Anticancer Activity of 2-O-caffeoyl Alphitolic Acid Extracted from the Lichen, Usnea barbata 2017-KL-10
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structure Identification of Compounds 1–8
2.2. 2-O-Caffeoyl Alphitolic Acid Inhibits HCT116 Cell Growth
2.3. 2-O-Caffeoyl Alphitolic Acid Enhances Cell Apoptosis
2.4. 2-O-Caffeoyl Alphitolic Acid Inhibits Cell Migration and Invasion
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Lichen Material
3.3. Extraction and Isolation
3.4. Cell Culture and Cell Proliferation Assay
3.5. Apoptotic Analysis
3.6. RT-PCR for Apoptosis-Associated Genes
3.7. Wound-Healing Assay
3.8. Migration and Invasion Assay
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Chae, H.-J.; Kim, G.-J.; Deshar, B.; Kim, H.-J.; Shin, M.-J.; Kwon, H.; Youn, U.-J.; Nam, J.-W.; Kim, S.-H.; Choi, H.; et al. Anticancer Activity of 2-O-caffeoyl Alphitolic Acid Extracted from the Lichen, Usnea barbata 2017-KL-10. Molecules 2021, 26, 3937. https://doi.org/10.3390/molecules26133937
Chae H-J, Kim G-J, Deshar B, Kim H-J, Shin M-J, Kwon H, Youn U-J, Nam J-W, Kim S-H, Choi H, et al. Anticancer Activity of 2-O-caffeoyl Alphitolic Acid Extracted from the Lichen, Usnea barbata 2017-KL-10. Molecules. 2021; 26(13):3937. https://doi.org/10.3390/molecules26133937
Chicago/Turabian StyleChae, Hae-Jung, Geum-Jin Kim, Barsha Deshar, Hyun-Jin Kim, Min-Ji Shin, Hyukbean Kwon, Ui-Joung Youn, Joo-Won Nam, Sung-Hak Kim, Hyukjae Choi, and et al. 2021. "Anticancer Activity of 2-O-caffeoyl Alphitolic Acid Extracted from the Lichen, Usnea barbata 2017-KL-10" Molecules 26, no. 13: 3937. https://doi.org/10.3390/molecules26133937