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Review

Review: Radionuclide Molecular Imaging Targeting HER2 in Breast Cancer with a Focus on Molecular Probes into Clinical Trials and Small Peptides

by
Shushan Ge
1,2,†,
Jihui Li
1,†,
Yu Yu
1,†,
Zhengguo Chen
2,
Yi Yang
3,
Liqing Zhu
2,
Shibiao Sang
1,* and
Shengming Deng
1,2,4,*
1
Department of Nuclear Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
2
Nuclear Medicine Laboratory of Mianyang Central Hospital, Mianyang 621099, China
3
Department of Nuclear Medicine, The Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University, Suzhou 215163, China
4
State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2021, 26(21), 6482; https://doi.org/10.3390/molecules26216482
Submission received: 20 September 2021 / Revised: 18 October 2021 / Accepted: 26 October 2021 / Published: 27 October 2021
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Abstract

:
As the most frequently occurring cancer worldwide, breast cancer (BC) is the leading cause of cancer-related death in women. The overexpression of HER2 (human epidermal growth factor receptor 2) is found in about 15% of BC patients, and it is often associated with a poor prognosis due to the effect on cell proliferation, migration, invasion, and survival. As a result of the heterogeneity of BC, molecular imaging with HER2 probes can non-invasively, in real time, and quantitatively reflect the expression status of HER2 in tumors. This will provide a new approach for patients to choose treatment options and monitor treatment response. Furthermore, radionuclide molecular imaging has the potential of repetitive measurements, and it can help solve the problem of heterogeneous expression and conversion of HER2 status during disease progression or treatment. Different imaging probes of targeting proteins, such as monoclonal antibodies, antibody fragments, nanobodies, and affibodies, are currently in preclinical and clinical development. Moreover, in recent years, HER2-specific peptides have been widely developed for molecular imaging techniques for HER2-positive cancers. This article summarized different types of molecular probes targeting HER2 used in current clinical applications and the developmental trend of some HER2-specific peptides.

1. Introduction

Breast cancer (BC) has surpassed lung cancer as the most frequently occurring cancer worldwide, with 2.3 million new cases diagnosed in 2020 (11.7% of all cancers), and BC ranks the fifth cause of cancer-related death overall and the leading cause of cancer death in women [1]. BC is a highly heterogeneous type of tumor [2], and it can be mainly categorized into four different subtypes: luminal A, luminal B, HER2 (human epidermal growth factor receptor 2)-positive, and triple-negative [3]. Currently, estrogen receptor (ER), progesterone receptor (PR), and HER2 are three main clinical therapeutic targets for BC [2,4,5]. The overexpression of HER2 is found in about 15% of BC patients [6]. Furthermore, the overexpression of HER2 is often associated with a poor prognosis due to the effect on cell proliferation, migration, invasion, and survival [7,8,9].
Targeting HER2 with a range of anti-HER2 drugs has been successfully applied in clinical practice and significantly improved patient outcomes in both advanced and early disease settings [10]. However, there is increasing evidence of temporal and spatial heterogeneity in BC HER2 overexpression [11,12,13]. About 10.3% of patients will have inconsistent HER2 expression between primary breast tumors and distant metastases [13]. Therefore, accurate assessment of HER2 expression plays an essential role in cancer diagnosis and treatment.
The biopsy-based diagnosis has been proved to be clinically useful [11]. However, it is difficult to determine the heterogeneity and status discordance between primary and metastatic tissues using biopsy [14]. Moreover, bone metastases are frequently detected in BC patients, and it is difficult to access their status by using biopsy [15]. Compared with biopsy, molecular imaging with anti-HER2 probes allows the non-invasive, whole-body assessment of tumor burden and may enable the selection of patients for HER2-targeted therapy, dosage optimization, schedule of treatment, and assessment of response to anti-HER2 therapies [16,17,18,19]. This approach can reveal intertumoral or intratumoral heterogeneity as well as variations in HER2 expression over time.
In the present work, we focused on radionuclide molecular probes targeting HER2 and provided a comprehensive overview of different molecular probes used by the current clinical applications (Figure 1). Moreover, we also summarized the developmental trend of some HER2-specific peptides.

