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Article

Interaction Study between ESIPT Fluorescent Lipophile-Based Benzazoles and BSA

by
Thais Kroetz
1,
Pablo Andrei Nogara
2,
Fabiano da Silveira Santos
1,3,
Lilian Camargo da Luz
1,
Viktor Saraiva Câmara
1,
João Batista Teixeira da Rocha
2,
Alexandre Gonçalves Dal-Bó
3 and
Fabiano Severo Rodembusch
1,*
1
Grupo de Pesquisa em Fotoquímica Orgânica Aplicada, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Bairro Agronomia, Porto Alegre 91501-970, CEP, Brazil
2
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria-UFSM, Santa Maria 97105-900, RS, Brazil
3
Programa de Pós-Graduação em Ciência e Engenharia de Materiais, Universidade do Extremo Sul Catarinense (UNESC), Av. Universitária 1105, Criciúma 88806-000, CEP, Brazil
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(21), 6728; https://doi.org/10.3390/molecules26216728
Submission received: 9 October 2021 / Revised: 30 October 2021 / Accepted: 4 November 2021 / Published: 6 November 2021
(This article belongs to the Special Issue Photoinduced Proton Transfer Processes Within Heterocyclic Structures)
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Abstract

:
In this study, the interactions of ESIPT fluorescent lipophile-based benzazoles with bovine serum albumin (BSA) were studied and their binding affinity was evaluated. In phosphate-buffered saline (PBS) solution these compounds produce absorption maxima in the UV region and a main fluorescence emission with a large Stokes shift in the blue–green regions due to a proton transfer process in the excited state. The interactions of the benzazoles with BSA were studied using UV-Vis absorption and steady-state fluorescence spectroscopy. The observed spectral quenching of BSA indicates that these compounds could bind to BSA through a strong binding affinity afforded by a static quenching mechanism (Kq~1012 L·mol−1·s−1). The docking simulations indicate that compounds 13 and 16 bind closely to Trp134 in domain I, adopting similar binding poses and interactions. On the other hand, compounds 12, 14, 15, and 17 were bound between domains I and III and did not directly interact with Trp134.

1. Introduction

Albumin is a globular, water-soluble serum protein and is the most abundant protein in the blood. It binds many ligands including fatty acids, trace elements, steroids, thyroid hormones, hemin, calcium, and other molecules [1,2]. This protein is known to play an essential role in the transport and disposition of endogenous and exogenous compounds in blood [3]. Binding studies of therapeutic agents with plasma proteins, particularly serum albumin, provide valuable information on absorption, transportation, distribution, metabolism, and efficacy [4]. Thus, bovine serum albumin (BSA) is frequently chosen as a model for drug–protein interaction studies because of its low cost, availability, and homology with human serum albumin (HSA) [5,6]. BSA exhibits intrinsic fluorescence, mostly due to the indole group of tryptophan (Trp), and the interaction with small molecules can cause fluorescence quenching [7]. In addition, fluorescence titration has been widely applied to study the binding affinity of small molecules to these biomacromolecules, allowing their non-intrusive evaluation under physiological conditions [8]. In this context, 2-(2′-hydroxyphenyl)benzazole-based compounds are privileged compounds owing to their structural and electronic properties [9,10,11]. These fluorophores are able to transfer protons in the excited state due to an intramolecular hydrogen bond between the hydroxyl group (proton donor) and the azolic nitrogen (acceptor group) [12,13]. The photoacidity/photobasicity, which is influenced by bond distance and angles, induces a fast proton transfer to produce a keto phototautomer, which decays and emits fluorescence with a large Stokes shift (approx. 10000 cm−1) [14,15]. These photophysical features allow these compounds to be applied as pH sensors [16,17,18,19], WOLEDs [20,21,22,23,24,25], optical sensors [26,27,28], bioimaging [29,30,31,32,33,34,35], and lipid probes [36,37,38,39,40].
In this study, the binding affinity for BSA of photoactive lipophilic amines based on the benzazolic fluorophore, comprising benzoxazole and benzothiazole compounds, was evaluated. First, their electronic properties were investigated in phosphate buffer solution (PBS) in both the ground and excited states by UV-Vis absorption and steady-state fluorescence emission, respectively. Their application as optical sensors to detect proteins in PBS was successfully explored using BSA as a model. These compounds were chosen due to their high photostability and absent inner-filter effect, which could be interesting for association studies. In addition, the presence of different alkyl chains could also affect their interaction with BSA by suppression studies, provided by hydrophobic interactions with the macromolecule. Finally, molecular modeling studies were performed to better visualize the binding mode with BSA.

2. Results and Discussion

2.1. Synthesis and Photophysical Characterization

The fluorescent secondary amines used in this study are similar to those reported previously [41]. Generally, the reaction of the formyl derivatives 1–2 [14] with an equimolar quantity of amines 3–5 in dry isopropanol/acetic acid (catalyst) under reflux yielded the respective imines 6–11 (Scheme 1). In this step, the solvent was evaporated to dryness to afford 6–11 in a ~90% crude yield, and no further purification was required to proceed with the next step. The desired amines were obtained by reducing the imines with sodium borohydride in a 1:2 (imine:NaBH4) ratio. The final products were obtained after purification via column chromatography in satisfactory yields (~70%) using ethyl acetate as the eluent.
The photophysical investigation was carried out in PBS (pH 7.2) at a concentration of 10−5 M. The characterization data from the electronic ground and excited state are summarized in Table 1. As shown in Figure 1, the benzazoles exhibit absorption maxima in the UV region, as already observed in organic media [41]. The benzothiazoles produced red-shifted absorption maxima compared to their benzoxazole analogs, which can be explained by the better electron delocalization transferred from the sulfur to the oxygen [12]. Surprisingly, both derivatives with short alkyl chains (12 and 15) exhibited absorption maxima at high energies (~330 nm) despite the compounds with longer lipophilic chains, indicating that the alkyl chain somehow plays a role in their electronic properties in the ground state. In addition, for compounds 13–14 and 16–17, broad absorption was observed in the visible region of the spectra. This feature could be a result of scattering and may be an indication of aggregation of these compounds in PBS (pH 7.2), due to the presence of the long alkyl chains in these compounds, which are extremely hydrophobic. Significantly, the similarity in shape and maxima wavelength compared to those of organic solvents, as well as the absence of additional red-shifted bands in PBS, indicates the absence of ionized species in the ground state for derivatives in this media [42,43].
Finally, the UV-Vis absorption spectra were used to calculate the experimental extinction coefficients ε from the oscillator strengths fe using the Strickler–Berg relationship represented by Equation (1) [44]. In this equation, the integral is related to the area of the absorption maxima from a plot of ε (M−1·cm−1) vs. v ¯ (wavenumber, cm−1), correlated to a single electron oscillator.
f e ≈ 4.3 × 10 − 9 ∫   ε d v ¯
The radiative rate constant, ke0, can also be obtained using ε by applying Equation (2), where v ¯ 0 is the absorption maxima (cm−1) [45].
k e 0 ≈ 2.88 × 10 − 9 v ¯ 0 2 ∫   ε d v ¯
The calculated molar absorptivity values (ε, ~104 M−1⋅cm−1), as well as the radiative rate constants (ke0, ~108 s−1) indicate spin and symmetry-allowed electronic transitions, which could be related to 1π-π* transitions. Similar values were obtained for the same compounds in organic media, as discussed in the literature [41]. Moreover, an almost constant radiative lifetime τ0 indicates that these benzazoles seem to populate the same excited state after radiation absorption.
The steady-state fluorescence emission spectra of the studied benzazoles are presented in Figure 2. The curves were obtained by exciting the compounds at the absorption maxima (Table 1). In general, all compounds produced the main emission band located above 450 nm with a large Stokes shift. However, the nature of the emission bands seems to be quite different depending on the hydrophobic portion of the molecule, once again indicating that the alkyl chains influence their photophysics. For the benzoxazole derivatives, compound 12 demonstrated a blue-shifted emission (464 nm, ΔλST 8751 cm−1) when compared to analogs 13 (502 nm, ΔλST 8651 cm−1) and 14 (492 nm, ΔλST 8246 cm−1). Considering the Stokes shift values and the emission maxima location [9], we believe that the emission from compound 12 was produced due to its ionized species. Moreover, from the same parameters, the emission from the additional derivatives 13 and 14 could be related to the ESIPT process (tautomeric emission). Benzothiazole derivatives 15–17 produced similar results. Compound 15, which has a short alkyl chain, emitted fluorescence at 467 nm with a Stokes shift of 8707 cm−1. For 16–17, both the emission maxima (542 nm) and Stokes shifts (~8800 cm−1) were observed at higher wavelengths. Thus, the emission produced by compound 15 is probably related to its ionized species [42]. Compounds 16–17 exhibit proton transfer in the excited state, and the emission is a result of this process. In addition, compounds 16 and 17 produced a weak blue-shifted emission (462 nm), which is also believed to be related to their ionized species. The enol or normal emission was disregarded because of the high Stokes shift values related to these emission bands.

