Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss
Abstract
:1. Introduction
2. Results
2.1. USMBs Enhanced the Delivery Efficiency of IGF-1 to the Inner Ear
2.2. The Combination of USMBs and IGF-1 Had a Better Therapeutic Effect on NIHL
2.3. USMBs Enhanced the Therapeutic Effect of IGF-1 by Promoting Activation of IGF-1 Signaling Pathways in the Cochlea
3. Discussion
4. Materials and Methods
4.1. Animals and Study Design
4.2. Preparation of MBs and Ultrasound Exposure
4.3. Surgical Technique
4.4. Noise Exposure
4.5. ELISA Analysis of rhIGF-1 Levels
4.6. Quantitative PCR Analysis of Mapk1, Mapk3 and Akt1 Gene Expression
4.7. Surface Preparation of the Organ of Corti
4.8. Analysis of Outer Hair Cell Survival and Synaptic Ribbon Counts
4.9. Cochlear Cryosections and Immunostaining for Phospho-ERK1/2 and Phospho-AKT
4.10. Auditory Brainstem Response Recording
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Stimulus | Threshold Shift (dB) 14 Days after Noise Exposure | Threshold Shift (dB) 28 Days after Noise Exposure | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Saline | RWS | USM | Saline | RWS | USM | |||||||
Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
8 kHz | 56.7 | 2.11 | 50 | 4.28 | 43.3 | 4.22 | 53.3 | 2.11 | 43.3 | 2.47 | 32.5 | 2.81 |
16 kHz | 44.2 | 3.00 | 36.7 | 3.33 | 27.5 | 2.50 | 43.3 | 3.07 | 33.3 | 2.11 | 24.2 | 1.54 |
24 kHz | 38.3 | 2.11 | 26.7 | 1.67 | 24.2 | 3.00 | 35.8 | 1.54 | 26.7 | 2.11 | 23.3 | 2.11 |
32 kHz | 35.8 | 3.27 | 31.7 | 3.58 | 29.2 | 5.07 | 35.8 | 2.39 | 25.8 | 2.39 | 24.2 | 4.17 |
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Lin, Y.-C.; Lin, Y.-Y.; Chen, H.-C.; Kuo, C.-Y.; Liao, A.-H.; Chou, Y.-L.; Hung, C.-L.; Shih, C.-P.; Wang, C.-H. Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss. Molecules 2021, 26, 3626. https://doi.org/10.3390/molecules26123626
Lin Y-C, Lin Y-Y, Chen H-C, Kuo C-Y, Liao A-H, Chou Y-L, Hung C-L, Shih C-P, Wang C-H. Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss. Molecules. 2021; 26(12):3626. https://doi.org/10.3390/molecules26123626
Chicago/Turabian StyleLin, Yi-Chun, Yuan-Yung Lin, Hsin-Chien Chen, Chao-Yin Kuo, Ai-Ho Liao, Ying-Liang Chou, Chia-Lien Hung, Cheng-Ping Shih, and Chih-Hung Wang. 2021. "Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss" Molecules 26, no. 12: 3626. https://doi.org/10.3390/molecules26123626