2. Radionuclides

Single-photon emission computed tomography (SPECT) and positron emission tomography (PET) are the basic radionuclide imaging modalities [20]. Table 1 lists the commonly used radionuclides for SPECT and PET. The nuclides 99mTc and 111In are widely used in SPECT. However, their poor diagnostic performance limits their clinical application. With the increasing popularity of cyclotrons, a variety of novel positron-emitting radionuclides (such as 18F, 64Cu, 68Ga, and 89Zr) have been widely used in PET imaging. Compared with SPECT, PET has better sensitivity, spatial resolution, and quantization accuracy [21].

3. Radiolabeled HER2-Targeted Monoclonal Antibodies

As the first humanized mAb against HER2, trastuzumab (Herceptin) is approved by the Food and Drug Administration (FDA) for HER2-positive BC, and it has been widely labeled by radionuclide, including 111In, 124I, 64Cu, and 89Zr [22,23,24,25]. Of them, two initial clinical studies using 111In-DTPA-trastuzumab have reported the therapeutic effect and cardiotoxicity of patients with HER2-positive metastatic breast cancer (MBC) treated with trastuzumab [22,26]. Nonetheless, the poor resolution of SPECT may lead to a lower detection rate of HER2-positive tumors. However, trastuzumab labeled with positron nuclides 89Zr and 64Cu significantly improves the image quality. Several clinical studies (Table 2) have shown the value of 89Zr-Df-trastuzumab in detecting HER2-positive lesions and predicted the response to treatment with trastuzumab (Figure 2) as well as other types of treatment and trastuzumab-related toxicity [27,28,29,30]. However, Ulaner et al. have presented findings from a prospective clinical trial of 89Zr-trastuzumab PET/CT in a total of 20 patients with HER2-positive metastases and HER2-negative primary BC [27,28]. Three of these patients develop HER2-positive disease, which is confirmed by immunochemistry (IHC)/fluorescence in situ hybridization (FISH) (NCT02286843). A multi-center study (NCT01565200) consisting of 60 MBC patients reported by Gebhart et al. [31] has investigated the association between the [89Zr]-trastuzumab uptake and outcome after treatment with trastuzumab-emtansine (T-DM1). The study has shown that HER2-targeted imaging in combination with early metabolic response assessment is of great significance for judging the heterogeneity of MBC and for selecting patients who would benefit from T-DM1 treatment. Trastuzumab labeled with 64Cu is an alternative that has also been tested in clinical practice [24,32]. A first-in-human feasibility study of 64Cu-DOTA-trastuzumab has demonstrated successful tumor uptake and visualization of HER2-positive primary BC and metastatic lesions in the brain [24]. The poor visualization of liver metastases due to high background uptake in the liver is a limiting factor for its clinical application. However, if cold trastuzumab is given in advance, high liver uptake can be reduced [33,34].
In addition to trastuzumab, pertuzumab is another FDA-approved mAb targeting HER2. In nuclear medicine, this humanized mAb has been labeled with a variety of radiometals [35,36,37,38,39] and proposed to be used as the imaging agent during monotherapy with trastuzumab [40], since its binding site of HER2 (domain II) is different from trastuzumab (domain IV). The first-in-human trial has demonstrated that 89Zr-pertuzumab is a clinically valuable HER2-targeted imaging agent for MBC patients [37]. In addition, a prospective clinical trial (NCT02286843) has demonstrated that 89Zr-pertuzumab PET/CT helps identify patients with HER2-positive metastases and HER2-negative primary BC [36].

4. Radiolabeled HER2-Targeted Antibody Fragments

The Fab and F(ab′)2 fragments of anti-HER2 antibodies, trastuzumab, and pertuzumab labeled with several different radionuclides have been evaluated for imaging in preclinical models [41,42,43,44]. However, all tested probes have a common feature that HER2-positive tumors can be clearly imaged at 24 h after injection. As reported by Smith-Jones [41], 111In-DOTA-(Fab′)2 provides a tumor-to-blood ratio of 10 already at 24 h, and the tumor uptake of [111In]-DOTA-(Fab′)2 (~20% ID/g) is much lower compared with [111In]-DOTA-trastuzumab (≈50% ID/g). This finding reflects the general feature of antibody fragments, showing lower tumor uptake compared with parental antibodies. Reilly et al. [43] have reported that 64Cu-NOTA-F(ab′)2-pertuzumab can detect changes in the expression of HER2 in response to trastuzumab while delivering a lower total body radiation dose compared with 111In-labeled pertuzumab. Beylergil et al. [44] have reported a clinical study on the diagnostic utility of 68Ga-DOTA-F(ab∲)2-trastuzumab in 15 BC patients (NCT00613847). The results of clinical evaluations are hardly encouraging, since only 4/8 patients with HER2-positive BC are detected.
The long waiting time for imaging of intact radiolabeled antibodies can be solved by using radiolabeled (Fab∲)2 and Fab fragments. However, similar to intact antibodies, the contrast of the main metastatic sites of radiometal-labeled fragments still remains the main problem limiting its clinical application.

