Chemical Elicitors-Induced Variation in Cellular Biomass, Biosynthesis of Secondary Cell Products, and Antioxidant System in Callus Cultures of Fagonia indica
Abstract
:1. Introduction
2. Results and Discussion
2.1. Abiotic Elicitors-Induced Variation in Cellular Biomass in Calli Cultures of F. indica
2.2. Effect of Elicitation on Total Phenolic and Flavonoid Biosynthesis
2.3. Correlation of Total Phenolics and Flavonoids Content with Radical Scavenging Activity
2.4. Effect of AlCl3 and CdCl2 on Antioxidant Enzyme Activities
2.5. Quantification of the Main Phytochemicalsin-Treated Callus Cultures of F. indica
3. Materials and Methods
3.1. Elicitation of Callus Culture with AlCl3 and CdCl2
3.2. Determination of Total Phenolic Content (TPC) and Flavonoid Content (TFC)
3.3. Determination of SOD and POD Activities
3.4. Determination of Free Radical Scavenging Assay (DPPH)
3.5. HPLC Quantification
3.6. Experimental Design and Data Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Compounds Quantified (µg/mg-DW) | 0.01 mM | 0.05 mM | 0.1 mM | 0.5 mM | 1.0 mM | 2.0 mM | 5.0 mM | 0mM | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AlCl3 | CdCl2 | AlCl3 | CdCl2 | AlCl3 | CdCl2 | AlCl3 | CdCl2 | AlCl3 | CdCl2 | AlCl3 | CdCl2 | AlCl3 | CdCl2 | Control | |
GALLIC ACID | 0.062 ± 0.022 | 0.061 ± 0.005 | 0.055 ± 0.001 | 0.055 ± 0.001 | 0.056 ± 003 | 0.062 ± 0.001 | 0.056 ± 0.017 | 0.044 ± 0.0012 | 0.057 ± 0.023 | 0.035 ± 0.001 | 0.054 ± 0.009 | 0.033 ± 0.019 | 0.051 ± 0.008 | 0.033 ± 0.022 | 0.053 ± 0.011 |
CAFFEIC ACID | 0.097 ± 0.012 | 0.094 ± 0.008 | 0.082 ± 0.002 | 0.081 ± 0.007 | 0.084 ± 0.010 | 0.095 ± 0.001 | 0.083 ± 0.0057 | 0.059 ± 0.0064 | 0.086 ± 0.028 | 0.040 ± 0.032 | 0.080 ± 0.011 | 0.037 ± 0.020 | 0.073 ± 0.012 | 0.037 ± 0.033 | 0.077 ± 0.081 |
MYRICETIN | 0.655 ± 0.014 | 0.799 ± 0.023 | 1.199 ± 0.020 | 0.609 ± 0.013 | 0.827 ± 0.019 | 0.771 ± 0.002 | 1.129 ± 0.017 | 0.777 ± 0.0058 | 0.662 ± 0.034 | 0.725 ± 0.009 | 0.831 ± 0.072 | 0.583 ± 0.076 | 0.980 ± 0.015 | 0.796 ± 0.051 | 0.702 ± 0.021 |
CATECHIN | 0.522 ± 0.09 | 0.476 ± 0.013 | 0.547 ± 0.013 | 0.415 ± 0.009 | 0.623 ± 0.023 | 0.444 ± 0.0023 | 0.533 ± 0.021 | 0.566 ± 0.0031 | 0.589 ± 0.017 | 0.560 ± 0.051 | 0.588 ± 0.016 | 0.443 ± 0.012 | 0.632 ± 0.018 | 0.464 ± 0.060 | 0.578 ± 0.025 |
KAEMPHEROL | 0.560 ± 0.016 | 0.476 ± 0.019 | 0.509 ± 0.017 | 0.574 ± 0.019 | 0.456 ± 0.021 | 0.481 ± 0.0038 | 0.605 ± 0.0057 | 0.528 ± 0.0018 | 0.626 ± 0.011 | 0.523 ± 0.068 | 0.644 ± 0.026 | 0.530 ± 0.069 | 0.595 ± 0.028 | 0.551 ± 0.062 | 0.435 ± 0.013 |
