Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation of FX by ODS Column Chromatography
2.2. Effects of Vacuum Evaporation on FX-Rich Fraction
2.3. Efficiency of Ethanol Precipitation in the Purification of FX
2.4. Identification of FX
3. Materials and Methods
3.1. Materials
3.2. Apparatus
3.3. FX-Containing Crude Preparation
3.4. ODS Column Chromatographic Experiments
3.5. Vacuum Evaporation of FX-Rich Fraction
3.6. Purification of FX by Precipitation
3.7. Characterization
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Kim, S.K.; Pangestuti, R. Biological activities and potential health benefits of fucoxanthin derived from marine brown algae. Adv. Food Nutr. Res. 2011, 64, 111–128. [Google Scholar]
- Petrushkina, M.; Gusev, E.; Sorokin, B.; Zotko, N.; Mamaeva, A.; Filimonova, A.; Kulikovskiy, M.; Maltsev, Y.; Yampolsky, I.; Guglya, E.; et al. Fucoxanthin production by heterokont microalgae. Algal Res. 2017, 24, 387–393. [Google Scholar] [CrossRef]
- Rengarajan, T.; Rajendran, P.; Nandakumar, N.; Balasubramanian, M.P.; Nishigaki, I. Cancer preventive efficacy of marine carotenoid fucoxanthin: Cell cycle arrest and apoptosis. Nutrients 2013, 5, 4978–4989. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Peng, J.; Yuan, J.P.; Wu, C.F.; Wang, J.H. Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: Metabolism and bioactivities relevant to human health. Mar. Drugs 2011, 9, 1806–1828. [Google Scholar] [CrossRef] [PubMed]
- Maoka, T.; Fujiwara, Y.; Hashimoto, K.; Akimoto, N. Characterization of fucoxanthin and fucoxanthinol esters in the Chinese surf clam, Mactra chinensis. J. Agric. Food Chem. 2007, 55, 1563–1567. [Google Scholar] [CrossRef]
- Mikami, K.; Hosokawa, M. Biosynthetic pathway and health benefits of fucoxanthin, an algae-specific xanthophyll in brown seaweeds. Int. J. Mol. Sci. 2013, 14, 13763–13781. [Google Scholar] [CrossRef] [Green Version]
- Zhao, D.; Kim, S.M.; Pan, C.H.; Chung, D. Effects of heating, aerial exposure and illumination on stability of fucoxanthin in canola oil. Food Chem. 2014, 145, 505–513. [Google Scholar] [CrossRef]
- Sun, X.; Xu, Y.; Zhao, L.; Yan, H.; Wang, S.; Wang, D. The stability and bioaccessibility of fucoxanthin in spray-dried microcapsules based on various biopolymers. RSC Adv. 2018, 8, 35139–35149. [Google Scholar] [CrossRef] [Green Version]
- Bae, M.; Kim, M.B.; Park, Y.K.; Lee, J.Y. Health benefits of fucoxanthin in the prevention of chronic diseases. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 2020, 1865, 158618. [Google Scholar] [CrossRef]
- D’Orazio, N.; Gemello, E.; Gammone, M.A.; de Girolamo, M.; Ficoneri, C.; Riccioni, G. Fucoxantin: A treasure from the sea. Mar. Drugs 2012, 10, 604–616. [Google Scholar] [CrossRef] [Green Version]
- Shannon, E.; Abu-Ghannam, N. Optimisation of fucoxanthin extraction from irish seaweeds by response surface methodology. J. Appl. Phycol. 2016, 29, 1027–1036. [Google Scholar] [CrossRef] [Green Version]
- Lourenco-Lopes, C.; Garcia-Oliveira, P.; Carpena, M.; Fraga-Corral, M.; Jimenez-Lopez, C.; Pereira, A.G.; Prieto, M.A.; Simal-Gandara, J. Scientific approaches on extraction, purification and stability for the commercialization of fucoxanthin recovered from brown algae. Foods 2020, 9, 1113. [Google Scholar] [CrossRef]
- Piovan, A.; Seraglia, R.; Bresin, B.; Caniato, R.; Filippini, R. Fucoxanthin from Undaria pinnatifida: Photostability and coextractive effects. Molecules 2013, 18, 6298–6310. [Google Scholar] [CrossRef] [Green Version]
