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Article

Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

by
Pratama Jujur Wibawa
1,*,
Muhammad Nur
2,
Mukhammad Asy’ari
1,
Wijanarka Wijanarka
3,
Heru Susanto
4,
Heri Sutanto
2 and
Hadi Nur
5,6
1
Department of Chemistry, Faculty of Sciences and Mathematics, Diponegoro University, Jalan Prof. H. Soedarto, SH. No.1 Tembalang, Semarang 50275, Indonesia
2
Department of Physics, Faculty of Sciences and Mathematics, Diponegoro University, Jalan Prof. H. Soedarto, SH. No.1 Tembalang, Semarang 50275, Indonesia
3
Department of Biology, Faculty of Sciences and Mathematics, Diponegoro University, Jalan Prof. H. Soedarto, SH. No.1 Tembalang, Semarang 50275, Indonesia
4
Department of Chemical Engineering, Faculty of Enginering, Diponegoro University, Jalan Prof. H. Soedarto, SH. No.1 Tembalang, Semarang 50275, Indonesia
5
Center for Sustainable Nanomaterials, Inbu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, Skudai 81310, Johor, Malaysia
6
Central Laboratory of Minerals and Advanced Materials, Faculty of Mathematica and Natural Sciences, State University of Malang, Malang 65145, Indonesia
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(13), 3790; https://doi.org/10.3390/molecules26133790
Submission received: 15 May 2021 / Revised: 6 June 2021 / Accepted: 13 June 2021 / Published: 22 June 2021
(This article belongs to the Special Issue Activated Carbons—Production and Applications)
Browse Figures
Review Reports Versions Notes

Abstract

:
This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

Graphical Abstract

1. Introduction

It has been widely known that silver nanoparticles (AgNPs) have functional antibacterial properties towards wide spectrum ones from Gram-positive up to Gram-negative bacteria with low risk for human health [1,2,3]. Many researchers have used AgNPs for a long time as antibacterial agents in various industrials products such as cosmetics [4,5], food storage, packaging [6,7], health devices industry [8,9], textile coatings [10,11], etc. On the other hand, it has been also widely known that both pathogenic bacteria of Bacillus subtilis (B. subtilis), and Escherichia coli (E. coli) as the representatives of Gram-positive and Gram-negative bacteria, respectively, are commonly present ubiquitously in various natural environment bodies such as soil, wastewater, and air [12,13]. Due to these media frequently being in contact with humans, the bacteria can reach the human body and subsequently generate many infectious and various diseases. To avoid this dangerous threat, one can use an enhanced high effective antibacterial agent of silver nanoparticles (AgNPs) via preemptive strategy using activated carbon nanoparticles (ACNPs) coated on human clothing materials such as cotton and polyester fabrics.
Unfortunately, almost all the proposed antibacterial mechanisms of AgNPs releasing reactive oxygen species (ROS) radicals [14,15,16] can damage the human cell and induce serious degenerative diseases [17]. To minimize these risks, it is compulsory to use a suitable radical scavenger in using AgNPs as the antibacterial agent in many various applications. In conjunction with that, metal oxide particles such as Aluminum oxide (Al2O3), Cerium oxide (CeO2), and Yttrium oxide (Y2O3) could be applied for the ROS catching [14]. However, metal oxides are commonly expensive in price, not environmentally friendly, and not easy to produce. Therefore, utilization of other high potential ROS adsorbers that are cheaper in price, renewable in sources, sustainable in their availability, safer for humans, and environmental benign in properties, is a must. In this context, activated carbon nanoparticles (ACNPs) have become the best alternative material, fulfilling all the requirements for the ROS adsorber.
As is widely known, ACNPs can be produced quickly from any biomass of plantation, agriculture, and forestry wastes [18,19,20,21], even from industrial livestock wastes [22]. This situation provides remarkable contribution in reducing severe environmental damage. In addition, the high porosity of the ACNPs with various basins in nano-scale size and depths formed on its surface [18] make the ACNPs the most promising material to skip out the ROS all at once to enhance the antibacterial activity of the AgNPs through the synergistic effect mechanism. The possibility of the ROS adsorption process would happen on the surface of the ACNPs, supported by the facts that activated carbon-based materials have been used to adsorb carbon dioxide (CO2) [23], hydrogen peroxide (H2O2) [24], hydrogen (H2) [25], and environmental benign organic molecules [26]. The relationship between surface morphology and potential adsorption of the activated carbon-based materials has been reviewed in detail [27,28,29,30]. This paper comprehensively describes the results of the investigation of the role of ACNPs in enhancing the antibacterial activity of AgNPs towards E. coli and S. aureus bacteria, especially when it was superimposed onto the cotton and polyester fabrics.

2. Experimental Section

2.1. Materials

This research used local commercially available carbon black fine powder; Silver nitrate (AgNO3) p.a; Distilled water; Phosphoric acid (H3PO4) p.a; Sodium hydroxide (NaOH) p.a. Agarose powder; Yeast extract; Peptone; Sodium chloride (NaCl) p.a; Amoxicillin, p.a.; Whatman filter paper No.41. Sigma-Aldrich, Germany, produced all the chemicals.

2.2. Equipment

Commonly used laboratory glassware; Autoclave sterilizer (Laboratory specification, Linden, Germany); Oven (Cosmos CO-9919, North Jakarta, Indonesia), Furnace (Vulcan 3-130, USA), Ultrasonic cleaner (Krisbow CD4862, Shenzhen, China), pH meter (Senz pH Scientific Trans Instrument, Singapore), Analytical Scanning Electron Microscope-Energy Dispersive X-ray (SEM-EDX, JEOL JSM-6510LA, Tokyo, Japan), Fourier-Transform Infrared Spectrometer (FTIR Perkin Elmer, Waltham, MA, USA). UV-Vis spectrometer (UV-1280, Shimadzu, Japan). Transmission Electron Microscope (TEM, Jeol 1400, Tokyo, Japan); Digital camera microscope (DGM, Motic Moticam T, Hong Kong, China).

2.3. Fabrication of Activated Carbon Black Nanoparticles (ACNPs)

We used the ACBMPs that had already been fabricated, according to reference [18]. However, only ACNPs produced from the activation process of using H3PO4 with a 1:1 mass ratio followed by thermal activation at 400 °C for 20 min were used. We then used the ACNPs sample after pH neutral was reached due to a series of neutralization processes employing a 0.5 M NaOH solution and washed with distilled water, which were adequately performed. Finally, we determined the particle sizes of ACNPs with a minor bit of adjustment by Tunnelling Electron Microscope (TEM), which worked according to reference [31].