ISORHAMNETIN | 0.674 ± 0.08 | 0.422 ± 0.011 | 0.701 ± 0.018 | 0.486 ± 0.008 | 0.567 ± 0.031 | 0.408 ± 0.0013 | 0.641 ± 0.0036 | 0.662 ± 0.0017 | 0.499 ± 0.026 | 0.670 ± 0.015 | 0.569 ± 0.029 | 0.513 ± 0.072 | 0.522 ± 0.044 | 0.568 ± 0.013 | 0.524 ± 0.012 |
APIGENIN | 0.883 ± 0.010 | 0.601 ± 0.015 | 0.720 ± 0.013 | 0.574 ± 0.012 | 0.660 ± 0.002 | 0.626 ± 0.0028 | 0.804 ± 0.011 | 0.681 ± 0.0015 | 0.626 ± 0.034 | 0.670 ± 0.018 | 0.737 ± 0.022 | 0.739 ± 0.021 | 0.632 ± 0.065 | 0.691 ± 0.016 | 0.524 ± 0.033 |
NAHAGENIN | 0.142 ± 0.020 | 0.146 ± 0.029 | 0.110 ± 0.001 | 0.121 ± 0.001 | 0.118 ± 0.005 | 0.145 ± 0.0023 | 0.120 ± 0.024 | 0.065 ± 0.0019 | 0.127 ± 0.051 | 0.031 ± 0.027 | 0.110 ± 0.038 | 0.028 ± 0.016 | 0.098 ± 0.011 | 0.028 ± 0.019 | 0.112 ± 0.018 |
HEDERAGENIN | 0.028 ± 0.017 | 0.028 ± 0.002 | 0.024 ± 0.004 | 0.025 ± 0.003 | 0.025 ± 0.009 | 0.028 ± 0.0015 | 0.025 ± 0.023 | 0.018 ± 0.0040 | 0.026 ± 0.011 | 0.013 ± 0.025 | 0.024 ± 0.041 | 0.012 ± 0.011 | 0.022 ± 0.010 | 0.012 ± 0.028 | 0.024 ± 0.041 |
URSOLIC ACID | 0.201 ± 0.001 | 0.114 ± 0.010 | 0.143 ± 0.025 | 0.122 ± 0.007 | 0.148 ± 0.011 | 0.135 ± 0.0017 | 0.134 ± 0.031 | 0.173 ± 0.0034 | 0.087 ± 0.026 | 0.195 ± 0.022 | 0.139 ± 0.034 | 0.203 ± 0.052 | 0.155 ± 0.021 | 0.130 ± 0.035 | 0.094 ± 0.030 |
BETULINIC ACID | 0.338 ± 0.005 | 0.349 ± 0.003 | 0.260 ± 0.003 | 0.288 ± 0.006 | 0.280 ± 0.009 | 0.348 ± 0.0011 | 0.286 ± 0.0057 | 0.146 ± 0.0011 | 0.304 ± 0.0173 | 0.061 ± 0.020 | 0.259 ± 0.033 | 0.056 ± 0.038 | 0.230 ± 0.049 | 0.054 ± 0.011 | 0.266 ± 0.022 |
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Khan, H.; Khan, T.; Ahmad, N.; Zaman, G.; Khan, T.; Ahmad, W.; Batool, S.; Hussain, Z.; Drouet, S.; Hano, C.; et al. Chemical Elicitors-Induced Variation in Cellular Biomass, Biosynthesis of Secondary Cell Products, and Antioxidant System in Callus Cultures of Fagonia indica. Molecules 2021, 26, 6340. https://doi.org/10.3390/molecules26216340
Khan H, Khan T, Ahmad N, Zaman G, Khan T, Ahmad W, Batool S, Hussain Z, Drouet S, Hano C, et al. Chemical Elicitors-Induced Variation in Cellular Biomass, Biosynthesis of Secondary Cell Products, and Antioxidant System in Callus Cultures of Fagonia indica. Molecules. 2021; 26(21):6340. https://doi.org/10.3390/molecules26216340
Chicago/Turabian StyleKhan, Habiba, Tariq Khan, Nisar Ahmad, Gouhar Zaman, Taimoor Khan, Waqar Ahmad, Sannia Batool, Zahid Hussain, Samantha Drouet, Christophe Hano, and et al. 2021. "Chemical Elicitors-Induced Variation in Cellular Biomass, Biosynthesis of Secondary Cell Products, and Antioxidant System in Callus Cultures of Fagonia indica" Molecules 26, no. 21: 6340. https://doi.org/10.3390/molecules26216340