Chitosan (CTS) Alleviates Heat-Induced Leaf Senescence in Creeping Bentgrass by Regulating Chlorophyll Metabolism, Antioxidant Defense, and the Heat Shock Pathway
Abstract
:1. Introduction
2. Results
2.1. Effects of the CTS on Water Status, Photochemical Efficiency, and Chl Metabolism
2.2. Effects of the CTS on Oxidative Damage and Antioxidant Enzyme Activities
2.3. Effects of the CTS on Expression Level of Genes Involved in HSF Pathway and Senescence
3. Discussion
4. Materials and Methods
4.1. Plant Material and Treatment
4.2. Determination of Water Status and Photosynthetic Parameters
4.3. Determination of Oxidative Damage and Antioxidant Enzyme Activities
4.4. Genes Expression Analyses
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Target Gene | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Tm (°C) |
---|---|---|---|
β-Actin | CCTTTTCCAGCCATCTTTCA | GAGGTCCTTCCTGATATCCA | 58 |
AsPBGD | TAGCGCTGCGGATTAGAACT | GAAGGATAACGAACCGCTGA | 55 |
AsCHLH | CATCAGGGCGGATAGAGAGA | TCTGCCACAATCAGCTTCAG | 56 |
AsPPH | GAATGTCATTGCCGTCTGAA | CAATGAAATGCTGGACCTGA | 53 |
AsHSFA-6a | CACCTTCGAGGAGCTGGCATTG | TGTCTATCTCCGCCTGCTCATCC | 62 |
AsHSP82 | GAGCCTGACGGACAAGAGCAAG | GGAGTGAAGCAGAGTAACGAGACG | 64 |
AsSAG12 | CCCAGCAGTTTACTGGCTTT | AAGCAGGTGCCTTGAAACTT | 54 |
AsSAG39 | CCTCGCTGTTCTTGCCGTGAG | CGTGCTCAGCCATCCACTTCTC | 63 |
Asl20 | GGGTAGACGGCAACGATACT | TACTTGGTTGAATCGTCGGA | 60 |
Ash36 | TGGGAATGTGTTCAGGGTAA | TCACCTCGATGAGGTAGTCG | 55 |
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Huang, C.; Tian, Y.; Zhang, B.; Hassan, M.J.; Li, Z.; Zhu, Y. Chitosan (CTS) Alleviates Heat-Induced Leaf Senescence in Creeping Bentgrass by Regulating Chlorophyll Metabolism, Antioxidant Defense, and the Heat Shock Pathway. Molecules 2021, 26, 5337. https://doi.org/10.3390/molecules26175337
Huang C, Tian Y, Zhang B, Hassan MJ, Li Z, Zhu Y. Chitosan (CTS) Alleviates Heat-Induced Leaf Senescence in Creeping Bentgrass by Regulating Chlorophyll Metabolism, Antioxidant Defense, and the Heat Shock Pathway. Molecules. 2021; 26(17):5337. https://doi.org/10.3390/molecules26175337
Chicago/Turabian StyleHuang, Cheng, Yulong Tian, Bingbing Zhang, Muhammad Jawad Hassan, Zhou Li, and Yongqun Zhu. 2021. "Chitosan (CTS) Alleviates Heat-Induced Leaf Senescence in Creeping Bentgrass by Regulating Chlorophyll Metabolism, Antioxidant Defense, and the Heat Shock Pathway" Molecules 26, no. 17: 5337. https://doi.org/10.3390/molecules26175337