2.4. Synthesis of Silver Nanoparticles (AgNPs) Using Aqueous-Aloe Vera Extract

Firstly, we washed about 25 g fresh aloe vera leaves of 1 cm × 1 cm in size using distilled water and chopped it in a blender machine (National, Made in Indonesia). Subsequently, we heated it with 100 mL distilled water of 90 °C, stirred at 3000 rpm for 2 h at atmospheric pressure. This mixture was incubated overnight (24 h) at room temperature and filtered using a Whatman 41 filter paper or its equivalent, held in a dark bottle until it was used and reported as a 25% w/w extract of aqueous aloe vera. After that, we diluted the extract to 5% w/w distilled water of 100 mL volume. According to reference [32] protocol, the presence of flavonoid, saponin, and tannin compounds, which are the key secondary metabolites of the aloe vera extract, were justified.
On the other hand, about 1.7 g AgNO3 was dissolved correctly into 50 mL distilled water in a 100 mL glass beaker. It was then gently poured in a 100 mL volumetric flask and subsequently added with distilled water up to the given mark, and it was vigorously shaken for a few seconds and kept in the dark bottle until used, with measurements of 0.1 M of 100 mL AgNO3. Furthermore, 10 mL aloe vera extract of 5% in concentration was mixed correctly with 10 mL AgNO3 of 0.1 M in concentration. We heated this mixture at 100 °C for 15 min, and let it cool at room temperature, before being agitated with ultrasonic wave (40 kHz, 2 × 50 watt) for 10 min. Finally, it was stored in a dark bottle for five days at room temperature. The developed AgNPs were then separated for 10 min by centrifugation at 5000 rpm and then dispersed again in 10 mL of distilled water and stored in a refrigerator until being used.
We justify the success of the synthesis of AgNPs by using UV-Vis’s spectrometer scanning run at the wavelength range of 250 to 700 nm. The maximum wavelength at about 430 to 500 nm was confirmed when the AgNPs formed. Besides those, the existence of flavonoid, saponin, and tannin representative compounds that stabilized the AgNPs might form core-shell nanostructure was analyzed using FTIR spectrometer equipped by germanium universal attenuated total reflectance (ATR) sample holder system. In this case, the sample of about 100 μL AgNPs was directly introduced on the ATR surface, then subsequently pressed at 3-newton force by the device analytical system and was three-time scanned at 5500 to 435 cm−1 default wavenumber range with resolution grade set up 4.

2.5. Incorporating AgNPs on ACNPs Surface

About 10 mL aqueous colloidal AgNPs produced from Section 2.4 were mixed correctly with 90 mL aqueous colloidal ACNPs produced from Section 2.3. The mixture was stirred at 3000 rpm with a magnetic stirrer for 1 h, followed by ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min. It was then centrifuged at room temperature for around 24 h (overnight), then centrifuged at 3000 rotations per minute (rpm). The collected material had been isolated from its corresponding liquid by the decantation method. The collected material was then dried using an electric furnace at 110 °C until constant in weight and heated again in an electric furnace at 500 °C under vacuum for approximately 2 h. Finally, we added 50 mL of distilled water into the material and stored it in the dark bottle until used.

2.6. Surface Morphology Confirmation and Particles Size Determination of ACNPs; AgNPs and AgNPs-Immobilized ACNPs

Surface morphology and particle size of ACNPs, AgNPs, and AgNPs-immobilized ACNPs materials were confirmed by using TEM operated according to reference [30] with a little bit of modification depending on the samples analyzed. In this relationship, the manufactured aqueous colloidal ACNPs, AgNPs, and AgNPs-immobilized ACNPs were each independently deposited on a TEM sample holder grid for about 100 μL. The excess liquid was extracted after about 5 min by blotting with a filter paper, allowing it to dry in an ambient environment. The samples were observed using TEM operated at 100 kV accelerating voltage.

2.7. Antibacterial Activity Test

About 1.5 g agarose, 0.2 g yeast extract, 0.4 g peptone, and 0.4 g NaCl were correctly mixed with 50 mL distilled water. This mixture was then heated in an Autoclave at 121 °C for 20 min. At this moment, the reaction tubes and Petri dishes, every two units, were also sterilized in the Autoclave. After that, the two reaction tubes were gently poured into about 4 mL of the liquid culture, and they were slightly tilted until the culture solidified. The rest of the culture was gently poured into the two Petri dishes with the same portion and were kept solidified at room temperature. Technically, the bacterial medium culture was prepared in a laminar cupboard under a microorganisms-free atmosphere.
On the other hand, about 0.4 g peptone, 0.2 g yeast extract, and 0.4 g NaCl were mixed correctly with 50 mL distilled water. This mixture was then sterilized in an Electrical Autoclave Sterilizer at 121 °C for 30 min. Besides those, about 1 μL of culture stock of S. aureus bacteria and that of E. coli were independently re-cultured on the already prepared solid medium enriched with nutrient agar by using an ose needle incubated at 37 °C for 24 h in a slightly tilted position. After that, both regenerated S. aureus and E. coli bacteria cultures were further cultured in the already prepared liquid medium enriched with nutrient ager using an ose needle. These cultures were then incubated at 37 °C for 24 h in a microorganism-free room.
Furthermore, about 10 mL bacterial cultures of both S. aureus (Gram-positive) and E. coli (Gram-negative) that were already prepared were independently poured into the solid medium that was also already designed. While that, several sterilized Whatman filter paper discs of 1 cm in diameter were wetted with the fabricated ACNPs, AgNPs, and AgNPs-immobilized ACNPs each of 10% w/v for filter paper disc one; filter paper disc two, and filter disc three, respectively. In addition, the freshly prepared 40 ppm antibiotic amoxicillin solution and 0.5 mL distilled water were each dropped on the other sterilized Whatman filter papers of 1 cm in diameter as a positive and negative control, respectively. Furthermore, all of the treated Whatman filter paper discs aforementioned were put carefully on the bacterial cultures previously prepared with a certain distance to each other and incubated at 37 °C for 24 h in a microorganism-free room. The antibacterial effect of the ACNPs, AgNPs, and AgNPs-immobilized ACNPs materials was represented by appearing a clear zone around the associated paper disc after incubation time. The method of the aforementioned antibacterial test performed is illustrated (see Figure S1, Supporting Information).

2.8. Application of the ACNPs; AgNPs, and AgNPs-Immobilized on Polyester and Cotton Fabrics

This section was conducted to evaluate whether the enhanced antibacterial properties of AgNPs, namely AgNPs-immobilized ACNPs materials, could be utilized significantly to change the basic properties of polyester and cotton fabrics from non-antibacterial to be antibacterial in properties. It was performed as follows, some pieces of both 4 × 4 cm commercially available polyester and cotton fabrics were gently soaked independently in 50 mL aqueous colloidal AgNPs-immobilized ACNPs of 1%, 5%, and 10% w/v in concentration. These soaked fabrics were then agitated with ultrasonic energy of 40 kHz, 2 × 50 watt for 30 min at 50 °C temperature. After that, the materials were taken away from the colloidal and soaked again in the same manner. Only the surface of the fabrics was reversed in position. The fabrics were taken away from the colloidal, sprayed with distilled water until the black color of the washing water disappeared, and then dried at ambient conditions. Both virgin polyester and new cotton fabrics were also treated with the same materials and procedures for comparison.

