The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mesocosms
2.2. DNT Concentration
2.3. Microbial Community Analysis (16S DNA Illumina MiSeq Sequencing)
2.4. Quantification of Total Fungi, Bacteria (qPCR)
2.5. Data Analysis
3. Results and Discussion
3.1. DNT Remediation
3.2. Total Microbial Community Analysis Quantitative PCR
3.3. Bacterial Community Diversity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Sludge Elements/Parameters | Units | Value |
---|---|---|
pH | 9.2 | |
Organic C | % | 7.7 |
Colour | Yellow | |
Nitrite | mg/kg | 1900 |
Nitrate | mg/kg | 7200 |
Sulphate | mg/kg | 6700 |
Moisture | % | 41 |
2,4,6 TNT | mg/kg | <50 |
2,4 DNT | g/L | 300 |
2,6 DNT | g/L | 0.056 |
RDX | <50 |
Treatments | Symbol | Nutrient Formulation | Hydrocarbonoclastic Bacteria |
---|---|---|---|
Natural attenuation (control) | NA | - | None |
Bioaugmentation 1 | BA | - | Bacilli consortium |
Bioaugmentation 2 | ST | - | Streptomyces sp. |
Bioaugmentation 3 | FS | - | Fusarium solani |
Biostimulation | BS | + | None |
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Aburto-Medina, A.; Shahsavari, E.; Taha, M.; Bates, A.; Van Ieperen, L.; Ball, A.S. The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge. Molecules 2021, 26, 4814. https://doi.org/10.3390/molecules26164814
Aburto-Medina A, Shahsavari E, Taha M, Bates A, Van Ieperen L, Ball AS. The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge. Molecules. 2021; 26(16):4814. https://doi.org/10.3390/molecules26164814
Chicago/Turabian StyleAburto-Medina, Arturo, Esmaeil Shahsavari, Mohamed Taha, Andrew Bates, Leon Van Ieperen, and Andrew S. Ball. 2021. "The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge" Molecules 26, no. 16: 4814. https://doi.org/10.3390/molecules26164814