- Gómez-Loredo, A.; Benavides, J.; Rito-Palomares, M. Partition behavior of fucoxanthin in ethanol-potassium phosphate two-phase systems. J. Chem. Technol. Biotechnol. 2014, 89, 1637–1645. [Google Scholar] [CrossRef]
- Raji, V.; Loganathan, C.; Sadhasivam, G.; Kandasamy, S.; Poomani, K.; Thayumanavan, P. Purification of fucoxanthin from Sargassum wightii Greville and understanding the inhibition of angiotensin 1-converting enzyme: An in vitro and in silico studies. Int. J. Biol. Macromol. 2020, 148, 696–703. [Google Scholar] [CrossRef] [PubMed]
- Mohamadnia, S.; Tavakoli, O.; Faramarzi, M.A.; Shamsollahi, Z. Production of fucoxanthin by the microalga Tisochrysis lutea: A review of recent developments. Aquaculture 2020, 516, 734637. [Google Scholar] [CrossRef]
- Xiao, X.; Si, X.; Yuan, Z.; Xu, X.; Li, G. Isolation of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography. J. Sep. Sci. 2012, 35, 2313–2317. [Google Scholar] [CrossRef] [PubMed]
- Sun, P.; Wong, C.C.; Li, Y.; He, Y.; Mao, X.; Wu, T.; Ren, Y.; Chen, F. A novel strategy for isolation and purification of fucoxanthinol and fucoxanthin from the Diatom nitzschia laevis. Food Chem. 2019, 277, 566–572. [Google Scholar] [CrossRef]
- Xia, M.; Liu, C.; Gao, L.; Lu, Y. One-step preparative separation of phytosterols from edible brown seaweed Sargassum horneri by high-speed countercurrent chromatography. Mar. Drugs 2019, 17, 691. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Komatsu, T.; Fukuda, M.; Mikami, A.; Mizuno, S.; Kantachumpoo, A.; Tanoue, H.; Kawamiya, M. Possible change in distribution of seaweed, Sargassum horneri, in northeast Asia under A2 scenario of global warming and consequent effect on some fish. Mar. Pollut. Bull. 2014, 85, 317–324. [Google Scholar] [CrossRef]
- Airanthi, M.K.; Sasaki, N.; Iwasaki, S.; Baba, N.; Abe, M.; Hosokawa, M.; Miyashita, K. Effect of brown seaweed lipids on fatty acid composition and lipid hydroperoxide levels of mouse liver. J. Agric. Food Chem. 2011, 59, 4156–4163. [Google Scholar] [CrossRef]
- Saraswati; Giriwono, P.E.; Iskandriati, D.; Tan, C.P.; Andarwulan, N. Sargassum seaweed as a source of anti-inflammatory substances and the potential insight of the tropical species: A review. Mar. Drugs 2019, 17, 590. [Google Scholar] [CrossRef] [Green Version]
- Han, E.J.; Kim, H.S.; Sanjeewa, K.K.A.; Jung, K.; Jee, Y.; Jeon, Y.J.; Fernando, I.P.S.; Ahn, G. Sargassum horneri as a functional food ameliorated IgE/BSA-Induced mast cell activation and passive cutaneous anaphylaxis in mice. Mar. Drugs 2020, 18, 594. [Google Scholar] [CrossRef] [PubMed]
- Lee, J.H.; Kim, H.J.; Jee, Y.; Jeon, Y.J.; Kim, H.J. Antioxidant potential of Sargassum horneri extract against urban particulate matter-induced oxidation. Food Sci. Biotechnol. 2020, 29, 855–865. [Google Scholar] [CrossRef] [PubMed]
- Silchenko, A.S.; Rasin, A.B.; Kusaykin, M.I.; Kalinovsky, A.I.; Miansong, Z.; Changheng, L.; Malyarenko, O.; Zueva, A.O.; Zvyagintseva, T.N.; Ermakova, S.P. Structure, enzymatic transformation, anticancer activity of fucoidan and sulphated fucooligosaccharides from Sargassum horneri. Carbohydr. Polym. 2017, 175, 654–660. [Google Scholar] [CrossRef] [PubMed]
- Zarekarizi, A.; Hoffmann, L.; Burritt, D. Approaches for the sustainable production of fucoxanthin, a xanthophyll with potential health benefits. J. Appl. Phycol. 2018, 31, 281–299. [Google Scholar] [CrossRef]