3. Result and Discussion

3.1. Synthesis of AgNPs

The success of the synthesis of AgNPs from AgNO3 salt using aqueous aloe vera extract has been convincingly confirmed by UV-Vis’s spectrometer, as shown in Figure 1a. Meanwhile, Figure 1b shows its FTIR spectra, and Figure 1a shows appearing brown color demonstrated by the formation of the succeeding AgNPs, and in contrast, colorless AgNO3 solution as its precursor. It is similar to the AgNPs obtained by reference [8], which also exhibited brown color in an aqueous medium.
This phenomenon could be attributed to the correlation with the change of surface plasmon resonance (SPR) due to electron valence configuration change of Ag+ in AgNO3, i.e., [Kr] 4d10 (colorless) become Ag0 in AgNPs, i.e., [Kr] 4d105s1 (brown color). In this case, one electron in orbital s (spherical orbital) with the potential energy of level five leads to AgNPs that could absorb electromagnetic waves of visible light types, which is perceived as a brown color with human eyes.
The visible light energy would cause the electron to be excited from 5s orbital to the next 5p orbital (it might be either 5px, 5py, or 5pz). The minimal energy gap generated between 5s and 5p orbitals is precisely equal to the quantum energy of particular photons of visible light with longer wavelengths, revealing brown color. This situation does not occur in AgNO3 due to no electron existing in the 5s orbital of the associated Ag+ ion so that it exhibits colorless for our naked eyes perception. This phenomenon matches with the UV-Vis spectroscopy analysis of which AgNPs provided a wavelength peak at 480 nm, while AgNO3 at this wavelength did not generate a rise, as shown in Figure 1. The fact was slightly different from AgNPs produced by references [3,4], who reported that the UV-Vis maximum wavelength absorption of their AgNPs obtained was 405 nm, and 402–407 nm, respectively. However, our result was closer to AgNPs produced by reference [8] and reference [33]. They reported that their AgNPs exhibited a UV-Vis maximum absorption at 420 nm and 437 nm, respectively. The differences of AgNPs UV-Vis absorption peak location (SPR spectrum) and its spectrum broadness signified the various shape and sizes of the AgNPs produced [34,35]. In this case, the longer the UV-Vis wavelength absorbed signaling, the bigger the AgNPs produced in sizes. While the broadness of the SPR spectrum relates to the shape of the AgNPs, the broader range signals more non-spherical in the formation of the AgNPs produced [33,35]. In this context, a triangle shape AgNPs of 120 nm in maximum size provided a SPR peak at about 675 nm and generated a red color, a pentagon shape AgNPs of 105 in full size provided SPR peak at about 515 nm [35].
On the other hand, FTIR spectra of the synthesized AgNPs displayed in Figure 1b are similar to those reported by several references [36,37,38,39]. Figure 1b shows seven wave number peaks ranging from 4000 cm−1 to 500 cm−1. These are 3843; 3372; 2925; 1623; 1312; 1033; and 605 cm−1, each reflecting a stretching vibration of free atomic bond oxygen-hydrogen (O-H); bonded atomic bond O-H; unsaturated bond carbon-hydrogen (Csp2-H); double bond carbon-carbon (C=C); single bond nitrogen-oxygen (N-O); single bond carbon-oxygen (C-O); and single bond carbon-carbon (C-C), respectively [40,41,42,43,44] (see Table S1, Supporting Information).
All of the atomic vibration types aforementioned and that displayed (see Table S1, Supporting Information) attributed that the representative molecular structures of flavonoid, saponin, or tannin shown (see Figure S2, Supporting Information) are convincingly present in the aqueous aloe vera extract. In Figure 1b, the disappearing wavenumber peaks of about 1700 cm−1 suggested that the functional group of carbonyls (C=O) changed to hydroxyl (OH) one. It attributed tautomeric keto-enol in the molecule of myricetin occurred. The existence of such flavonoids, tannin, and saponin can stabilize the AgNPs formed core-shell nano or microstructures of AgNPs@flovonoid-saponin-tannin. The situation remarkably matches with its UV-Vis spectra at around 200 nm wavelength peaks, displayed in Figure 1a. This wavelength was signaling that electronic transition from both non-bonding electrons (n) as well as phi (π) bonding ones to antibonding phi (π*) of the associated secondary metabolites molecular structures happened.

3.2. Surface Morphology

Surface morphology and particle size distribution of the synthesized AgNPs, and the fabricated ACNPs explored with TEM is displayed in Figure 2. Figure 2a shows that a spherical particle cluster of about 5–10 nm in sizes (8.20 ± 2.05 nm in average size) of AgNPs could be appropriately separated from the other one of 18.80 ± 1.25 nm in average sizes when its aqueous medium was evaporated. In this case, the sizes of AgNPs particles could be approximately determined based on the associated bar marker. In addition, the standard deviation of the AgNPs particles size was calculated by employing an Excel-Microsoft Office software based on the particle’s sizes of mark i (20.0 nm), ii (20.0 nm), iii (19.0 nm), iv (17.5 nm), and v (17.5 nm). Meanwhile, Figure 2b shows three isolated AgNPs of which marked i, ii, and iii were about 5 nm, 10 nm, and 15 nm, respectively, with non-perfectly spherical shapes. This fact agrees with the UV-Vis spectrometer analysis, described in Section 3.1 in detail. Clustering the synthesized AgNPs of spherical and non-spherical shape also already happened, as shown in Figure 2a,b, respectively. The result is similar to that of many previous researchers whose publications have been reviewed in detail by reference [45].
Based on reference [45], the secondary metabolites of various plant extracts take a remarkable role in reducing silver ion (Ag+) to become silver metals (Ag0). Subsequently, the compounds assembling and stabilizing them form AgNPs with specific shapes and sizes [37,46]. It leads core-shell-like nanostructures of AgNPs secondary metabolites, which are confirmed so that the surface morphology of the synthesized AgNPs would vary depending on the typical molecular structure and composition of the associated secondary metabolites, which in our case is flavonoid, saponin, and tannin. The secondary metabolites most likely cause the synthesized AgNPs surface morphology to become rude.
On the other hand, Figure 2c,d displays the morphology and particle size distribution of the ACNPs explored with TEM. The spherical particles cluster of about 20.10 ± 1.52 in average sizes were joined to each other, some of them forming basic 2D nanostructures materials. In this case, ACNPs particles of circle mark i consisted of 5 particles of about 20, 20, 20, 18, and 18 nm each; that of circle mark ii consisted of 2 particles of about 20 and 20 nm each; and that of circle mark iii consisted of 3 particles of about 20, 22, and 23 nm each. Thus, by using an Excel-Microsoft Office software, the standard deviation of the ACNPs particle size was 1.52 nm. In addition, the stacking of other ACNPs particles of about 48.00 ± 8.37 nm in average size was also formed during the fabrication of ACNPs, as shown in Figure 2c marked with arrows. In contrast, some of the others include 3D nanostructure materials, as shown in Figure 2d(i,iii), respectively. Figure 2d shows other exciting facts. Adopting Lewis’s linear combination of atomic orbitals (LCAO) theory [47], this fact demonstrated that the spherical particles of around 20 nm in size could overlap each other to resemble atomic orbital overlapping shown by arrows (ii). It proved that such two particles’ adhesion energy is over their cohesion energy, and indicated the ACNPs particles present more on its surface rather than in its inside particles. This fact corresponded to the other research fact that observed the smaller the particles, as there were more atoms on its surface compared to its inside ones [48,49].
In conjunction to the FTIR spectra shown in Figure 1b, wavenumber peaks of the 2100 cm−1 to 1500 cm−1 range represented C=O of either carboxyl (-C=OOH), lactone (-OC=O) or ester (-C=OOR) group [18,36,38,39]. It indicated that acidic groups such as carboxyl, lactone, phenol, and basic groups such as chromene, ketones, and pyrones still exist in activated carbon produced under 600 °C heat treatment [22]. This situation causes the synthesized AgNPs to easier incorporate on the surface of the ACNPs when it has a core-shell nanostructure with such flavonoids, saponin, or tannin.
Furthermore, Figure 2e,f is TEM images of AgNPs-immobilized ACNPs. Despite AgNPs having already immobilized on the ACNPs surface, overlapping areas that connected two ACNPs to each other were still correctly occurring. It means functional groups that facilitate the connection are quite different in their properties and location. Figure 2f shows some black color dots on every ACNPs image’s surface. This phenomenon correlated to the flavonoid-saponin-tannin shell layer covering AgNPs, as shown in Figure 2g. It is attributed to the AgNPs that are chemically attached through hydrogen bonding with acidic functional groups (-COOH and -OH) present on the surface of ACNPs.