5. Radiolabeled HER2-Targeted Nanobodies

Due to the small size and high affinity, nanobodies can penetrate tumor tissues and bind antigens with high specificity, making them a suitable therapeutic and diagnostic tool [45] (Table 3). As an sdAb developed against HER2, 2Rs15d has shown excellent targeting of HER2 in both preclinical settings [46] and clinical settings [47]. A phase I study of 68Ga-NOTA-2Rs15d reported by Keyaerts et al. [47] has shown that its biodistribution is favorable with a fast blood clearance. The primary lesions of BC patients show a wide uptake of 0.7-11.8, while significant uptake is observed in most metastatic lesions, indicating that 68Ga-NOTA-2Rs15d may not be suitable to be used to evaluate the expression of HER2 in primary BC lesions. Currently, a phase II clinical trial (NCT03331601) to evaluate its potential for detecting brain metastases in BC patients and a phase II clinical trial (NCT03924466) to evaluate the correlation between image-based HER2 quantification in the uptake of 68Ga-NOTA-2Rs15d in local or distant metastasis in BC patients are all in progress.
What is more, nanobody 2Rs15d is also labeled with various nuclides, such as 18F, 99mTc, 131I, and 177Lu. Similar to 68Ga-NOTA-2Rs15d, 99mTc-2Rs15d [48] and [18F]RL-II-2Rs15d [49] show high tumor uptake, rapid blood clearance, low accumulation in non-target organs other than the kidneys, and a high tumor to background ratio. Furthermore, the PET imaging of 18F-labeled 2Rs15d with different chelators [49,50] shows significant tumor uptake and substantially lower renal uptake.
2Rs15d is also labeled with a therapeutic radionuclide. In the preclinical study, 131I-SGMIB-2Rs15d [51] and 177Lu-DTPA-2Rs15d [52] have been shown to significantly prolong median survival. A phase I clinical trial (NCT02683083) of 131I-SGMIB-2Rs15d in healthy volunteers and patients with HER2-positive BC shows a high tumor to background ratio, rapid blood clearance, and elimination of unbound nanobodies via the kidney after intravenous administration.
In addition, two single-domain antibodies (MM-302 and NM-02) have been labeled and entered clinical studies. The tumor accumulation of 64Cu-MM-302 ranges from 0.52 to 18.5 %ID/kg after 24-48 h, including deposition in bone and brain lesions [53]. It is worth noting that significant background absorption of 64Cu-MM-302 is observed in the liver and spleen. Currently, the phase I clinical trial of 64Cu-MM-302 (NCT02735798) has been terminated. At present, no results have been reported for another 99mTC-labeled nanobody NM-02 that has entered the clinical early phase I study (NCT04040686).

