Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection
Abstract
:1. Introduction
2. Results
2.1. Structure Characterization
2.2. Metal Ion Selectivity
2.3. Visual Detection of Copper Ions
2.4. Quantitative Detection of Copper Ions
2.5. Influences of pH and Time on the Detection of Copper Ions
2.6. MTT Assay
2.7. Recognition Mechanism
2.8. Bioimaging
3. Discussion
4. Materials and Methods
4.1. Materials and Instrumentation
4.2. Synthesis and Characterization of Probes J6/J7
4.2.1. 6-Hydroxyl-4-Methylcoumarin (Scheme 1a)
4.2.2. 5-Formyl-6-Hydroxyl-4-Methylcoumarin (Scheme 1b)
4.2.3. Rhodamine 6G Acylhydrazine (Scheme 1c)
4.2.4. Probe J6
4.2.5. Probe J7
4.3. Spectroscopic Analysis
4.4. Cytotoxicity
4.5. Fluorescent Imaging in Living Cells
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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He, H.; Cheng, Z.; Zheng, L.; Zhang, X. Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection. Molecules 2021, 26, 512. https://doi.org/10.3390/molecules26020512
He H, Cheng Z, Zheng L, Zhang X. Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection. Molecules. 2021; 26(2):512. https://doi.org/10.3390/molecules26020512
Chicago/Turabian StyleHe, Hao, Zhao Cheng, Lei Zheng, and Xuejiao Zhang. 2021. "Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection" Molecules 26, no. 2: 512. https://doi.org/10.3390/molecules26020512