3.3. Antibacterial Activity Test

To evaluate the antibacterial activity of AgNPs and the influence of the ACNPs matrix on their antibacterial activity, we used E. coli and S. aureus bacteria of local strain, and the result was depicted (see Figure S3, Supporting Information). The antibacterial activity of the tested materials was evaluated according to the clear zone generated around the paper disks wetted by the associated materials tested. In this case, no clear zone generated around the distilled water-wetted paper disks (negative control), and contrary, the most expansive clear zone generated around the amoxicillin-wetted paper disks (positive control) in both E. coli (see Figure S3a, Supporting Information) and S. aureus (Figure S3b, Supporting Information). The wider the clear zone, the higher the antibacterial activity, and vice versa; the results are completely summarized in Table 1.
Using the broadest clear zone of 5.50 ± 0.00 mm in averages (positive control) on an E. coli-grown media as a reference to define the antibacterial properties of the materials tested, we can then propose a mathematical formula to state the antibacterial grade of the materials tested quantitively. The mathematical formula aforementioned is % α = (β ÷ γ) × 100% (antibacterial equation), where α, β, and γ each is the antibacterial activity, clear zone generated around the material tested, and the broadest clear zone generated around positive control, respectively. The antibacterial activity of amoxicillin towards E. coli was determined to be 100%, and that towards S. aureus was being 99.94% (~100%). This fact confirmed that amoxicillin is pure antibiotic medicine. However, the most important and interesting point was that the antibacterial activity of AgNPs material increased when immobilized on ACNPs, it was to E. coli as well as to S. aureus bacteria. In addition, Table 1 shows that the antibacterial activity of AgNPs increased considerably from 57.58% to 63.64% in E. coli (a rise of 6.06%); and that a more significant increase occurred in S. aureus when it was incorporated on the fabricated ACNPs materials, i.e., from 61.25% to 93.49% (increase 32.24%), and on the fabricated ACNPs materials, i.e., from 61.25% to 93.49% (increase 32.24%). It is convincing that the fabricated ACNPs can significantly increase the antibacterial activity of the synthesized AgNPs, especially towards E. coli, and S. aureus bacteria.
The fact can become a piece of additional evidence that AgNPs has a synergistic effect in antimicrobial activities, as reported by references [15,16,50]. Furthermore, the role of ACNPs in enhancing the antibacterial activity might be reasonably explained. Firstly, the AgNPs-immobilized ACNPs particles infiltrate into the bacteria cell wall and membrane, breaking down them due to the formation of hydrophilic bonding. It was facilitated by the bacterial cell wall that was commonly constructed by polymeric peptidoglycan, while the membrane has been built by various double layers phospholipids membrane [51,52,53]. The infiltration processes possibly took place since the particle sizes of AgNPs-immobilized ACNPs at just about 32.60 ± 4.88 nm in average size, based on Figure 2e,f. In this case, the standard deviation of the sizes of AgNPs-immobilized ACNPs particles determined by employing an Excel-Microsoft Office software based on the particle’s sizes mark i (35 nm), ii (30 nm), iii (28 nm), iv (30 nm), and v (40 nm) was about 4.88 nm. On the other hand, a bacterial cell is so much bigger than it—about more than 1000 nm. In addition, the infiltration process could be empowered by van der Waals interactions between the hydrophobic parts of the ACNPs and the bacterial cell wall and membrane.
The above actions lead to the cell wall as well as the membrane leaking and generate specific trajectories, which coincide with the AgNPs released from the ACNPs and subsequently kinetically through the cell wall and membrane entering the bacteria plasm. The entering process of AgNPs-immobilized ACNPs to the bacterial plasm aforementioned can be illustrated in Figure 3a,b. Using the surface morphology nomenclature proposed by reference [18], we used the Q1ST basin to propose the explanation of the antibacterial activity enhancement. In this explanation, certain cell organelles such as mitochondria, ribosomes, chromosomes, etc., of less than 20 nm in size were temporary deposited in the Q1ST basin generated on the ACNPs surface through hydrogen bonding, which occurred between the acidic groups and the organelles’ hydrophilic ones, e.g., ribosome, as illustrated in Figure 3c. Stretching and rocking vibrations of the hydrogen bonds then drove the bacterial ribosome to enter more deeply into the Q1ST basin until it reached the critical position, as illustrated in Figure 3d. In that position, the bacterial ribosome collapsed due to vibrational energy exposure coming from C=O, C-O, C-C, C=C, C-H, and O-H bonds which built up the Q1ST basin inner surface, including the hydrogen bonds located at the Q1ST gate, as illustrated in Figure 3e.
These processes were accompanied by releasing AgNPs from the hydrogen bonding compartments that may have existed on the surface of ACNPs. The bacteria-killing mechanism due to AgNPs itself has already been explained in detail by references [12,15,16,54,55,56].