2.2. BSA Binding Study

Interaction studies were carried out to investigate the affinity of benzazoles 12–17 with BSA through fluorescence quenching assays at room temperature (298 K). BSA is composed of a single polypeptide chain of 583 amino acid residues [46] and three domains named I, II, and III, each of which is divided into two subdomains (A and B) [47]. Studies indicate that the main association sites are located in the hydrophobic cavities of the IIA and IIIA subdomains [48]. The BSA structure has two Trp residues, Trp-134 in the first domain, located on the surface of the molecule, and Trp-212 in the IIA subdomain, located in the hydrophobic region of the protein [49,50]. BSA produced an absorption band in the UV-Vis region, with a maximum of around 280 nm, and an intrinsic fluorescence emission with a maximum located between 340–350 nm, depending on the excitation wavelength, owing to the Trp residues [6]. Considering these photophysical properties, the interactions of small molecules with BSA generally lead to the suppression of fluorescence emission, which is a powerful indicator of their interaction mechanisms [3,4,8,51], which are generally classified as dynamic, static, or combined quenching. Static suppression mechanisms are induced by complex formation between BSA and the dye in the ground state. Dynamic suppression is caused by collisions between BSA and the dye in the excited state. In both cases, energy is transferred from the protein to the compound, resulting in the suppression of fluorescence [52]. Thus, to investigate the fluorescence quenching of BSA in the presence of the benzazoles, spectrophotometric titration was performed using different quantities of dye (2–20 μM) and constant BSA concentration (11 μM, 1 mL) in PBS (pH 7.2). This study was performed at room temperature (25 °C), and the suppression percentage was calculated from the emission intensities obtained under excitation at 277 nm.
Figure 3 shows that the UV-Vis absorption bands of the dyes increase in intensity upon addition of the benzoxazoles 12–14 to the solution containing BSA. In addition, the absorption band around 275 nm, related to BSA, also increased due to interference from another interaction that causes fluorescence at a similar wavelength. A similar response was observed for the benzothiazole analogs 15–17 (Figures S1–S3 in Supplementary Materials).
BSA emitted intense fluorescence with a maximum at 334 nm under excitation at 277 nm. The fluorescence emission spectra of BSA in the presence of benzazoles 12–13 shows an initial decrease of approximately 10% upon the first addition of 2 μM quencher (12: 9%, 13: 7%, and 14: 8%) and a significant decrease in the fluorescence intensity at 20 μM dye concentration (12: 42%, 13: 50%, and 14: 41%) with no significant red-shift, suggesting that the interaction site may be close to the Trp residue (Trp-134 or Trp-212). Moreover, these results suggest that there is no perturbation of the microenvironment around the Trp binding site [52]. Similar behavior was observed for the benzothiazole analogs at 2 μM (15: 8%, 16: 6%, and 17: 5%) and at 20 μM (15: 48%, 16: 53%, and 17: 41%; Figures S4–S6). Higher values for BSA quenching were obtained for compounds 13 and 15, which have 12 carbon aliphatic chains, probably due to an optimized balance between hydrophobicity and spatial hindrance.
To identify the main fluorescence quenching mechanism induced by the benzazoles, the Stern–Volmer relationship, described in Equation (3), was applied [53]
F 0 F = 1 + K sv [ Q ] = 1 + k q τ 0 [ Q ]  
where F0 is the fluorescence intensity of pure BSA and F is the fluorescence intensity in the presence of benzazoles (quencher [Q]). KSV is the Stern–Volmer constant, and kq is the bimolecular quenching rate constant, which is related to the suppression efficiency. τ0 is the fluorescence lifetime of BSA in the absence of the suppressor (6.06 ns) [54]. According to this equation, KSV can be obtained from the slope of the linear fit, and kq is equal to KSV/τ0. The obtained curves are presented in Figure 4, and the relevant data are summarized in Table 2. The equations for the respective linear fits are listed in Table S1. High KSV values were obtained (104 M−1), especially for compounds 13 and 16, indicating that the benzazoles exhibit moderate to strong interactions with the BSA binding sites [55]. Additionally, the kq values (~1012 M−1·s−1) exceed the maximum value for the diffusional collision quenching constant according to the Smoluchowski–Stokes–Einstein theory (kdiff ≈ 7.40 × 109 M−1 s−1) [56], which indicates that fluorescence quenching occurred by a static mechanism, in which the formation of a benzazole–BSA conjugate takes place in the ground state.
Thus, given that the reaction was known to proceed via a static mechanism, the binding constant (KA) and the number of binding sites (n) between BSA and the benzazoles were also obtained by applying Equation (4) [57]:
log ( F 0 − F F ) = log   K A + n log   [ Q ]  
where F0 and F represent the fluorescence intensities in the absence and presence of the benzazoles, respectively, and [Q] is the concentration of the quencher (benzazole). The respective curves are shown in Figure 5. Moreover, the standard Gibbs free energy (ΔG0) of the benzazole:BSA conjugates was calculated from the KA values using Equation (5), where R is the gas constant (1.987 cal· K−1·mol−1) and T is the temperature (298 K).
ΔG0 = −RT ln KA
The results from the double logarithmic plot of the relationship between the fluorescence intensities of BSA and the benzazoles are summarized in Table 2, where the KA values are in the order of 104 M−1, suggesting a strong interaction with BSA. The presence of different alkyl chains in the benzazoles is thought to cause variation in the binding constant values, with compounds 13 and 16 exhibiting higher values. In addition, the number of binding sites (n) for all compounds was in the range of 0.88–1.31, indicating the presence of one binding site in the interaction with BSA. The calculated Gibbs free energy did not show any trend, with values in the same order of magnitude indicating spontaneous interaction with BSA.
A BSA association study was also performed with different amounts of protein (0–12 μM in PBS) adding these solutions to the respective benzazole dye solution (2 μM in PBS). This study can be observed in Figure 6, using benzazoles 12 and 15 as models. Figure 6a–c depicts their UV-Vis absorption spectra in the absence and presence of BSA. Two distinct regions can be observed, related to the protein (250–300 nm) and the dyes (300–380 nm), depending on the benzazole. Increasing BSA raised the absorption intensity between 250–300 nm, and the dye region remained almost constant. A similar response was observed for all studied compounds (Figures S7–S10). However—and we would like to highlight these findings—based on the emission spectra (Figure 6b–d), the association was observed only for compound 12, where the fluorescence intensity increased with the BSA amount in solution. All sets of emission spectra and respective plots of fluorescence intensity maxima as a function of BSA concentration are presented in Figures S11–S15.
The first addition of BSA (2 μM) increased the fluorescence intensity by around two times. After the last addition of protein (12 μM) the fluorescence increased by 3.6, and red-shifted the emission maxima 25 nm (Figure 6b). The observed bathochromic shift suggests that the BSA microenvironments are less polar than the PBS due to the hydrophobic groups present in the surface and interiors of the BSA [58]. On the other hand, the parent compound 15 presents an almost constant intensity in the presence of different amounts of BSA (Figure 6d). The additional compounds showed quite different results, sometimes increasing the intensity until reaching a maximum and then decreasing the intensity (compounds 13 and 16) or randomly varying the intensity (compound 14), and sometimes even showing fluorescence suppression (compound 17) (Figures S11–S15). These results indicate that based on the compound’s structure, the interaction with BSA from the point of view of the excited state of the dyes can be a tricky subject. The association (K) of compound 12 with BSA was also obtained by the Benesi–Hildebrand equation for 1:1 complex [59]. Figure 7 presents the plot of 1/(I−I0) vs. 1/[BSA]. The linearity of the plot indicates the formation of a 1:1 complex between compound 12 and BSA. The respective binding constant K calculated from the slope of the straight line was found to be 1.75 × 105 M−1 corroborating with the strong interaction of these compounds with BSA observed by suppression studies.