6. Radiolabeled HER2-Targeted Affibodies

Affibody [54] is a triple helix structure composed of 58 amino acids, which is also called an “artificial antibody”. In contrast to antibodies, the smaller affibody molecules have relatively fast uptake and clearance rates [55]. In addition, the affibody molecules have high affinity and stability, and thus, they are very well suited for molecular imaging (Table 4). The anti-HER2 affibody molecule ZHER2:342 and its derivatives are second-generation HER2-targeted affibodies, which are the most studied affibody imaging probes radiolabeled with different nuclides [56,57,58]. ABY-002 (DOTA ZHER2:342 pep2) is a derivative of ZHER2:342 with a DOTA coupled to its NH2 terminus. The first clinical trial of the radiolabeled HER2-targeted affibody ABY-002 labeled with 111In and 68Ga has demonstrated their potential for visualizing HER2-expressing metastatic lesions in BC patients [59].
As another HER2-targeted affibody, ABY-025 (ZHER2:2891) has been investigated in clinical trials. The 111In-ABY-025 [60] has demonstrated favorable biodistribution, safety, and tumor-targeting potential in patients with HER2-expressing MBC (NCT01216033). It is worth noting that one patient with MBC and HER2-positive primary tumor imaging by 111In-ABY-025 shows a HER2-negative status of the metastases, as confirmed by IHC. Two other clinical trials (NCT02095210 and NCT01858116) have studied the effects of two different doses of 68Ga-ABY-025 (100 μg or 500 μg) on tumor uptake. The PET image shows that the injection of 500 μg 68Ga-ABY-025 leads to a better specificity after 2 to 4 h and allows the differentiation of metastases with HER2 expression levels of 3+ and 2+ [61,62]. 68Ga-ABY-025 is currently being investigated in further clinical research. Furthermore, ABY-025 is also labeled with 18F by using three methods (such as 18F-SiFA, 18F-AlF-NOTA, and 18F-FBA) [63]. Glaser et al. [64] have found that 18F-FBA-ZHER2:2891 (GE226) with enhanced pharmacokinetic characteristics can differentiate tumors with different expression levels of HER2 by imaging. The efficacy of [18F]GE-226 in determining the expression of HER2 in MBC patients is being investigated (NCT03827317). No results have yet been reported for this study yet. Another 99mTc-labeled affibody (HPArk2) is also being investigated in an open-label phase I clinical trial, while no results have been published (NCT04267900).
Hober et al. have reported the development of an affibody ADAPT6 that binds HER2 [65]. The pharmacologic studies have demonstrated that 111In/68Ga-DOTA-(HE)3-ADAPT6 is specifically taken up by HER2+ tumors, with a high tumor-to-normal tissue ratio in mice xenograft tumor models. Furthermore, they have evaluated the effects of the commonly used macrocyclic chelators NOTA, NODAGA, DOTA, and DOTAGA on the biodistribution characteristics of ADAPT6 [66]. It has been concluded that 111In-(HE)3DANS-ADAPT6-GSSC-DOTA is best for SPECT imaging, while 68Ga-(HE)3DANS-ADAPT6-GSSC-NODAGA is best for PET imaging. The phase I clinical study has shown that 99mTc-ADAPT6 is safe, and a protein dose of 500 μg can be used to distinguish tumors with high or low expression of HER2 as early as 2 h after injection [67]. The above-mentioned results indicate that ADAPT6 is a potential molecular imaging probe.

7. Radiolabeled HER2-Targeted Peptides

Peptides have many favorable characteristics suitable for the development of imaging agents. First of all, unlabeled small molecule precursors have the advantages of definite chemical structure, flexible modification space, controllable pharmacokinetic properties, non-immunogenicity, and ease of synthesis commonly involving solid-phase peptide synthesis. Secondly, radionuclide-labeled peptides have higher tissue penetration and faster circulation time in the blood [68,69]. Therefore, imaging of HER2-expressing tumors with radionuclide peptides can lead to quicker imaging time and provide accurate assessment results for treatment based on the expression of HER2. Many peptides have shown promising application value in the detection of HER2 expression in BC (Table 5).

7.1. KCCYSL-Based Peptides

One of the most studied peptides specific for HER2 is the KCCYSL peptide or peptides with KCCYSL incorporated into the sequence. The peptide KCCYSL is first found by Karasseva et al. [70] from a random six-amino acid peptide bacteriophage display library and shows a strong affinity to HER2. Then, it has been evaluated in multiple studies both in vitro and in vivo. KCCYSL is radiolabeled with 111In and 64Cu by Kumar et al. for the imaging of HER2-positive tumors [71,77]. The cell-binding experiments have shown that the radiolabeled peptides 111In-DOTA(GSG)-KCCYSL and 64Cu-(GSG)-KCCYSL can specifically bind to MDA-MB-435 cells (HER2+), while the scrambled peptide 111In-DOTA(GSG)-KYLCSC peptide does not bind to MDA-MB-435 cells. In vivo studies have confirmed that imaging with radiolabeled peptides can specifically identify HER2+ tumors and HER2- in SCID mice at 2 h after injection.
Larimer et al. [72] have developed two peptides, MEGPSKCCYSLALASH (1-D03) and GTKSKCCYSLRRSS (3-G03), which have higher binding affinity compared with the original peptide. Each peptide is radiolabeled with 111In for further studies. Among them, the biodistribution of 111In-DOTA-1-D03 shows that the ratio of tumor to blood after 2 h is about 6. The SPECT imaging shows that radiolabeled peptides can clearly image HER2+ tumors and show specific binding.
The KCCYSL peptides show great potential as a targeting agent for the development of HER2. Radiolabeled versions of this peptide have been shown to have suitable imaging capabilities with SPECT and are hopefully developed as a PET imaging agent.