3.4. Application of the Synthesized AgNPs on Textile Fabrics

The digital camera images of the AgNPs-immobilized ACNPs materials coated on the cotton and polyester fabrics is depicted in Figure 4. Figure 4 shows that the distances generated among the cotton fabrics’ strings were broader than those in the polyester fabrics. In addition, the number of holes generated among the yarns on the cotton fabric were lesser than that on the polyester fabrics. It demonstrated that the arrangement of the polyester yarns that constructed the polyester fabrics was denser than that of the cotton yarns.
Despite the yarns’ arrangements of the cotton fabrics were less dense than that of the polyester fabrics, the cotton fibers constructed the associated threads were dumber than that of the polyester, as shown in Figure 4c,f, respectively.
The two contradictive facts aforementioned could be a unique character for the fabrics, particularly when it was coated by antibacterial material, especially AgNPs-immobilized ACNPs. In this context, the combination of the AgNPs-immobilized ACNPs and ACNPs each coated on the cotton yarns turn out to provide antibacterial properties better than that coated on the polyester yarns. The arrangement of the associated yarns is shown in Figure 4a,b,d,e respectively. In these figures we can also see that many white dots are revealed on the polyester yarn rather than on cotton. The fact demonstrates that the combination materials of AgNPs-immobilized ACNPs and ACNPs could not be adequately attached to the polyester yarn compared to the cotton yarn.
Furthermore, the antibacterial activity of both cotton and polyester fabrics after they were coated with the AgNPs-immobilized ACNPs as well as with ACNPs each were depicted (see Figure S4, Supporting Information). In this case, a clear zone has been generated around several disks of cotton and polyester fabrics wetted with amoxicillin antibiotic (positive control), AgNPs-immobilized ACNPs, and ACNPs when all were incubated on both E. coli and S. aureus bacterial cultures. In contrast, the similar disk wetted with distilled water did not a generate clear zone at all when it was positioned on the same bacterial cultures. The width generated by the aforementioned clear zones is completely summarized in Table 2.
We can see in Table 2, the cotton fabric disk wetted with amoxicillin and positioned on the E. coli bacterial culture generated a clear zone with the widest radius, i.e., about 10.40 mm. Based on the broadest clear zone radius then, antibacterial activity of the fabrics was calculated through the antibacterial equation, Section 3.3. By this method, the associated materials-coated textile fabrics’ antibacterial activity could be expressed in a percentage value. We have calculated that the polyester fabric coated with AgNPs-immobilized ACNPs has a higher antibacterial activity towards E. coli and S. aureus bacteria, i.e., 31.73%, and 21.15%, than the cotton fabric covered with the same materials, i.e., 19.23%, and 13.36%, respectively. The facts indicate that the density of yarn was the more critical parameter in determining the antibacterial activity of the textile fabric than the density of fibers constructed in the associated thread.

Supplementary Materials

Schematic illustration of the antibacterial properties test of the fabricated ACNPs, AgNPs, and AgNPs-immobilized ACNPs (Figure S1); Representative molecular structure of (a) flavonoid [41], (b) saponin [42,43], and (c) tannin [44] (Figure S2); Antibacterial activity test towards (a) E. coli, (b) S. aureus. Here (1). Amoxicilin antibiotic (positive control), (2) Distilled water (negative control), (3). AgNPs-immobilized ACNPs; (4). AgNPs (Figure S3); Antibacterial activity test for cotton fabrics towards (a) E. coli; (b) S. aureus; and polyester fabrics towards (c) E. coli; (d) S. aureus. Here, (1). Wetted with amoxiciline (positive control); (2). Wetted with distilled water (negative control); (3). Wetted with AgNPs-incorporated ACNPs of 5% w/v in concentration; (4). Wetted with ACNPs of 5% w/v in concentration (Figure S4); Assignment of the synthesized AgNPs FTIR spectra, in detail (Table S1); Completely summary of the clear zone generated around the textile fabric disks, which wetted with the stated materials and positioned on E. coli and S. aureus’s bacterial culture (Table S2).

Author Contributions

Conceptualization, P.J.W., H.N.; methodology, P.J.W., M.A. and W.W.; validation, M.A. and W.W.; formal analysis: P.J.W., H.N., M.A. and W.W.; investigation, P.J.W. and M.A.; resources, P.J.W.; data curation: P.J.W.; writing—original draft preparation: P.J.W.; writing—review and editing: P.J.W., M.N., H.S. (Heri Sutanto), H.S. (Heru Susanto), and H.N.; supervision: H.N., M.N., H.S. (Heri Sutanto), and H.S. (Heru Susanto); funding acquisation: P.J.W. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Diponegoro University, grant number 276-41/UN7.5.1/PG/2017 and The APC was funded by Diponegoro University.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The relevant data of which is not displayed in this paper has been presented as Supporting Information file, and it can be accessed freely from the given link of Molecules-MDPI.

Acknowledgments

The authors are deeply grateful to Diponegoro University for financing this research through research grant number 276-41/UN7.5.1/PG/2017 of non APBN DPA SUKPA LPPM of the fiscal year 2017.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the colloidal activated carbon nanoparticles, and the synthesized colloidal silver nanoparticles are available from the authors.

