Falcarindiol Isolated from Notopterygium incisum Inhibits the Quorum Sensing of Pseudomonas aeruginosa
Abstract
:1. Introduction
2. Results
2.1. Screening and Identification of Falcaridiol
2.2. Effect of Falcarindiol on QS-Related Virulence Phenotype
2.3. Effect of Falcarindiol on Expression of QS-Regulated Genes
2.4. The Inhibition Effect of Falcarindiol on QS System
2.5. Effect of Falcarindiol on the Binding of LasR to the Promoter Regions
2.6. Molecular Docking Analysis
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Plasmids, and Materials
4.2. Screening of QSIs
4.3. Purification of Active Compound
4.4. Growth Curve Assay
4.5. Agar Well-Diffusion Assay
4.6. Analysis of lasB, pqsA, and rhlA Transcriptional Activity in E. coli
4.7. Virulence Factor Assays
4.8. Biofilm Formation Assay
4.9. Real-Time RT-PCR Assay
4.10. EMSA
4.11. Molecular Docking Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Zhao, C.; Zheng, H.; Zhou, L.; Ji, H.; Zhao, L.; Yu, W.; Gong, Q. Falcarindiol Isolated from Notopterygium incisum Inhibits the Quorum Sensing of Pseudomonas aeruginosa. Molecules 2021, 26, 5896. https://doi.org/10.3390/molecules26195896
Zhao C, Zheng H, Zhou L, Ji H, Zhao L, Yu W, Gong Q. Falcarindiol Isolated from Notopterygium incisum Inhibits the Quorum Sensing of Pseudomonas aeruginosa. Molecules. 2021; 26(19):5896. https://doi.org/10.3390/molecules26195896
Chicago/Turabian StyleZhao, Chaoyue, Hongda Zheng, Liman Zhou, Hongrui Ji, Lu Zhao, Wengong Yu, and Qianhong Gong. 2021. "Falcarindiol Isolated from Notopterygium incisum Inhibits the Quorum Sensing of Pseudomonas aeruginosa" Molecules 26, no. 19: 5896. https://doi.org/10.3390/molecules26195896