7.2. LTVSPWY-Based Peptides

Shadidi et al. [73] have used another phage display biopanning procedure that results in the discovery of another HER2-specific peptide (LTVSPWY). Several peptides with LTVSPWY incorporated into the sequence have been radiolabeled by 99mTc and 68Ga for imaging studies of HER2-positive tumors. Sabahnoo et al. [78] have used 99mTc to label two peptides with core LTVSPWY and a cysteine-based ligand (CGGG or CSSS). The 99mTc-CGGG-LTVSPWY and 99mTc-CSSS-LTVSPWY peptides show significantly higher binding to SKOV-3 (HER2+) cells compared with A549 and MCF-7 (HER2-) cells. The competitive binding of trastuzumab has confirmed that these peptides have similar binding sites as trastuzumab. In in vivo studies, mice bearing SKOV-3 xenografts are injected with each peptide to determine the tumor%ID/g after 1 and 4 h. The 99mTc-CGGG-LTVSPWY has a tumor %ID/g of 3.84 ± 2.5 and 2.44 ± 1.1%ID/g at 1 and 4 h, respectively. The 99mTc-CSSS-LTVSPWY peptide has similar values of 4.98 ± 4.8 and 2.26 ± 2.1%ID/g, respectively, at the corresponding time points.
The team of Hosseinimehr has conducted multiple studies on the same core peptide [74,75,79,80]. They have used multiple nuclides to label the peptide with the addition of a new chelator and linker system, SSSLTVPWY. It has been shown that 99mTc-HYNIC-(Ser) 3-LTVPWY can specifically target HER2+ tumors, including SKOV-3 ovarian cancer, at 4 h post-injection, while U-87 MG glioma-based tumors can be visualized after just at 1 h post-injection. In addition, they have also used the EDDA/tricine mixture as a co-ligand to label HYNIC-SSSLTVSPWY with 99mTc. In vivo experiments have shown that 99mTc-HYNIC-(EDDA/tricine)-peptide exhibits noticeable tumor uptake compared with non-target organs after 4 h. However, the ratios of the tumor to blood, or the tumor to muscle for 99mTc-HYNIC-(Ser)3-LTVPWY are 1.32 and 2.65, respectively, and 99mTc-HYNIC(EDDA/tricine)-(Ser)3-LTVSPWY with higher ratios (6.93 and 4.05 respectively) results in better pharmacokinetic modification. The positron nuclide 68Ga is also used to label SSSLTVSPWY. The PET imaging experiments exhibit a capacity of 68Ga-DOTA-(Ser)3-LTVSPWY for imaging the HER2-positive tumors in nude mice with high contrast.

7.3. The Peptide AHNP

The peptide AHNP is obtained by analyzing the CDR3 loop of the heavy chain of trastuzumab that binds to the antigen through a structure-based method by Park et al. [76]. The AHNP has an amino acid sequence of FCGDFYACYMDV with a binding affinity for HER2 of 395 nM. Guan Siao-Syun et al. [81] have used 111 In to label AHNP-PEG with the chelator DTPA and performed potential imaging in patients with HER2-positive gastric cancer. In vivo imaging experiments in xenografts mice induced by NCI-N87 have shown that 111In-DTPA-AHNP-PEG can clearly identify HER2-positive gastric tumors at 1, 4, 24, and 48 h.