References

  1. Burdusel, A.-C.; Gherasim, O.; Grumezescu, A.M.; Mogoanta, L.; Ficai, A.; Andronescu, E. Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials 2018, 8, 681. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Shao, Y.; Wu, C.; Wu, T.; Yuan, C.; Chen, S.; Ding, T.; Ye, X.; Hu, Y. Green synthesis of sodium alginate-silver nanoparticles and their antibacterial activity. Int. J. Biol. Macromol. 2018, 111, 1281–1292. [Google Scholar] [CrossRef] [PubMed]
  3. Sondi, I.; Sondi, B.S. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 2004, 275, 177–182. [Google Scholar] [CrossRef] [PubMed]
  4. Kraeling, M.E.K.; Topping, V.D.; Keltner, Z.M.; Belgrave, K.R.; Bailey, K.D.; Gao, X.; Yourick, J.J. In vitro percutaneous penetration of silver nanoparticles in pig and human skin. Regul. Toxicol. Pharmacol. 2018, 95, 314–322. [Google Scholar] [CrossRef] [PubMed]
  5. Gajda, K.D.; Nocun, M.; Roszak, J.; Janasik, B.; Quarles, C.D., Jr.; Wasowicz, W.; Grobelny, J.; Tomaszewska, E.; Celichowski, G.; Soliwoda, K.R.; et al. A study on the in vitro percutaneous absorption of silver nanoparticles in combination with aluminum chloride, methyl paraben or di-n-butyl phthalate. Toxicol. Lett. 2017, 272, 38–48. [Google Scholar] [CrossRef] [PubMed]
  6. Kumar, S.; Shukla, A.; Baul, P.P.; Mitra, A.; Halder, D. Biodegradable hybrid nanocomposites of chitosan/gelatin and silver nanoparticles for active food packaging applications. Food Packag. Shelf Life 2018, 16, 178–184. [Google Scholar] [CrossRef]
  7. Fortunati, E.; Peltzer, M.; Armentano, I.; Jiménez, A.; Kenny, J.M. Combined effects of cellulose nanocrystals and silver nanoparticles on the barrier and migration properties of PLA nano-biocomposites. J. Food Eng. 2013, 118, 117–124. [Google Scholar] [CrossRef]
  8. Tavaf, Z.; Tabatabaei, M.; Nezhad, A.K.; Panahi, F. Evaluation of antibacterial, antibiofilm, and antioxidant activities of synthesized silver nanoparticles (AgNPs) and casein peptide fragments against streptococcus mutans. Eur. J. Integr. Med. 2017, 12, 163–171. [Google Scholar] [CrossRef]
  9. Chowdhury, N.R.; MacGregor-Ramiasa, M.; Zilm, P.; Majewski, P.; Vasilev, K. Chocolate’ silver nanoparticles: Synthesis, antibacterial activity, and cytotoxicity. J. Colloid Interface Sci. 2016, 482, 151–158. [Google Scholar] [CrossRef] [PubMed]
  10. Zhou, Y.; Tang, R.C. Facile and eco-friendly fabrication of AgNPs coated silk for antibacterial and antioxidant textiles using honeysuckle extract. J. Photochem. Photobiol. B 2018, 178, 463–471. [Google Scholar] [CrossRef]
  11. Pannerselvam, B.; Jothinathan, M.K.D.; Rajendran, M.; Perumal, P.; Thangavelu, K.P.; Kim, H.J.; Singh, V.; Rangarajulu, S.K. An in vitro study on the burn wound healing activity of cotton fabrics immobilized with photosynthesized silver nanoparticles in male Wistar albino rats. Eur. J. Pharm. Sci. 2017, 100, 187–196. [Google Scholar] [CrossRef]
  12. Wang, Y.S.; Liu, L.; Fu, Q.; Sun, J.; An, Z.Y.; Ding, R.; Li, Y.; Zhao, X.D. Effect of Bacillus subtilis on corrosion behavior of 10MnNiCrCu steel in marine environment. Sci. Rep. 2020, 10, 5744. [Google Scholar] [CrossRef] [Green Version]
  13. Ishi, S.; Sadowsky, M.J. Escherichia coli in the Environment: Implications for Water Quality and Human Health. Microbes Environ. 2008, 23, 101–108. [Google Scholar] [CrossRef] [Green Version]
  14. Parham, S.; Chandren, S.; Wicaksono, D.H.B.; Bagherbaigi, S.; Lee, S.L.; Yuan, L.S.; Nur, H. Textile/Al2O3–TiO2 nanocomposite as an antimicrobial and radical scavenger wound dressing. R. Soc. Chem. Adv. 2016, 6, 8188–8197. [Google Scholar] [CrossRef]
  15. Yan, X.; He, B.; Liu, L.; Qu, G.; Shi, J.; Hu, L.; Jiang, G. Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: Proteomics approach. Metallomics 2018, 10, 557. [Google Scholar] [CrossRef]
  16. Durán, N.; Durán, M.; Jesus, M.B.; Seabra, A.B.; Fávaro, W.J.; Nakazato, G. Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity. Nanomed. Nanotechnol. Biol. Med. 2016, 12, 789–799. [Google Scholar] [CrossRef]
  17. Zhao, H.; Wang, Y.; Liu, Y.; Yin, K.; Wang, D.; Li, B.; Yu, H.; Xing, M. ROS-Induced Hepatotoxicity under Cypermethrin: Involvement of the Crosstalk between Nrf2/Keap1 and NF-κB/iκB-α Pathways Regulated by Proteasome. Environ. Sci. Technol. 2021, 55, 6171–6183. [Google Scholar] [CrossRef]
  18. Wibawa, P.J.; Nur, M.; Asy’ari, M.; Nur, H. SEM, XRD and FTIR analyses of both ultrasonic and heat generated activated carbon black microstructures. Heliyon 2020, 6, e03546. [Google Scholar] [CrossRef]
  19. Elkady, M.F.; Hussein, M.M.; Atiaa, H.M. Preparation of nano-activated carbon from carbon-based material for copper decontamination from wastewater. Am. J. Appl. Chem. 2015, 3, 31–37. [Google Scholar] [CrossRef] [Green Version]
  20. Haham, H.; Grinblat, J.; Sougrati, M.T.; Stievano, L.; Margel, S. Engineering of Iron-Based Magnetic Activated Carbon Fabrics for Environmental Remediation. Materials 2015, 8, 4593–4607. [Google Scholar] [CrossRef] [Green Version]
  21. Oliveira, L.C.A.; Rios, R.V.R.A.; Fabris, J.D.; Garg, V.; Sapag, K.; Lago, R.M. Activated carbon/ iron oxide magnetic composites for the adsorption of contaminants in water. Carbon 2002, 40, 2177–2183. [Google Scholar] [CrossRef]
  22. Tsai, W.-T.; Huang, P.-C.; Lin, Y.-Q. Reusing Cow Manure for the Production of Activated Carbon Using Potassium Hydroxide (KOH) Activation Process and Its Liquid-Phase Adsorption Performance. Processes 2019, 7, 737. [Google Scholar] [CrossRef] [Green Version]
  23. Shafeeyan, M.S.; Daud, W.M.A.W.; Houshmand, A.; Shamiri, A. A review on surface modification of activated carbon for carbon dioxide adsorption. J. Anal. Appl. Pyrolysis 2010, 89, 143–151. [Google Scholar] [CrossRef]
  24. Carmo, M.; Linardi, M.; Poco, J.G.R. H2O2 treated carbon black as electrocatalyst support for polymer electrolyte membrane fuel cell applications. Int. J. Hydrogen Energy 2008, 33, 6289–6297. [Google Scholar] [CrossRef]
  25. Knight, E.W.; Gillespie, A.K.; Prosniewski, M.J.; Stalla, D.; Dohnke, E.; Rash, T.A.; Pfeifer, P.; Wexler, C. Determination of the enthalpy of adsorption of hydrogen in activated carbon at room temperature. Int. J. Hydrogen Energy 2020, 45, 15541–15552. [Google Scholar] [CrossRef]
  26. Utrilla, J.R.; Díaz, J.M.; Polo, M.S.; García, M.A.F.; Toledo, I.B. Removal of the surfactant sodium dodecylbenzene sulphonate from water by the simultaneous use of ozone and powdered activated carbon: Comparison with systems based on O3 and O3/H2O2. Water Res. 2006, 40, 1717–1725. [Google Scholar] [CrossRef]