2.3. Molecular Docking

To better understand the interaction between the studied compounds and BSA, and to identify the amino acid residues involved in their interactions, molecular docking simulations were performed. Initially, blind docking was performed for all protein structures. The results indicate that a possible binding site was in a region close to Trp134. BSA contains two Trp residues (Trp134 and Trp213), both of which are responsible for its fluorescence. Trp134 is located in domain I, while Trp213 is in domain II (Figure 8) [60,61].
Second, we performed semi-flexible docking in the region close to Trp134 (with the lateral chain of residues Arg185, Lys136, Lys131, Trp134, Tyr137, and Tyr160 flexible) to improve the interactions between BSA and the compounds. The docking simulations indicate that compounds 13 and 16 bind closely to Trp134 in domain I (Figure 8) and adopt similar binding poses and interactions (Figure 9b,e). These compounds exhibited π–π stacking between the benzene ring and the indole ring of the Trp134 residue (3.8–4.0 Å), hydrophobic interactions between the methyl group and the imidazole ring from the His18 residue and between the carbon chain of the compounds and the carbon chain from the Lys132 residue. They have hydrogen bonds (H-bonds) between the amine moiety and the carboxylic group of the Glu17 residue and between the hydroxyl moiety and the carbonyl group of the Asn158 residue. There is a π–H bond between the benzoxazole (or benzothiazole) group and the amide moiety of the Asn161 residue and a cation–π interaction between the benzene ring of compound 16 and the ammonium group of the Lys131 residue. These direct interactions between the compounds and the indole ring could influence the Trp fluorescence quenching.
However, compounds 12, 14, 15, and 17 were bound between domains I and III (Figure 8) and did not directly interact with Trp134 (Figure 9). In general, these molecules interacted with the Arg458 residue via cation–π interactions and hydrophobic interactions between the benzoxazole (or benzothiazole) group and the lateral chain of the Ala193 residue and between the methyl moiety and the His145 and Pro146 residues. In addition, we observed that the alkyl chains of compounds 14 and 17 were located in different positions, while the carbon chain of 14 interacted with the Phe133 and Lys136 residues, and the carbon chain of 17 exhibited intramolecular hydrophobic interactions (Figure 9c,f). Previous studies have described this region as a BSA-binding site [63].
These observations are consistent with other studies where π–π interactions and H-bonds are involved in BSA–ligand complex stabilization [64]. In addition, the predicted thermodynamic data (∆Gbind) obtained by molecular docking, differently than observed experimentally, demonstrated that the compounds with short carbon chains (12 and 15) exhibited the lowest ∆Gbind, indicating a more spontaneous interaction with BSA compared with those of other molecules (Table 3).

3. Experimental

3.1. Photophysical Characterization

Spectroscopic grade solvents were used for fluorescence and UV-Vis absorption spectroscopies. UV-Vis absorption spectroscopy of 10−5 M solutions was performed on a Shimadzu UV-2450 spectrophotometer (Shimadzu Corporation, Tokyo, Japan). Steady-state fluorescence spectroscopy was performed using a Shimadzu spectrofluorometer (RF-5301PC). The fluorescence quantum yields (ΦFL) were measured at the optical dilute regime with solutions with an absorbance intensity lower than 0.05 and using quinine sulfate (Riedel-de-Haën, Seelze, Germany) in H2SO4 1N as the quantum yield standard (ΦF = 0.55) [65].

3.2. BSA Interaction Studies

Bovine serum albumin (BSA), lyophilized powder ≥96% (agarose gel electrophoresis) (Sigma-Aldrich, Saint Louis, MO, USA) was used in the protein detection experiments. Phosphate buffered saline (PBS) was prepared from 9.0 g of sodium chloride (NaCl), 1.18 g of monobasic potassium phosphate (KH2PO4), 4.32 g of anhydrous dibasic sodium phosphate (Na2HPO4), and 1.0 L of Milli-Q water, resulting in a saline solution of pH 7.2. To study the interaction modes of BSA with the benzazoles, a stock solution of BSA (2.0 mg·mL−1) with a concentration of 31 µM was initially prepared in PBS at pH 7.2. To analyze the fluorescence quenching of BSA, solutions were prepared with a final volume of 3 mL with a fixed BSA concentration of 11 µM in PBS, pH 7.2. Benzazoles were added to these solutions at different concentrations (0–20 µM) from a stock solution in dimethylformamide. UV-Vis absorption and fluorescence emission spectra were obtained at 25 °C. The emission curves were obtained under excitation at 277 nm, relative to the absorption wavelength of BSA, using Exc/Em slits of 5.0/5.0 nm, respectively. The BSA association study was performed keeping the dye concentration constant (2 μM in PBS). In this study, different amounts of previously prepared BSA solutions (0–10 μM in PBS, pH 7.2) were added. The final solution was kept to rest for 1 h. The fluorescence spectra were obtained at 25 °C and under the excitation of each absorption maxima.