7.4. The Peptides H6F and H10F

The H6F(YLFFVFER) [82] and H10F(KLRLEWNR) [83] peptides reported by the group of Wang [84] are discovered by an efficient peptide screening strategy based on in situ single-bead sequencing microarray, and both peptides show high binding and selectivity to HER2. They have used 99mTc to label these two peptides with the chelator HYNIC. 99mTc-HYNIC-H6F and 99mTc-HYNIC-H10F display excellent HER2-binding specificity both in vitro and in vivo. SPECT/CT imaging with 99mTc-HYNIC-H6F/99mTc-HYNIC-H10F in MDA-MB-453, SK-BR3, and MDA-MB-231 xenograft mice model reveals that the HER2+ tumors are clearly visualized, whereas the signals from HER2- tumors are much lower. HER2-positive tumor uptake of 99mTc-HYNIC-H6F and 99mTc-HYNIC-H10F can be blocked by excess unlabeled H6F and H10F but not by excess trastuzumab. In the preliminary clinical study, the 99mTc-HYNIC-H10F has shown potential applications on non-invasive tumor classification, as well as prediction and monitoring of trastuzumab therapeutic efficacy.
Furthermore, Wang et al. [85] have designed a retro-inverso D-peptide of H6 (RDH6) to increase the metabolic stability and added PEGylation to improve its water solubility and in vivo pharmacokinetics. The results show that the D-amino acids 99mTc-PEG4-RDH6 bring better metabolic stability compared with 99mTc-PEG4-H6 with higher tumor uptake. In addition, the introduction of PEG can effectively increase tumor uptake, among which 99mTc-PEG24-RDH6 has a comparable tumor uptake and the lowest liver radioactivity. The SPECT imaging demonstrates that 99mTc-PEG24-RDH6 can specifically distinguish HER2-positive tumors from HER2-negative tumors with better imaging contrast, indicating that the 99mTc-PEG24-RDH6 has the potential for clinical screening of HER2-positive BC.

7.5. Other Peptides

Other potential peptides are also promising for the development of HER2 imaging agents. A9 peptide [86] is synthesized based on the design of trastuzumab (Fab) fragment, and it is synthesized with the chelator DTPA for labeling with 111In. The BT474 cell-binding experiment has revealed the presence of two ligand-binding sites on the receptor target, one with an affinity of Kd = 4.9 nM and the other one with a weaker affinity (Kd = 103 nM). This finding shows that more work is warranted for the A9 peptide in future studies.

8. Conclusions and Outlook

As a broad tumor biomarker, HER2 plays an important role in tumorigenesis. Therefore, accurate assessment of HER2 expression in cancer patients is essential for cancer diagnosis and treatment. Several different anti-HER2 probes, such as monoclonal antibodies, antibody fragments, Affibody molecules, and nanobodies, developed for both SPECT and PET have successfully reached the clinical setting for assessing whole-body HER2 expression patterns (Table 6). Although mAb molecular probes can identify HER2-positive lesions, slow blood clearance, low sensitivity, terrible tumor specificity, and non-specific uptake are still the main problems that limit its clinical transformation. HER2 imaging based on Fab and (Fab’)2 fragments shows good imaging capabilities within 24 h after injection and can solve the issue of long waiting time between the injection of intact radiolabeled antibodies and imaging. However, it must be noted that the tumor uptake of (Fab’)2 fragment is lower than the peak uptake of the parental antibody. Preclinical and clinical studies of small molecular probes, such as nanobodies and affibodies, have shown that they may be the most promising imaging agents. Their high affinity, specific targeting, and rapid clearance rate make it possible for them to evaluate the intertumoral expression heterogeneity and alteration of HER2 status during the disease. However, they still have the problems of high liver and kidney background, high cost, and may not be suitable for the evaluation of HER2-positive primary lesions.
In recent years, more and more small molecule peptides have been developed for HER2 imaging. The HER2-targeted peptides have shown strong potential for imaging HER2-positive tumors. The benefit for the development of these peptides includes the potentially quicker circulation time, deeper tissue penetration, non-immunogenicity, and ease of preparation. However, low tumor uptake levels and tumor-to-organ ratios are still the main problems to be solved in the development of short radiolabeled peptides. One of the main reasons is that the binding affinity of peptides is generally low. Therefore, more research on radiolabeled peptides is required to promote its clinical transformation.
At present, it is hard to say which type of HER2 tracer will be preferred in the clinical setting. We propose that the practical purpose of HER2 imaging will dictate the choice of imaging molecular probes. For instance, low molecular weight probes can be used as an alternative IHC or FISH to diagnose HER2 expression levels in tumors. In contrast, mAbs may be preferred for evaluating the efficacy of a monoclonal antibody-based therapy (e.g., trastuzumab, T-DM1, pertuzumab).
Based on existing preclinical and clinical studies, we are optimistic that HER2-targeted imaging can provide a non-invasive, dynamic visualization and quantification of HER2 expression status in heterogeneous tumors, which will improve the clinical management of HER2-targeted therapy.

