Development of CE-C4D Method for Determination Tropane Alkaloids
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Separation Conditions
2.1.1. Selection of the Background Electrolyte
2.1.2. Instrumental Separation Conditions
2.2. Method Validation
2.3. Analysis of Solanaceae Plant Samples
2.4. Analysis of Pharmaceuticals
2.5. Assesment of the Optimized Method with RGB Model
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. Preparation of Stock and Standard Solutions
3.3. Extraction Protocol
3.4. Instrumentation
3.5. Data Treatment
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Sample ID 1 | Atropine 2 | Scopolamine 2 |
---|---|---|
DM1 | 3994 ± 49 | 2637 ± 26 |
DM2 | 4270 ± 20 | 2677 ± 16 |
HN1 | 609.6 ± 21.7 | 251.2 ± 14.3 |
HN2 | 602.4 ± 13.4 | 214.8 ± 13.7 |
DS1 | 985.5 ± 13.1 | <LOQ |
DS2 | 1096 ± 13 | <LOQ |
DA1 | 467.3 ± 3.0 | 1827 ± 26 |
DA2 | 521.8 ± 4.5 | 2080 ± 22 |
AB1 | 8906 ± 20 | ND |
AB2 | 9810 ± 83 | ND |
SL1 | 3004 ± 64 | 1085 ± 17 |
SL2 | 3135 ± 60 | 1122 ± 26 |
Parameter | Atropine | Scopolamine |
---|---|---|
Linearity (μg/mL) | 5–50 | 5–50 |
Slope | 0.027 | 0.032 |
Intercept | −0.034 | −0.023 |
R-squared | 0.999 | 0.996 |
LOD (μg/mL) | 0.5 | 0.5 |
LOQ (μg/mL) | 1.5 | 1.5 |
Precision 1, CV (%): | ||
5 μg/mL | 1.88 | 1.66 |
20 μg/mL | 2.08 | 1.07 |
50 μg/mL | 1.18 | 1.73 |
Intermediate precision 1, CV (%): | ||
5 μg/mL | 1.69 | 2.28 |
20 μg/mL | 2.13 | 2.77 |
50 μg/mL | 1.16 | 1.59 |
Recovery, RE (%): | ||
+12.5 μg/mL | 102.7 | 101.0 |
+25 μg/mL | 101.3 | 101.8 |
Matrix effect, ME (%): | ||
+12.5 μg/mL | 93.56 | 92.99 |
+25 μg/mL | 94.10 | 94.23 |
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Gołąb, M.; Przybyłowska, M.; Kubáň, P.; Itterheimová, P.; Woźniakiewicz, M. Development of CE-C4D Method for Determination Tropane Alkaloids. Molecules 2021, 26, 5749. https://doi.org/10.3390/molecules26195749
Gołąb M, Przybyłowska M, Kubáň P, Itterheimová P, Woźniakiewicz M. Development of CE-C4D Method for Determination Tropane Alkaloids. Molecules. 2021; 26(19):5749. https://doi.org/10.3390/molecules26195749
Chicago/Turabian StyleGołąb, Małgorzata, Martyna Przybyłowska, Petr Kubáň, Petra Itterheimová, and Michał Woźniakiewicz. 2021. "Development of CE-C4D Method for Determination Tropane Alkaloids" Molecules 26, no. 19: 5749. https://doi.org/10.3390/molecules26195749