- Nomura, M.; Kamogawa, H.; Susanto, E.; Kawagoe, C.; Yasui, H.; Saga, N.; Hosokawa, M.; Miyashita, K. Seasonal variations of total lipids, fatty acid composition, and fucoxanthin contents of Sargassum horneri (Turner) and Cystoseira hakodatensis (Yendo) from the northern seashore of Japan. J. Appl. Phycol. 2012, 25, 1159–1169. [Google Scholar] [CrossRef]
- Yamamura, R.; Shimomura, Y. Industrial high-performance liquid chromatography purification of docosahexaenoic acid ethyl ester and docosapentaenoic acid ethyl ester from single-cell oil. J. Am. Oil Chem. Soc. 1997, 74, 1435–1440. [Google Scholar] [CrossRef]
- Cano, M.E.; Garcia-Martin, A.; Ladero, M.; Lesur, D.; Pilard, S.; Kovensky, J. A simple procedure to obtain a medium-size oligogalacturonic acids fraction from orange peel and apple pomace wastes. Food Chem. 2021, 346, 128909. [Google Scholar] [CrossRef]
- Gao, Y.; Guo, Q.; Zhang, K.; Wang, N.; Li, C.; Li, Z.; Zhang, A.; Wang, C. Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics. Int. J. Biol. Macromol. 2020, 165 (Pt B), 1960–1969. [Google Scholar] [CrossRef]
- Tai, Y.; Shen, J.; Luo, Y.; Qu, H.; Gong, X. Research progress on the ethanol precipitation process of traditional Chinese medicine. Chin. Med. 2020, 15, 84. [Google Scholar] [CrossRef] [PubMed]
- Jyothi, M.S.; Kakarla, R.R.; Soontarapa, K.; Naveenc, S.; Anjanapura, V.R.; Raghavendra, V.K.; Suhase, D.P.; Nagaraj, P.S.; Mallikarjuna, N.N.; Tejraj, M.A. Membranes for dehydration of alcohols via pervaporation. J. Environ. Manag. 2019, 242, 415–429. [Google Scholar] [CrossRef]
- Confalonieri, G.; Quartieri, S.; Vezzalini, G.; Tabacchi, G.; Fois, E.; Daou, T.J.; Arletti, R. Differential penetration of ethanol and water in Si-chabazite: High pressure dehydration of azeotrope solution. Microporous Mesoporous Mater. 2019, 284, 161–169. [Google Scholar] [CrossRef]
- Dong, X.; Cao, Y.; Wang, N.; Wang, P.; Li., M. Systematic study on solubility of chrysin in different organic solvents: The synergistic effect of multiple intermolecular interactions on the dissolution process. J. Mol. Liq. 2021, 325, 115180. [Google Scholar] [CrossRef]
- Mori, K.; Ooi, T.; Hiraoka, M.; Oka, N.; Hamada, H.; Tamura, M.; Kusumi, T. Fucoxanthin and its metabolites in edible brown algae cultivated in deep seawater. Mar. Drugs 2004, 2, 63–72. [Google Scholar] [CrossRef] [Green Version]
- Haugan, J.A.; Liaaen-Jensen, S. Isolation and characterisation of four allenic (6′S)-isomers of fucoxanthin. Tetrahedron Lett. 1994, 35, 2245–2248. [Google Scholar] [CrossRef]
- Mok, I.K.; Yoon, J.R.; Pan, C.H.; Kim, S.M. Development, quantification, method validation, and stability study of a novel fucoxanthin-fortified milk. J. Agric. Food Chem. 2016, 64, 6196–6202. [Google Scholar] [CrossRef] [PubMed]
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Ye, Y.; Sun, J.; Wang, L.; Zhu, J.; Cui, W.; Hou, H.; Zhang, J.; Zhou, C.; Yan, X. Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation. Molecules 2021, 26, 3777. https://doi.org/10.3390/molecules26133777
Ye Y, Sun J, Wang L, Zhu J, Cui W, Hou H, Zhang J, Zhou C, Yan X. Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation. Molecules. 2021; 26(13):3777. https://doi.org/10.3390/molecules26133777
Chicago/Turabian StyleYe, Yuemei, Jingwen Sun, Liting Wang, Junwang Zhu, Wei Cui, Hongyan Hou, Jinrong Zhang, Chengxu Zhou, and Xiaojun Yan. 2021. "Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation" Molecules 26, no. 13: 3777. https://doi.org/10.3390/molecules26133777