Author Contributions

Conceptualization, S.D.; Literature research, S.G., J.L. and Y.Y. (Yu Yu); Writing—original draft preparation, S.G., J.L. and Y.Y. (Yu Yu); Writing—review and editing, S.G., J.L., Y.Y. (Yu Yu), Z.C., Y.Y. (Yi Yang), L.Z., S.S. and S.D. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (grant no. 81601522), Medical Youth Talent Project of Jiangsu Province (grant no. QNRC2016749), Gusu Health Talent Program (grant no. GSWS2020013), Suzhou People’s Livelihood Science and Technology Project (grant no. SYS2019038), Project of State Key Laboratory of Radiation Medicine and Protection, Soochow University (No. GZK1202127) and the open Foundation of Nuclear Medicine Laboratory of Mianyang Central Hospital, (No. 2021HYX023, 2021HYX026 and 2021HYX029).

Institutional Review Board Statement

Not Applicable.

Informed Consent Statement

Not Applicable.

Data Availability Statement

Not Applicable.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are not available from the authors.

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Molecules 26 06482 g001 550
Figure 1. Schematic overview of nuclear imaging for Her2 positive BC. Ligands such as antibody, F(ab’)2 fragments, nanobody, affibody, and peptide can be coupled to a chelator. The chelator enables labeling with different radionuclides that can be applied for imaging purposes.
Figure 1. Schematic overview of nuclear imaging for Her2 positive BC. Ligands such as antibody, F(ab’)2 fragments, nanobody, affibody, and peptide can be coupled to a chelator. The chelator enables labeling with different radionuclides that can be applied for imaging purposes.
Molecules 26 06482 g001
Molecules 26 06482 g002 550
Figure 2. Representative molecular PET images of a patient with HER2-positive BC were visualized by 18F-FDG (A) and HER2 (B) imaging with 89Zr-trastuzumab. Images are reproduced with permission from [30].
Figure 2. Representative molecular PET images of a patient with HER2-positive BC were visualized by 18F-FDG (A) and HER2 (B) imaging with 89Zr-trastuzumab. Images are reproduced with permission from [30].
Molecules 26 06482 g002
Table
Table 1. Nuclides used in PET and SPECT imaging.
Table 1. Nuclides used in PET and SPECT imaging.
NuclideT1/2Production Method
Nuclides for SPECT imaging
99mTc6.01 h99Mo/99mTc Generator
123I13.3 hCyclotron
111In2.8 daysCyclotron
Nuclides for PET imaging
13N9.97 minCyclotron
11C20.4 minCyclotron
68Ga67.6 min68Ge/68Ga Generator
18F109.8 minCyclotron
64Cu12.7 hCyclotron
89Zr78.4 hCyclotron
124I100 hCyclotron
Table
Table 2. The clinical trials of HER2-targeted monoclonal antibodies.
Table 2. The clinical trials of HER2-targeted monoclonal antibodies.
Monoclonal
Antibodies		Nuclide/Chelator or LinkerModalityCondition or DiseasePhase
Trastuzumab89Zr-DfPETHER2-Positive Solid TumorPhase II
NCT04757090
Trastuzumab89ZrPETMetastatic Breast CancerPhase II
NCT01832051
Trastuzumab89Zr-DfPET/MRIBreast CancerEarly Phase I
NCT03321045
Trastuzumab89Zr-DFOPETEsophagogastric CancerNCT02023996
Trastuzumab64Cu-DOTAPETHER2 Positive Breast CarcinomaPhase II
NCT02827877
Trastuzumab64CuPETHER2+ Metastatic Breast CancerPhase I
NCT00605397
Trastuzumab111In-DTPASPECTBreast Cancer
Prostate Cancer
Lung Cancer
Colon Cancer		Early Phase I
NCT01445054
Pertuzumab89Zr-DFOPETHER2-Positive cancerPhase I
NCT03109977
Pertuzumab89Zr-SS
89Zr		PETHER-2 Positive Malignant Carcinoma of Breast
HER2-Positive Metastatic Breast Cancer		Phase I
NCT04692831
Pertuzumab111InSPECTBreast CancerPhase I
NCT01805908
Table
Table 3. The clinical trials of HER2-targeted nanobodies.
Table 3. The clinical trials of HER2-targeted nanobodies.
NanobodiesNuclide/Chelator or LinkerModalityCondition or DiseasePhase
