Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
- DNA probe = (5′-HS(CH2)6GTCATTATGTGCTGCCATATCTACTT-CAGA-3′);
- DNA complementary target = (5′-TCTGAAGTAGATATGGCAGCACATAATGAC-3′);
- DNA single-based mismatch target = (5′-TCTGAAATAGATATGGCAGCACATAATGAC-3′).
2.2. DNA Probe Immobilization and Hybridization with DNA Target
2.3. Electrochemical Measurements
2.4. The Current Relaxation Method
2.5. Sensing Circuit
3. Results and Discussion
3.1. Step Potential
3.2. Analytical Performance
3.3. Specificity of the DNA Biosensor
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Irelax/µA | Rtotal/kΩ | |
---|---|---|
Bare Au electrode | 22.2 | 1.3 |
ssDNA | 11.9 | 2.4 |
dsDNA | 4.3 | 6.6 |
HPV Type | Technique | Sensor Platform | Detection Limit | Response Time | Ref. |
---|---|---|---|---|---|
HPV-16 | DPV | PGE | 1.49 nM | 40 s | [43] |
HPV-16 | SWV | Carbon surface/chitosan | 4 nM | 10 s | [44] |
HPV-16 | EIS, SWV | Paper base/G-PANI | 2.3 nM | 17 min, 15 s | [45] |
HPV-16 | CV | GCE/CNO | 0.50 nM | 7 min | [46] |
HPV-45 | CA | Gold surface | 110 pM | 60 s | [47] |
HPV-16 | EIS | GCE/gold nanosheet | 0.15 pM | 17 min | [48] |
HPV-18 | SWV | GCE/carboxyphenyl layer | 1.2 × 10−5 nM | 10 s | [49] |
HPV-16 | CA | Gold surface | 2.39 nM | 750 µs | This work |
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Espinosa, J.R.; Galván, M.; Quiñones, A.S.; Ayala, J.L.; Ávila, V.; Durón, S.M. Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16. Molecules 2021, 26, 3436. https://doi.org/10.3390/molecules26113436
Espinosa JR, Galván M, Quiñones AS, Ayala JL, Ávila V, Durón SM. Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16. Molecules. 2021; 26(11):3436. https://doi.org/10.3390/molecules26113436
Chicago/Turabian StyleEspinosa, José R., Marisol Galván, Arturo S. Quiñones, Jorge L. Ayala, Verónica Ávila, and Sergio M. Durón. 2021. "Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16" Molecules 26, no. 11: 3436. https://doi.org/10.3390/molecules26113436