3.3. Molecular Docking

The 3D structure of BSA was obtained from the Protein Data Bank (http://www.rcsb.org/pdb, ID: 4f5s, accessed 1 October 2019) [66]. The Chimera 1.8 software [67] was used to remove chain B, water, and other molecules, and add hydrogen atoms to the BSA protein. The ligands were built in the software Avogadro 1.1.1 [68], following semi-empirical PM6 [69] geometry optimization using the program MOPAC2012 [70]. The ligands and proteins in the pdbqt format were generated by AutoDockTools, where the ligands were considered flexible (with PM6 charges) and the enzyme rigid (with Gasteiger charges) [71]. The AutoDock Vina 1.1.1 program was used for blind docking [72], with a gridbox of 92 × 62 × 86 and the coordinates x = 9.457, y = 23.359, and z = 98.149 (exhaustiveness of 150). Semi-flexible docking was performed for the region surrounding the Trp134 residue, with a gridbox of 30 × 30 × 35 and the coordinates x = 20.324, y = 33.690, and z = 97.801 (exhaustiveness of 150). The lateral chain of the residues Arg185, Lys136, Lys131, Trp134, Tyr137, and Tyr160, was considered flexible during docking to improve the interactions between compounds and proteins. As a model for the binding pose, the compound conformer with the lowest binding energy (ΔG) was selected from the semi-flexible docking experiment. The docking results were analyzed using the Accelrys Discovery Studio 3.5 software [73].

4. Conclusions

In summary, we report here the photophysical characterization of ESIPT fluorescent lipophilic benzazoles and their binding affinity towards BSA by BSA fluorescence quenching and molecular docking. In PBS solution (pH 7.2), these compounds produce absorption maxima in the UV region related to 1π–π* electronic transitions. The derivatives with short alkyl chains exhibited absorption maxima at higher energies, indicating that the alkyl chain influences the electronic properties in the ground state. In addition, compounds with longer alkyl chains demonstrated broad absorption in the visible region, which could be related to the aggregation of these compounds. The benzazoles are fluorescent, with the main emission band located in the blue–green regions with a large Stokes shift. The nature of the emission bands seems to be different depending on the hydrophobic portion of the molecule, once again indicating that the alkyl chains influence their photophysics. The fluorescence quenching of BSA in the presence of the benzazoles demonstrated a significant decrease in the fluorescence intensity without a significant red-shift. The kq values of approximately 1012 M−1·s−1 indicate that the interactions proceed according to a static mechanism. In addition, higher association constant values (KA ~104 M−1) were obtained from the double logarithmic plot relating the fluorescence intensities from BSA and the benzazoles, suggesting a strong interaction with BSA. Association with BSA could be observed only for benzazole 12. The docking simulations indicate that compounds 13 and 16 bind closely to Trp134 in domain I and that the additional compounds were bound between domains I and III, and did not directly interact with Trp134. Finally, the obtained binding parameters indicate that all the studied benzazoles could be efficiently transported and biodistributed by BSA in the bloodstream.

Supplementary Materials

The following are available online in Figures S1–S3: UV-Vis absorption spectra for BSA (11 μM) in PBS (pH 7.2) in the absence and presence of different quantities of benzazoles (0 to 20 μM); Figures S4–S6: Steady-state fluorescence emission spectra in the absence and presence of different quantities of benzazoles (0 to 20 μM) in BSA (11 μM) and PBS (pH 7.2); Figures S7–S14: UV-vis and fluorescence emission spectra from the association studies; Figure S15: Plot of fluorescence intensity maxima as a function of BSA concentration; Table S1: Linear equations and their respective coefficients of determination (R2) from the plot of F0/F vs. [Q] (Stern–Volmer relation) and log [(F0 − F)/F] vs. log [Q] (double log plot).

