs and Administration of BMS Prevents the Absolute, but Not Relative, Increase in the Number of These Cells
2.2. BMS, DHMEQ and OPG Prevent OVA-Induced Increase in the Absolute Counts of IL-10- and TGF-β-Producing Treg Cells in the Lungs
2.3. BMS, DHMEQ and OPG Prevent OVA-Induced Increase in the Absolute Counts of IL-4- and IL-17-Producing CD8+ T Cells in the Lungs
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Antigen Immunization, Airway Challenge and Treatment Protocol
4.3. Isolation of Inductive and Effector Site Lymphocytes and Culture Conditions
4.3.1. MLNs
4.3.2. Lungs
4.3.3. Ex Vivo Stimulation of Cytokine Production
4.4. Flow Cytometry
4.4.1. Extracellular Staining
4.4.2. Intracellular Staining
4.5. FACS Acquisition and Data Analysis
4.6. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Gregorczyk, I.; Jasiecka-Mikołajczyk, A.; Maślanka, T. Blockade of NF-κB Translocation and of RANKL/RANK Interaction Decreases the Frequency of Th2 and Th17 Cells Capable of IL-4 and IL-17 Production, Respectively, in a Mouse Model of Allergic Asthma. Molecules 2021, 26, 3117. https://doi.org/10.3390/molecules26113117
Gregorczyk I, Jasiecka-Mikołajczyk A, Maślanka T. Blockade of NF-κB Translocation and of RANKL/RANK Interaction Decreases the Frequency of Th2 and Th17 Cells Capable of IL-4 and IL-17 Production, Respectively, in a Mouse Model of Allergic Asthma. Molecules. 2021; 26(11):3117. https://doi.org/10.3390/molecules26113117
Chicago/Turabian StyleGregorczyk, Izabela, Agnieszka Jasiecka-Mikołajczyk, and Tomasz Maślanka. 2021. "Blockade of NF-κB Translocation and of RANKL/RANK Interaction Decreases the Frequency of Th2 and Th17 Cells Capable of IL-4 and IL-17 Production, Respectively, in a Mouse Model of Allergic Asthma" Molecules 26, no. 11: 3117. https://doi.org/10.3390/molecules26113117