2Rs15d68Ga-NOTAPETBreast Carcinoma
Brain Metastasis of Breast Carcinoma		Phase II
NCT03331601,
NCT03924466
2Rs15d131ISPECTBreast CancerPhase I
NCT02683083
NM-0299mTcSPECTBreast CancerEarly Phase I
NCT04040686
MM-30264CuPETAdvanced HER2+ Cancers with Brain MetsEarly Phase I
NCT02735798
Table
Table 4. The clinical trials of HER2-targeted affibodies.
Table 4. The clinical trials of HER2-targeted affibodies.
AffibodiesNuclide/Chelator or LinkerModalityCondition or DiseasePhase
ABY-02568GaPETHER2-Positive Breast Cancer,
Breast Cancer		Phase I/II
NCT02095210
NCT01858116
ABY-025111InSPECTBreast CancerPhase I/II
NCT01216033
ABH299mTcSPECTBreast CancerEarly Phase I
NCT03546478
HPark299mTcSPECTBreast CancerEarly Phase I
NCT04267900
GE-22618FPETBreast CancerNCT03827317
ADAPT699mTcSPECTBreast CancerNCT03991260
Table
Table 5. Specific HER2-targeted peptides.
Table 5. Specific HER2-targeted peptides.
PeptideLabeling StrategyModalityKd (nM)Reference
KCCYSL111In-DOTA-GSGSPECT295 ± 56[64]
MEGPSKCCYSLALASH111In-DOTASPECT236 ± 83[67]
GTKSKCCYSLRRSS111In-DOTASPECT289 ± 13[67]
CGGGLTVSPWY99mTcSPECT4.3 ± 0.8[69]
CSSSLTVSPWY99mTcSPECT33.9 ± 9.7[69]
SSSLTVPWY99mTc-HYNICSPECT2.6 ± 0.5[70]
SSSLTVPWY99mTc-HYNIC-EDDA/tricineSPECT3.3 ± 1.0[71]
SSSLTVPWY68Ga-DOTAPET2.5 ± 0.6[72]
FCGDFYACYMDV111In-DTPA-peptide-PEGSPECT300[73]
H6F99mTc-HYNICSPECT7.48 ± 3.26[74]
H10F99mTc-HYNICSPECTNA[75]
A9111In-DTPASPECT4.9/103[76]
Table
Table 6. The advantages and shortcomings of different types of molecular probes.
Table 6. The advantages and shortcomings of different types of molecular probes.
Molecular ProbesAdvantageShortcoming
Monoclonal antibodiesIdentify HER2-positive lesionsLow blood clearance, low sensitivity, terrible tumor specificity, and non-specific uptake, higher radiation dose
Antibody fragmentsImaging capabilities within 24 hLow tumor uptake, low lesion detection rate
NanobodiesLow molecular weight, high stability, nanomole level affinity, better tumor penetrationHigher kidney background, unsuitable for evaluation of her2-positive primary breast lesions
AffibodiesDifferent binding sites from monoclonal antibodies, picomole level affinityHigher liver and kidney background
PeptidesPotentially quicker circulation time, deeper tissue penetration, non-immunogenicity, ease of preparationLow tumor uptake and tumor-to-organ ratios
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Ge, S.; Li, J.; Yu, Y.; Chen, Z.; Yang, Y.; Zhu, L.; Sang, S.; Deng, S. Review: Radionuclide Molecular Imaging Targeting HER2 in Breast Cancer with a Focus on Molecular Probes into Clinical Trials and Small Peptides. Molecules 2021, 26, 6482. https://doi.org/10.3390/molecules26216482

AMA Style

Ge S, Li J, Yu Y, Chen Z, Yang Y, Zhu L, Sang S, Deng S. Review: Radionuclide Molecular Imaging Targeting HER2 in Breast Cancer with a Focus on Molecular Probes into Clinical Trials and Small Peptides. Molecules. 2021; 26(21):6482. https://doi.org/10.3390/molecules26216482

Chicago/Turabian Style

Ge, Shushan, Jihui Li, Yu Yu, Zhengguo Chen, Yi Yang, Liqing Zhu, Shibiao Sang, and Shengming Deng. 2021. "Review: Radionuclide Molecular Imaging Targeting HER2 in Breast Cancer with a Focus on Molecular Probes into Clinical Trials and Small Peptides" Molecules 26, no. 21: 6482. https://doi.org/10.3390/molecules26216482

APA Style

Ge, S., Li, J., Yu, Y., Chen, Z., Yang, Y., Zhu, L., Sang, S., & Deng, S. (2021). Review: Radionuclide Molecular Imaging Targeting HER2 in Breast Cancer with a Focus on Molecular Probes into Clinical Trials and Small Peptides. Molecules, 26(21), 6482. https://doi.org/10.3390/molecules26216482

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