Author Contributions

Conceptualization, F.S.R.; formal analysis, T.K., P.A.N., F.d.S.S., L.C.d.L. and V.S.C.; funding acquisition, F.S.R.; investigation, T.K., P.A.N., F.d.S.S., L.C.d.L., V.S.C. and J.B.T.d.R.; methodology, T.K.; project administration, F.S.R.; resources, F.S.R., J.B.T.d.R. and A.G.D.-B.; supervision, F.S.R. and J.B.T.d.R.; validation, F.S.R. and J.B.T.d.R.; visualization, F.S.R. and J.B.T.d.R.; writing—original draft, F.S.R., P.A.N. and J.B.T.d.R.; writing—review and editing, F.S.R. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)—Finance Code 001, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant nos. 305954/2019-9 and 154885/2018-5) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Grant no. 17/2551-0000968-1).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Bertucci, C.; Domenici, E. Reversible and covalent binding of drugs to human serum albumin: Methodological approaches and physiological relevance. Curr. Med. Chem. 2002, 9, 1463–1481. [Google Scholar] [CrossRef]
  2. Curry, S.; Mandelkow, H.; Brick, P.; Franks, N. Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites. Nat. Struct. Biol. 1998, 5, 827–835. [Google Scholar] [CrossRef] [PubMed]
  3. Wang, J.; Zhang, Y.Y.; Guo, Y.; Zhang, L.; Xu, R.; Xing, Z.Q.; Wang, S.X.; Zhang, X.D. Interaction of bovine serum albumin with Acridine Orange (CI Basic Orange 14) and its sonodynamic damage under ultrasonic irradiation. Dyes Pigments 2009, 80, 271–278. [Google Scholar] [CrossRef]
  4. Chen, L.B.; Wu, M.H.; Lin, X.C.; Xie, Z.H. Study on the interaction between human serum albumin and a novel bioactive acridine derivative using optical spectroscopy. Luminescence 2011, 26, 172–177. [Google Scholar] [CrossRef] [PubMed]
  5. Gelamo, E.L.; Silva, C.; Imasato, H.; Tabak, M. Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: Spectroscopy and modelling. BBA-Protein Struct. Mol. 2002, 1594, 84–99. [Google Scholar] [CrossRef]
  6. Sulkowska, A. Interaction of drugs with bovine and human serum albumin. J. Mol. Struct. 2002, 614, 227–232. [Google Scholar] [CrossRef]
  7. Abassi, P.; Abassi, F.; Yari, F.; Hashemi, M.; Nafisi, S. Study on the interaction of sulforaphane with human and bovine serum albumins. J. Photochem. Photobiol. B Biol. 2013, 122, 61–67. [Google Scholar] [CrossRef]
  8. Zhang, J.; Xiong, D.X.; Chen, L.N.; Kang, Q.L.; Zeng, B.R. Interaction of pyrrolizine derivatives with bovine serum albumin by fluorescence and UV-Vis spectroscopy. Spectrochim. Acta A 2012, 96, 132–138. [Google Scholar] [CrossRef] [PubMed]
  9. Santos, F.S.; Ramasamy, E.; Ramamurthy, V.; Rodembusch, F.S. Excited state behavior of benzoxazole derivatives in a confined environment afforded by a water soluble octaacid capsule. J. Photochem. Photobiol. A Chem. 2016, 317, 175–185. [Google Scholar] [CrossRef]
  10. Kwon, J.E.; Park, S.Y. Advanced organic optoelectronic materials: Harnessing excited-state intramolecular proton transfer (ESIPT) process. Adv. Mater. 2011, 23, 3615–3642. [Google Scholar] [CrossRef]
  11. Zhao, J.Z.; Ji, S.M.; Chen, Y.H.; Guo, H.M.; Yang, P. Excited state intramolecular proton transfer (ESIPT): From principal photophysics to the development of new chromophores and applications in fluorescent molecular probes and luminescent materials. Phys. Chem. Chem. Phys. 2012, 14, 8803–8817. [Google Scholar] [CrossRef] [PubMed]
  12. Rodembusch, F.S.; Campo, L.F.; Leusin, F.P.; Stefani, V. Excited state intramolecular proton transfer in amino 2-(2’-hydroxyphenyl)benzazole derivatives. Effects of the solvent and the amino group position. J. Lumin. 2007, 126, 728–734. [Google Scholar] [CrossRef]
  13. Lins, G.O.W.; Campo, L.F.; Rodembusch, F.S.; Stefani, V. Novel ESIPT fluorescent benzazolyl-4-quinolones: Synthesis, spectroscopic characterization and photophysical properties. Dyes Pigments 2010, 84, 114–120. [Google Scholar] [CrossRef]
  14. Santos, F.S.; Medeiros, N.G.; Affeldt, R.F.; Duarte, R.D.; Moura, S.; Rodembusch, F.S. Small heterocycles as highly luminescent building blocks in the solid state for organic synthesis. New J. Chem. 2016, 40, 2785–2791. [Google Scholar] [CrossRef]
  15. Berbigier, J.F.; Duarte, L.G.T.A.; Zawacki, M.; de Araújo, B.; Santos, C.; Atvars, T.D.Z.; Gonçalves, P.F.B.; Petzhold, C.L.; Rodembusch, F.S. ATRP initiators based on proton transfer benzazole dyes: Solid state photoactive polymer with very large Stokes shift. ACS Appl. Polym. Mater. 2020, 2, 1406–1416. [Google Scholar] [CrossRef]
  16. Chen, L.N.; Kuo, C.C.; Chiu, Y.C.; Chen, W.C. Ultra metal ions and pH sensing characteristics of thermoresponsive luminescent electrospun nanofibers prepared from poly(HPBO-co-NIPAAm-co-SA). RSC Adv. 2014, 4, 45345–45353. [Google Scholar] [CrossRef]
  17. Santos, F.S.; Duarte, L.G.T.A.; Germino, J.C.; Barboza, C.A.; Atvars, T.D.Z.; Rodembusch, F.S. Photoacidity as tool to rationalize excited state intramolecular proton transfer reactivity in flavonols. Photochem. Photobiol. Sci. 2018, 17, 231–238. [Google Scholar]
  18. Hong, K.I.; Park, S.H.; Lee, S.M.; Shin, I.; Jang, W.D. A pH-sensitive excited state intramolecular proton transfer fluorescent probe for imaging mitochondria and Helicobacter pylori. Sens. Actuators B Chem. 2019, 286, 148–153. [Google Scholar] [CrossRef]
  19. Coelho, F.L.; Santos, F.S.; Duarte, R.C.; Braga, C.A.; Toldo, J.M.; Gonçalves, P.F.B.; Rodembusch, F.S. Benzothiazole merocyanine dyes as middle pH optical sensors. Dyes Pigments 2020, 176, 108193. [Google Scholar] [CrossRef]
  20. Moraes, E.S.; Duarte, L.G.T.A.; Germino, J.C.; Atvars, T.D.Z. Near attack conformation as strategy for ESIPT modulation for white-light generation. J. Phys. Chem. C 2020, 124, 22406–22415. [Google Scholar] [CrossRef]
  21. Tang, K.C.; Chang, M.J.; Lin, T.Y.; Pan, H.A.; Fang, T.C.; Chen, K.Y.; Hung, W.Y.; Hsu, Y.H.; Chou, P.T. Fine tuning the energetics of excited-state intramolecular proton transfer (ESIPT): White light generation in a single ESIPT system. J. Am. Chem. Soc. 2011, 133, 17738–17745. [Google Scholar] [CrossRef] [PubMed]
  22. Duarte, L.G.T.A.; Germino, J.C.; Berbigier, J.F.; Mendes, R.A.; Faleiros, M.M.; Rodembusch, F.S.; Atvars, T.D.Z. The role of a simple and effective salicylidene derivative. Spectral broadening and performance improvement of PFO-based all-solution processed OLEDs. Dyes Pigments 2019, 171, 107671. [Google Scholar] [CrossRef]
  23. Zhang, Z.; Chen, Y.A.; Hung, W.Y.; Tang, W.F.; Hsu, Y.H.; Chen, C.L.; Meng, F.Y.; Chou, P.T. Control of the reversibility of excited-state intramolecular proton transfer (ESIPT) reaction: Host-polarity tuning white organic light emitting diode on a new thiazolo[5,4-d]thiazole ESIPT system. Chem. Mater. 2016, 28, 8815–8824. [Google Scholar] [CrossRef]