  27. Yang, X.; Wan, Y.; Zheng, Y.; He, F.; Yu, Z.; Huang, J.; Wang, H.; Ok, Y.S.; Jiang, Y.; Gao, B. Surface functional groups of carbon-based adsorbents and their roles in the removal of heavy metals from aqueous solutions: A critical review. Chem. Eng. J. 2019, 366, 608–621. [Google Scholar] [CrossRef]
  28. Nagornaya, M.N.; Razdyakonova, G.I.; Khodakova, S.Y. The effect of functional groups of carbon black on rubber properties: International Conference on Oil and Gas Engineering. Procedia Eng. 2016, 152, 563–569. [Google Scholar] [CrossRef] [Green Version]
  29. Harris, P.J.F.; Liu, Z.; Suenaga, K. Imaging the atomic structure of activated carbon. J. Phys. Condens. Matter 2008, 20, 36220. [Google Scholar] [CrossRef]
  30. Israelachvili, J.N. Van der Waals Forces between Particles and Surfaces. In Intermolecular and Surface Forces, 3rd ed.; Academic Press-Elsevier Inc.: Amsterdam, The Netherlands, 2011; pp. 253–289. ISBN 978-0-12-375182-9. [Google Scholar] [CrossRef]
  31. Lu, P.; Hsieh, Y.-L. Preparation and characterization of cellulose nanocrystals from rice straw. Carbohydr. Polym. 2012, 87, 564–573. [Google Scholar] [CrossRef]
  32. Harborne, J.B. Phytochemical Methods: A Guide to Modern Technique of Plant Analysis, 2nd ed.; Chapman and Hall: New York, NY, USA, 1984; pp. 1–221. ISBN 13 978-94-010-8956-2/978-94-009-5570-7. [Google Scholar] [CrossRef]
  33. Omrani, A.A.; Taghavinia, N. Photo-induced growth of silver nanoparticles using UV sensitivity of cellulose fibers. Appl. Surf. Sci. 2012, 258, 2373–2377. [Google Scholar] [CrossRef] [Green Version]
  34. Zhao, Y.; Jiang, Y.; Fang, Y. Spectroscopy property of Ag nanoparticles. Spectrochim. Acta Part A 2006, 65, 1003–1006. [Google Scholar] [CrossRef] [PubMed]
  35. Mock, J.J.; Barbic, M.; Smith, D.R.; Schultz, D.A.; Schultz, S. Shape effects in plasmon resonance of individual colloidal silver Nanoparticles. J. Chem. Phys. 2002, 116, 6755. [Google Scholar] [CrossRef]
  36. Hamouda, R.A.; Hussein, M.H.; Abo-elmagd, R.A.; Bawazir, S.S. Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Sci. Rep. 2019, 9, 13071. [Google Scholar] [CrossRef]
  37. Pirtarighat, S.; Ghannadnia, M.; Baghshahi, S. Green synthesis of silver nanoparticles using the plant extract of Salvia Spinosa grown in vitro and their antibacterial activity assessment. J. Nanostruct. Chem. 2019, 9, 1–9. [Google Scholar] [CrossRef] [Green Version]
  38. Jyoti, K.; Baunthiyal, M.; Singh, A. Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics. J. Radiat. Res. Appl. Sci. 2016, 9, 217–227. [Google Scholar] [CrossRef] [Green Version]
  39. Jyoti, K.; Singh, A. Green synthesis of nanostructured silver particles and their catalytic application in dye degradation. J. Genet. Eng. Biotechnol. 2016, 14, 311–317. [Google Scholar] [CrossRef] [Green Version]
  40. IUPAC: Commission on Molecular Structure and Spectroscopy: Tables of Wavenumbers for the Calibration of Infrared Spectrometers. International Union of Pure and Applied Chemistry (IUPAC). pp. 537–699. Available online: http://publications.iupac.org/pac/pdf/1960/pdf/0104x0537.pdf (accessed on 13 August 2019).
  41. Baranović, G.; Šegota, S. Infrared spectroscopy of flavones and flavonols. Reexamination of the hydroxyl and carbonyl vibrations in relation to the interactions of flavonoids with membrane lipids. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2018, 192, 473–486. [Google Scholar] [CrossRef]
  42. El Aziz, M.M.A.; Ashour, A.S.; Gomha Melad, A.S.G. A review on saponins from medicinal plants: Chemistry, isolation, and determination. J. Nanomed. Res. 2019, 7, 282–288. [Google Scholar] [CrossRef] [Green Version]
  43. Moghimipour, E.; Handali, S. Saponin: Properties, Methods of Evaluation and Applications. Annu. Res. Rev. Biol. 2015, 5, 207–220. [Google Scholar] [CrossRef] [Green Version]
  44. Okuda, T.; Ito, H. Tannins of Constant Structure in Medicinal and Food Plants-Hydrolyzable Tannins and Polyphenols Related tannins. Molecules 2011, 16, 2191–2217. [Google Scholar] [CrossRef]
  45. Rauwel, P.; Küünal, S.; Ferdov, S.; Erwan Rauwel, E. A Review on the Green Synthesis of Silver Nanoparticles and Their Morphologies Studied via TEM. Adv. Mater. Sci. Eng. 2015, 2015, 1–9. [Google Scholar] [CrossRef] [Green Version]
  46. Mousavi, S.M.; Hashemi, S.A.; Ghasemi, Y.; Atapour, A.; Amani, A.M.; Dashtaki, A.S.; Babapoor, A.; Arjmand, O. Green synthesis of silver nanoparticles toward bio and medical applications: Review study. Artif. Cells Nanomed. Biotechnol. 2018, 46, S855–S872. [Google Scholar] [CrossRef] [Green Version]
  47. House, J.E. Inorganic Chemistry: Covalent Bonding in Diatomic Molecules; Academic Press: Cambridge, MA, USA, 2008; pp. 76–78. [Google Scholar]
  48. Dowling, A. Nanoscience and Nanotechnology: Opportunities and Uncertainties; Royal Society/Royal Academy of Engineering: London, UK, 2005. [Google Scholar]
  49. Lead, J.R.; Wilkinson, K.J. Environmental Colloids and Particles: Current Knowledge and Future Development, Book Chapter I; Wiley: Chichester, UK, 2007; pp. 1–15. [Google Scholar]
  50. Garbin, R.P.B.; Otaguiri, E.S.; Morey, A.T.; da Silva, M.F.; Morguette, A.E.B.; Lancheros, C.A.C.; Kian, D.; Perugini, M.R.E.; Nakazato, G.; Durán, N.; et al. Effect of eugenol against Streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles. Evid. Based Complement. Altern. Med. 2015, 1–8. [Google Scholar] [CrossRef] [Green Version]
  51. Cardozo, V.F.; Oliveira, A.G.; Nishio, E.K.; Perugini, M.R.E.; Andrade, C.G.T.; Silveira, W.D.; Durán, N.; Andrade, G.; Kobayashi, R.K.T.; Nakazato, G. Antibacterial activity of extracellular compounds produced by a Pseudomonas strain against methicillin-resistant Staphylococcus aureus (MRSA) strains. Ann. Clin. Microbiol. Antimicrob. 2013, 12, 12. [Google Scholar] [CrossRef] [Green Version]
  52. Bogdanov, M.; Pyrshev, K.; Yesylevskyy, S.; Ryabichko, S.; Boiko, V.; Ivanchenko, P.; Kiyamova, R.; Guan, Z.; Ramseyer, C.; Dowhan, W. Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent. Sci. Adv. 2020, 6, eaaz6333. [Google Scholar] [CrossRef]
  53. Sohlenkamp, C.; Geiger, O. Review articles: Bacterial membrane lipids: Diversity in structures and pathways. FEMS Microbiol. Rev. 2016, 40, 133–159. [Google Scholar] [CrossRef] [Green Version]