  24. Chen, Y.; Fang, Y.; Gu, H.; Qiang, J.; Li, H.; Fan, J.; Cao, J.; Wang, F.; Lu, S.; Chen, X. Color-tunable and ESIPT-inspired solid fluorophores based on benzothiazole derivatives: Aggregation-induced emission, strong solvatochromic effect, and white light emission. ACS Appl. Mater. Interfaces 2020, 12, 55094–55106. [Google Scholar] [CrossRef]
  25. Duarte, L.G.T.A.; Germino, J.C.; Berbigier, J.F.; Barboza, C.A.; Faleiros, M.M.; Simoni, D.A.; Galante, M.T.; de Holanda, M.S.; Rodembusch, F.S.; Atvars, T.D.Z. White-light generation on all-solution-processed OLEDs using a benzothiazole-salophen derivative reactive to the ESIPT process. Phys. Chem. Chem. Phys. 2019, 21, 1172–1182. [Google Scholar] [CrossRef]
  26. Chen, L.; Fu, P.Y.; Wang, H.P.; Pan, M. Excited-state intramolecular proton transfer (ESIPT) for optical sensing in solid state. Adv. Opt. Mater. 2021. [Google Scholar] [CrossRef]
  27. Sedgwick, A.C.; Wu, L.; Han, H.H.; Bull, S.D.; He, X.P.; James, T.D.; Sessler, J.L.; Tang, B.Z.; Tian, H.; Yoon, J. Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents. Chem. Soc. Rev. 2018, 47, 8842–8880. [Google Scholar] [CrossRef] [Green Version]
  28. Li, Y.; Dahal, D.; Abeywickrama, C.S.; Pang, Y. Progress in tuning emission of the excited-state intramolecular proton transfer (ESIPT)-based fluorescent probes. ACS Omega 2021, 6, 6547–6553. [Google Scholar] [CrossRef] [PubMed]
  29. Zheng, Q.; Ding, F.; Hu, X.; Feng, J.; Shen, J.; He, X. ESIPT-based fluorescent probe for bioimaging and identification of group IIIA ions in live cells and zebrafish. Bioorg. Chem. 2021, 109, 104746. [Google Scholar] [CrossRef]
  30. Singla, N.; Ahmad, M.; Dhiman, S.; Kumar, G.; Singh, S.; Verma, S.; Kaur, S.; Rashid, M.; Kaur, S.; Luxami, V.; et al. An ESIPT based versatile fluorescent probe for bioimaging strong acidic conditions in live-cells and E. coli. New J. Chem. 2021, 45, 19145–19153. [Google Scholar] [CrossRef]
  31. da Silva, C.B.; Gil, E.S.; Santos, F.S.; Morás, A.M.; Steffens, L.; Gonçalves, P.F.B.; Moura, D.J.; Lüdtke, D.S.; Rodembusch, F.S. Proton-transfer based azides with fluorescence off-on response for detection of hydrogen sulfide. An experimental, theoretical and bioimaging study. J. Org. Chem. 2018, 83, 15210–15224. [Google Scholar] [CrossRef] [PubMed]
  32. Tu, Z.; Liu, M.; Qian, Y.; Yang, G.; Cai, M.; Wang, L.; Huang, W. Easily fixed simple small ESIPT molecule with aggregation induced emission for fast and photostable “turn-on” bioimaging. RSC Adv. 2015, 5, 7789–7793. [Google Scholar] [CrossRef]
  33. Zhou, X.; Jiang, Y.; Zhao, X.; Guo, D. ESIPT-based photoactivatable fluorescent probe for ratiometric spatiotemporal bioimaging. Sensors 2016, 16, 1684. [Google Scholar] [CrossRef] [Green Version]
  34. De Souza, V.P.; Santos, F.S.; Rodembusch, F.S.; Braga, C.B.; Ornelas, C.; Pilli, R.A.; Russowsky, D. Hybrid dihydropyrimidinones as dual-functional bioactive molecules: Fluorescent probes and cytotoxic agents to cancer cells. New J. Chem. 2020, 44, 12440–12451. [Google Scholar] [CrossRef]
  35. Lv, H.M.; Yuan, D.H.; Liu, W.; Chen, Y.; Au, C.T.; Yin, S.F. A highly selective ESIPT-based fluorescent probe for cysteine sensing and its bioimaging application in living cells. Sens. Actuators B Chem. 2016, 233, 173–179. [Google Scholar] [CrossRef]
  36. Sulaiman, S.A.J.; Al-Rasbi, G.S.; Abou-Zied, O.K. Photophysical properties of hydroxyphenyl benzazoles and their applications as fluorescent probes to study local environment in DNA, protein, and lipid. Luminescence 2016, 31, 614–625. [Google Scholar] [CrossRef] [PubMed]
  37. Abou-Zied, O.K.; Zahid, N.I.; Khyasudeen, M.F.; Giera, D.S.; Thimm, J.C.; Hashim, R. Detecting local heterogeneity and ionization ability in the head group region of different lipidic phases using modified fluorescent probes. Sci. Rep. 2015, 5, 8699. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Dick, P.F.; Coelho, F.L.; Rodembusch, F.S.; Campo, L.F. Amphiphilic ESIPT benzoxazole derivatives as prospective fluorescent membrane probes. Tetrahedron Lett. 2014, 55, 3024–3029. [Google Scholar] [CrossRef]
  39. Jiang, N.; Yang, C.; Dong, X.; Sun, X.; Zhang, D.; Liu, C. An ESIPT fluorescent probe sensitive to protein α-helix structures. Org. Biomol. Chem. 2014, 12, 5250–5259. [Google Scholar] [CrossRef] [PubMed]
  40. Coelho, F.L.; Rodembusch, F.S.; Campo, L.F. Synthesis, characterization and photophysics of new photoactive ESIPT lipophilic dyes. Partition experiments with different composed liposomes. Dyes Pigments 2014, 110, 134–142. [Google Scholar] [CrossRef]
  41. Kroetz, T.; dos Santos, M.C.; Beal, R.; Zanotto, G.M.; Santos, F.S.; Giacomelli, F.C.; Gonçalves, P.F.B.; de Lima, V.R.; Dal-Bó, A.G.; Rodembusch, F.S. Proton transfer fluorescent secondary amines. Synthesis, photophysics, theoretical calculation and preparation of photoactive phosphatidylcholine-based liposomes. Photochem. Photobiol. Sci. 2019, 18, 1171–1184. [Google Scholar] [CrossRef]
  42. Duarte, L.G.T.A.; Rodembusch, F.S.; Atvars, T.D.Z.; Weiss, R.G. Experimental and theoretical investigation of excited-state intramolecular proton transfer processes of benzothiazole derivatives in amino-polydimethylsiloxanes before and after crosslinking by CO2. J. Phys. Chem. A 2020, 124, 288–299. [Google Scholar] [CrossRef]
  43. Santos, F.S.; Ramasamy, E.; Ramamurthy, V.; Rodembusch, F.S. Photoinduced electron transfer across an organic molecular wall: Octaacid encapsulated ESIPT dyes as electron donors. Photochem. Photobiol. Sci. 2017, 16, 840–844. [Google Scholar] [CrossRef] [PubMed]
  44. Strickler, S.J.; Berg, R.A. Relationship between absorption intensity and fluorescence lifetime of molecules. J. Phys. Chem. 1962, 37, 814–822. [Google Scholar] [CrossRef] [Green Version]
  45. Turro, N.J.; Scaiano, J.C.; Ramamurthy, V. Principles of Molecular Photochemistry: An Introduction, 1st ed.; University Science Books: Sausalito, CA, USA, 2008. [Google Scholar]
  46. Hirayama, K.; Akashi, S.; Furuya, M.; Fukuhara, K. Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS. Biochem. Biophys. Res. Commun. 1990, 173, 639–646. [Google Scholar] [CrossRef]
  47. Kaur, A.; Banipal, P.; Banipal, T. Local anesthetic-bovine serum albumin interactional behaviour: Characterization by volumetric, calorimetric, and spectroscopic methods. J. Mol. Liq. 2017, 243, 91–101. [Google Scholar] [CrossRef]
  48. Thakur, A.; Patwa, J.; Pant, S.; Sharma, A.; Flora, S.J.S. Interaction study of monoisoamyl dimercaptosuccinic acid with bovine serum albumin using biophysical and molecular docking approaches. Sci. Rep. 2021, 11, 4068. [Google Scholar] [CrossRef]
  49. Majorek, K.A.; Porebski, P.J.; Dayal, A.; Zimmerman, M.D.; Jablonska, K.; Stewart, A.J.; Chruszcz, M.; Minor, W. Structural and immunologic characterization of bovine, horse, and rabbit serum albumins. Mol. Immunol. 2012, 52, 174–182. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  50. Roufegarinejad, L.; Jahanban-Esfahlan, A.; Sajed-Amin, S.; Panahi-Azar, V.; Tabibiazar, M. Molecular interactions of thymol with bovine serum albumin: Spectroscopic and molecular docking studies. J. Mol. Recognit. 2018, 31, e2704. [Google Scholar] [CrossRef]
  51. Borba, L.C.; Griebeler, C.H.; Bach, M.F.; Barboza, C.A.; Nogara, P.A.; da Rocha, J.B.T.; Amaral, S.S.; Rodembusch, F.S.; Schneider, P.H. Non-traditional intrinsic luminescence of amphiphilic-based ionic liquids from oxazolidines: Interaction studies in phosphatidylcholine composed liposomes and BSA optical sensing in solution. J. Mol. Liq. 2020, 313, 113525. [Google Scholar] [CrossRef]
  52. Lakowicz, J.R. Principles of Fluorescence Spectroscopy, 3rd ed.; Springer: New York, NY, USA, 2006. [Google Scholar]
  53. Gehlen, M.H. The centenary of the Stern-Volmer equation of fluorescence quenching: From the single line plot to the SV quenching map. J. Photochem. Photobiol. C Photochem. Rev. 2020, 42, 100338. [Google Scholar] [CrossRef]
  54. Chaves, O.A.; Cesarin-Sobrinho, D.; Sant’Anna, C.M.R.; de Carvalho, M.G.; Suzart, L.R.; Catunda, F.E.A.; Netto-Ferreira, J.C.; Ferreira, A.B.B. Probing the interaction between 7-O-beta-D-glucopyranosyl-6-(3-methylbut-2-enyl)-5,4’-dihydroxyflavonol with bovine serum albumin (BSA). J. Photochem. Photobiol. A Chem. 2017, 336, 32–41. [Google Scholar] [CrossRef]
  55. Naveenraj, S.; Anandan, S. Binding of serum albumins with bioactive substances—Nanoparticles to drugs. J. Photochem. Photobiol. C 2013, 14, 53–71. [Google Scholar] [CrossRef]
  56. Montalti, M.; Credi, A.; Prodi, L.; Gandolfi, M.T. Handbook of Photochemistry, 3rd ed.; CRC Press: Boca Raton, FL, USA, 2006. [Google Scholar]
  57. Wang, C.; Wu, Q.H.; Wang, Z.; Zhao, J. Study of the interaction of Carbamazepine with bovine serum albumin by fluorescence quenching method. Anal. Sci. 2006, 22, 435–438. [Google Scholar] [CrossRef] [Green Version]
  58. Pisoni, D.S.; Todeschini, L.; Borges, A.C.A.; Petzhold, C.L.; Rodembusch, F.S.; Campo, L.F. Symmetrical and asymmetrical cyanine dyes. Synthesis, spectral properties and BSA association study. J. Org. Chem. 2014, 79, 5511–5522. [Google Scholar] [CrossRef]
  59. Rajamohan, R.; Nayaki, S.K.; Swaminathan, M. Inclusion complexation and photoprototropic behaviour of 3-amino-5-nitrobenzisothiazole with β-cyclodextrin. Spectrochim. Acta A 2008, 69, 371–377. [Google Scholar] [CrossRef] [PubMed]
  60. Kragh-Hansen, U. Molecular aspects of ligand binding to serum albumin. Pharmacol. Rev. 1981, 33, 17–53. [Google Scholar]
  61. Moriyama, Y.; Ohta, D.; Hachiya, K.; Mitsui, Y.; Takeda, K. Fluorescence behavior of tryptophan residues of bovine and human serum albumins in ionic surfactant solutions: A comparative study of the two and one tryptophan(s) of bovine and human albumins. J. Protein Chem. 1996, 15, 265–272. [Google Scholar] [CrossRef] [PubMed]
  62. Panigrahi, G.K.; Suthar, M.K.; Verma, N.; Asthana, S.; Tripathi, A.; Gupta, S.K.; Saxena, J.K.; Raisuddin, S.; Das, M. Investigation of the interaction of anthraquinones of Cassia occidentalis seeds with bovine serum albumin by molecular docking and spectroscopic analysis: Correlation to their in vitro cytotoxic potential. Food Res. Int. 2015, 77, 368–377. [Google Scholar] [CrossRef]
  63. Karami, K.; Rafiee, M.; Lighvan, Z.M.; Zakariazadeh, M.; Faal, A.Y.; Esmaeili, S.A.; Momtazi-Borojeni, A.A. Synthesis, spectroscopic characterization and in vitro cytotoxicities of new organometallic palladium complexes with biologically active β-diketones; Biological evaluation probing of the interaction mechanism with DNA/Protein and molecular docking. J. Mol. Struct. 2018, 1154, 480–495. [Google Scholar] [CrossRef]
  64. Wani, T.A.; Bakheit, A.H.; Abounassif, M.A.; Zargar, S. Study of Interactions of an anticancer drug Neratinib with bovine serum albumin: Spectroscopic and molecular docking approach. Front. Chem. 2018, 6, 47. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Chekalyuk, A.; Fadeev, V.; Georgiev, G.; Kalkanjjev, T.; Nickolov, Z. Determination of fluorescence quantum yields using a spontaneous Raman scattering line of the solvent as internal standard. Spectrosc. Lett. 1982, 15, 355–365. [Google Scholar] [CrossRef]
  66. Bujacz, A. Structures of bovine, equine and leporine serum albumin. Acta Crystallogr. Sect. D 2012, 68, 1278–1289. [Google Scholar] [CrossRef] [PubMed]
  67. Pettersen, E.F.; Goddard, T.D.; Huang, C.C.; Couch, G.S.; Greenblatt, D.M.; Meng, E.C.; Ferrin, T.E. UCSF Chimera—A visualization system for exploratory research and analysis. J. Comput. Chem. 2004, 25, 1605–1162. [Google Scholar] [CrossRef] [Green Version]
  68. Hanwell, M.D.; Curtis, D.E.; Lonie, D.C.; Vandermeersch, T.; Zurek, E.; Hutchison, G.R. Avogadro: An advanced semantic chemical editor, visualization, and analysis platform. J. Cheminform. 2012, 4, 17. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  69. Stewart, J.J.P. Optimization of parameters for semiempirical methods V: Modification of NDDO approximations and application to 70 elements. J. Mol. Mod. 2007, 13, 1173–1213. [Google Scholar] [CrossRef] [Green Version]
  70. Stewart, J.J.P. MOPAC2012; Stewart Computational Chemistry: Colorado Springs, CO, USA, 2012; Available online: http://OpenMOPAC.net (accessed on 15 November 2019).
  71. Morris, G.M.; Huey, R.; Lindstrom, W.; Sanner, M.F.; Belew, R.K.; Goodsell, D.S.; Olson, A.J. AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J. Comput. Chem. 2009, 30, 2785–2791. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  72. Trott, O.; Olson, A.J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J. Comput. Chem. 2010, 31, 455–461. [Google Scholar] [CrossRef] [Green Version]
  73. Dassault Systèmes BIOVIA. Discovery Studio Modeling Environment, Release 2017; Dassault Systèmes: San Diego, CA, USA, 2017. [Google Scholar]
Molecules 26 06728 sch001 550
Scheme 1. Synthesis of the ESIPT fluorescent lipophile-based benzazoles 12–17, under the following conditions: (i) iPrOH, CH3COOH, reflux, 5–24 h, and (ii) iPrOH, NaBH4.
Scheme 1. Synthesis of the ESIPT fluorescent lipophile-based benzazoles 12–17, under the following conditions: (i) iPrOH, CH3COOH, reflux, 5–24 h, and (ii) iPrOH, NaBH4.
Molecules 26 06728 sch001
Molecules 26 06728 g001 550
Figure 1. UV-Vis absorption spectra in PBS (pH 7.2) (approx. 10−5 M) of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Figure 1. UV-Vis absorption spectra in PBS (pH 7.2) (approx. 10−5 M) of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Molecules 26 06728 g001
Molecules 26 06728 g002 550
Figure 2. Steady-state fluorescence emission spectra in PBS (pH 7.2) for (approx. 10−5 M) of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Figure 2. Steady-state fluorescence emission spectra in PBS (pH 7.2) for (approx. 10−5 M) of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Molecules 26 06728 g002
Molecules 26 06728 g003 550