  54. Clifton, L.A.; Skoda, M.W.A.; Daulton, E.L.; Hughes, A.V.; Le Brun, A.P.; Lakey, J.H.; Holt, S.A. Asymmetric phospholipid: Lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimic. J. R. Soc. Interface 2013, 10, 20130810. [Google Scholar] [CrossRef]
  55. Kedziora, A.; Speruda, M.; Krzyzewska, E.; Rybka, J.; Łukowiak, A.; Płoskonska, G.B. Similarities and Differences between Silver Ions and Silver in Nanoforms as Antibacterial Agents. Int. J. Mol. Sci. 2018, 19, 444. [Google Scholar] [CrossRef] [Green Version]
  56. Franci, G.; Falanga, A.; Galdiero, S.; Palomba, L.; Rai, M.; Morelli, G.; Galdiero, M. Silver Nanoparticles as Potential Antibacterial Agents. Molecules 2015, 20, 8856–8874. [Google Scholar] [CrossRef] [Green Version]
Molecules 26 03790 g001 550
Figure 1. (a) UV-Vis spectra of silver nitrate (AgNO3) solution and aqueous colloidal silver nanoparticles (AgNPs); (b) FTIR spectra of the synthesized AgNPs.
Figure 1. (a) UV-Vis spectra of silver nitrate (AgNO3) solution and aqueous colloidal silver nanoparticles (AgNPs); (b) FTIR spectra of the synthesized AgNPs.
Molecules 26 03790 g001
Molecules 26 03790 g002 550
Figure 2. (a,b) TEM images of AgNPs; (c,d) TEM image s of ACNPs; and (e,f) TEM images of AgNPs-immobilized ACNPs; (g) Schematic illustration of 5–10 nm AgNPs@Flovonoid-Saponin-Tannin core-shell nanostructures immobilized on the surface of 50 nm ACNPs. Here, the particles images marked (i), (ii), (iii), (iv), and (v) are randomly selected particles to determined the average size of the associated particles.
Figure 2. (a,b) TEM images of AgNPs; (c,d) TEM image s of ACNPs; and (e,f) TEM images of AgNPs-immobilized ACNPs; (g) Schematic illustration of 5–10 nm AgNPs@Flovonoid-Saponin-Tannin core-shell nanostructures immobilized on the surface of 50 nm ACNPs. Here, the particles images marked (i), (ii), (iii), (iv), and (v) are randomly selected particles to determined the average size of the associated particles.
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Molecules 26 03790 g003 550
Figure 3. Schematic illustration of (a,b) entering process of AgNPs-immobilized ACNPs to the bacterial cell plasm; (c–e) the role of ACNPs Q1ST turn in the synergistic effect of the synthesized AgNPs.
Figure 3. Schematic illustration of (a,b) entering process of AgNPs-immobilized ACNPs to the bacterial cell plasm; (c–e) the role of ACNPs Q1ST turn in the synergistic effect of the synthesized AgNPs.
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Molecules 26 03790 g004 550
Figure 4. The digital camera microscope images of the cotton fabrics coated with (a) AgNPs-immobilized ACNPs of 5% w/v concentration, (b) ACNPs of 5% w/v concentration_cotton, (c). Original cotton fabrics; and that of the polyester fabrics coated with (d) AgNPs-immobilized ACNPs of 5% w/v concentration, (e) ACNPs of 5% w/v concentration_polyester, (f) Original polyester fabrics. Here, 40× magnification digital camera was performed to (a,b,d,e); and 100× magnification digital camera was performed to (c,f).
Figure 4. The digital camera microscope images of the cotton fabrics coated with (a) AgNPs-immobilized ACNPs of 5% w/v concentration, (b) ACNPs of 5% w/v concentration_cotton, (c). Original cotton fabrics; and that of the polyester fabrics coated with (d) AgNPs-immobilized ACNPs of 5% w/v concentration, (e) ACNPs of 5% w/v concentration_polyester, (f) Original polyester fabrics. Here, 40× magnification digital camera was performed to (a,b,d,e); and 100× magnification digital camera was performed to (c,f).
Molecules 26 03790 g004
Table
Table 1. Summary of the clear zone width generated around the paper disks wetted with the materials tested regarding their antibacterial activity.
Table 1. Summary of the clear zone width generated around the paper disks wetted with the materials tested regarding their antibacterial activity.
Materials, 10% in ConcentrationClear Zone Radius/mm
E. coliS. aureus
IIIIIIAverage% Antibacterial Activity *IIIIIIAverage% Antibacterial Activity *
AgNPs2.504.502.503.17 ± 1.1557.583.503.502.503.17 ± 0.5861.25
AgNPs-immobilized ACNPs 3.503.503.503.50 ± 0.0063.645.504.504.504.83 ± 0.5093.49
Amoxicillin (positive control)5.505.505.505.50 ± 0.00100.004.505.505.505.17 ± 0.5899.94
Distilled water (negative control)0000000000
* % Antibacterial activity = (Clear zone generated around the material tested ÷ The most expansive clear area developed around positive control) × 100%.
Table
Table 2. Summary of the clear zone generated around the textile fabric disks, which wetted with the stated materials and positioned on E. coli and S. aureus’s bacterial culture.
Table 2. Summary of the clear zone generated around the textile fabric disks, which wetted with the stated materials and positioned on E. coli and S. aureus’s bacterial culture.
Textile Fabrics Wetted with Materials of 5% w/v in Concentration *Clear Zone
E. coliS. aureus
Radius/mm% Antibacterial Activity **Radius/mm% Antibacterial Activity **
CottonACNPs0000
AgNPs-immobilized ACNPs2.0019.231.4013.36
Amoxicillin (positive control)10.401008.5081.73
Distilled water (negative control0000
PolyesterACNPs0000
AgNPs-immobilized ACNPs3.3031.732.2021.15
Amoxicillin (positive control)8.3079.817.9075.96
Distilled water (negative control0000
* See Table S2, Supporting Information for the concentration of 1% and 10% w/v. ** % Antibacterial activity = (Clear zone generated around the material tested ÷ The most expansive clear area developed around positive control) × 100%.
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Wibawa, P.J.; Nur, M.; Asy’ari, M.; Wijanarka, W.; Susanto, H.; Sutanto, H.; Nur, H. Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics. Molecules 2021, 26, 3790. https://doi.org/10.3390/molecules26133790

AMA Style

Wibawa PJ, Nur M, Asy’ari M, Wijanarka W, Susanto H, Sutanto H, Nur H. Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics. Molecules. 2021; 26(13):3790. https://doi.org/10.3390/molecules26133790

Chicago/Turabian Style

Wibawa, Pratama Jujur, Muhammad Nur, Mukhammad Asy’ari, Wijanarka Wijanarka, Heru Susanto, Heri Sutanto, and Hadi Nur. 2021. "Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics" Molecules 26, no. 13: 3790. https://doi.org/10.3390/molecules26133790

APA Style

Wibawa, P. J., Nur, M., Asy’ari, M., Wijanarka, W., Susanto, H., Sutanto, H., & Nur, H. (2021). Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics. Molecules, 26(13), 3790. https://doi.org/10.3390/molecules26133790

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