Figure 3. UV-Vis absorption (left) and steady-state fluorescence emission spectra (right) for BSA (11 μM) in PBS (pH 7.2) in the absence and presence of different quantities (0 to 20 μM) of compounds (a) 12, (b) 13, and (c) 14. The asterisk indicates the excitation wavelength signal. (For emission spectra λex = 277 nm, slits Ex/Em = 5.0 nm/5.0 nm).
Figure 3. UV-Vis absorption (left) and steady-state fluorescence emission spectra (right) for BSA (11 μM) in PBS (pH 7.2) in the absence and presence of different quantities (0 to 20 μM) of compounds (a) 12, (b) 13, and (c) 14. The asterisk indicates the excitation wavelength signal. (For emission spectra λex = 277 nm, slits Ex/Em = 5.0 nm/5.0 nm).
Molecules 26 06728 g003
Molecules 26 06728 g004 550
Figure 4. The linear plot from the Stern–Volmer relationship of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Figure 4. The linear plot from the Stern–Volmer relationship of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Molecules 26 06728 g004
Molecules 26 06728 g005 550
Figure 5. Double logarithm plots for the KA and n calculations of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Figure 5. Double logarithm plots for the KA and n calculations of ESIPT fluorescent lipophilic (a) benzoxazoles 12–14 and (b) benzothiazoles 15–17.
Molecules 26 06728 g005
Molecules 26 06728 g006 550
Figure 6. UV-Vis and fluorescence emission spectra of the benzazoles (2 μM) 12 (a,b) and 15 (c,d) in different BSA concentrations (0–12 μM). The insert presents the respective plot of fluorescence intensity maxima as a function of BSA concentration.
Figure 6. UV-Vis and fluorescence emission spectra of the benzazoles (2 μM) 12 (a,b) and 15 (c,d) in different BSA concentrations (0–12 μM). The insert presents the respective plot of fluorescence intensity maxima as a function of BSA concentration.
Molecules 26 06728 g006
Molecules 26 06728 g007 550
Figure 7. Benesi–Hildebrand fluorescence plot for 1:1 complexation of compound 12 with BSA (R2 = 0.994).
Figure 7. Benesi–Hildebrand fluorescence plot for 1:1 complexation of compound 12 with BSA (R2 = 0.994).
Molecules 26 06728 g007
Molecules 26 06728 g008 550
Figure 8. Binding sites of the ESIPT lipophilic benzazoles 12–17 predicted by semi-flexible docking. Only compounds 13 and 16 interact directly with the Trp134 residue. The benzazoles are represented by ball and stick models. The domain regions comprise the following: domain I (residues 1–195), domain II (residues 196–383), and domain III (residues 384–583) [62].
Figure 8. Binding sites of the ESIPT lipophilic benzazoles 12–17 predicted by semi-flexible docking. Only compounds 13 and 16 interact directly with the Trp134 residue. The benzazoles are represented by ball and stick models. The domain regions comprise the following: domain I (residues 1–195), domain II (residues 196–383), and domain III (residues 384–583) [62].
Molecules 26 06728 g008
Molecules 26 06728 g009 550
Figure 9. Interactions between the compounds (a) 12; (b) 13; (c) 14; (d) 15; (e) 16, and (f) 17 and the main residues of the BSA protein. Distances are presented in Å.
Figure 9. Interactions between the compounds (a) 12; (b) 13; (c) 14; (d) 15; (e) 16, and (f) 17 and the main residues of the BSA protein. Distances are presented in Å.
Molecules 26 06728 g009
Table
Table 1. Photophysical data of ESIPT fluorescent lipophilic benzoxazoles 12–17 from UV-Vis absorption and fluorescence emission spectroscopies, where ε is the molar extinction coefficient, λabs and λem are the absorption and emission maxima, respectively, fe is the calculated oscillator strength, ke0 is the calculated radiative rate constant, τ0 is the calculated pure radiative lifetime, ΔλST is the Stokes shift, and ΦFL is the fluorescence quantum yield.
Table 1. Photophysical data of ESIPT fluorescent lipophilic benzoxazoles 12–17 from UV-Vis absorption and fluorescence emission spectroscopies, where ε is the molar extinction coefficient, λabs and λem are the absorption and emission maxima, respectively, fe is the calculated oscillator strength, ke0 is the calculated radiative rate constant, τ0 is the calculated pure radiative lifetime, ΔλST is the Stokes shift, and ΦFL is the fluorescence quantum yield.
Benzazoleλabs
(nm)		ε
(104 M−1·cm−1)		feke0
(108 s−1)		τ0a
(ns)		λem
(nm)		ΔλST
(cm−1)		ΦFL
(%)
123301.530.8958.221.2246487510.069
133501.780.9067.391.3550286510.094
143502.020.9437.701.3049282460.144
153321.400.8607.801.2846787070.087
163671.390.7735.741.7454287980.099
173651.360.7895.921.6954289470.148
a τ0 = 1/ke0.
Table
Table 2. BSA-binding parameters for benzazoles 12–17, where Q is the quenching percentage, KSV is the Stern–Volmer quenching constant, kq is the bimolecular quenching rate constant, Ka is the binding constant, n is the number of binding sites, and ΔG0 is the Gibbs free energy for the BSA–benzazole interaction.
Table 2. BSA-binding parameters for benzazoles 12–17, where Q is the quenching percentage, KSV is the Stern–Volmer quenching constant, kq is the bimolecular quenching rate constant, Ka is the binding constant, n is the number of binding sites, and ΔG0 is the Gibbs free energy for the BSA–benzazole interaction.
BenzazoleQ[a]
(%)		KSV
(×104 M−1)		kq
(×1012 M−1·s−1)		KA
(×104 M−1)		nΔG0[b]
(kcal·mol−1)
12423.145.180.610.92−5.16
13505.128.450.741.16−5.28
14413.185.250.590.88−5.14
15484.667.690.731.15−5.27
16535.629.270.791.31−5.31
17413.465.710.691.09−5.23
[a]Q(%) = (Iinitial − Ifinal)/Iinitial × 100; [b]ΔG0 = −RT ln KA.
Table
Table 3. Predicted ∆Gbind from the semi-flexible docking of molecules 19–24 with BSA.
Table 3. Predicted ∆Gbind from the semi-flexible docking of molecules 19–24 with BSA.
Compound121314151617
∆G bind (kcal·mol−1)−8.0−6.8−6.5−8.0−6.9−6.4
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Kroetz, T.; Nogara, P.A.; da Silveira Santos, F.; da Luz, L.C.; Câmara, V.S.; da Rocha, J.B.T.; Gonçalves Dal-Bó, A.; Rodembusch, F.S. Interaction Study between ESIPT Fluorescent Lipophile-Based Benzazoles and BSA. Molecules 2021, 26, 6728. https://doi.org/10.3390/molecules26216728

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Kroetz T, Nogara PA, da Silveira Santos F, da Luz LC, Câmara VS, da Rocha JBT, Gonçalves Dal-Bó A, Rodembusch FS. Interaction Study between ESIPT Fluorescent Lipophile-Based Benzazoles and BSA. Molecules. 2021; 26(21):6728. https://doi.org/10.3390/molecules26216728

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Kroetz, Thais, Pablo Andrei Nogara, Fabiano da Silveira Santos, Lilian Camargo da Luz, Viktor Saraiva Câmara, João Batista Teixeira da Rocha, Alexandre Gonçalves Dal-Bó, and Fabiano Severo Rodembusch. 2021. "Interaction Study between ESIPT Fluorescent Lipophile-Based Benzazoles and BSA" Molecules 26, no. 21: 6728. https://doi.org/10.3390/molecules26216728

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