Anticancer Activity of Continentalic Acid in B-Cell Lymphoma
Abstract
:1. Introduction
2. Results
2.1. Aralia continentalis Extract Induces Apoptosis of Human Lymphoma Cell Line
2.2. Three Diterpenes from A. continentalis Extracts Induce Apoptosis in Human Lymphoma Cell Lines
2.3. Three Dipertenes from A. continentalis Extracts Have Minimal Toxicity towards Normal Cells
2.4. Continentalic Acid Induces Apoptosis Depending on Caspase and Mitochondria by Regulating Bcel-2 Family Members
2.5. Synergy between Roflumilast and Continentalic Acid
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Antibodies
4.2. Preparation of Aralia continentalis Extracts and Isolation of Single Compounds
4.3. Cell Viability and Apoptosis Assays
4.4. Analysis of Caspase 3/7 Activity and Mitochondrial Membrane Potential
4.5. Western Blot Analysis
4.6. Animal Studies
4.7. Hematoxylin and Eosin (H&E) Staining
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Jeon, B.-E.; Kwon, C.-S.; Lee, J.-E.; Moon, K.; Cha, J.; Park, I.; Koh, S.; Yoon, M.; Kim, S.-W.; Kim, J.N. Anticancer Activity of Continentalic Acid in B-Cell Lymphoma. Molecules 2021, 26, 6845. https://doi.org/10.3390/molecules26226845
Jeon B-E, Kwon C-S, Lee J-E, Moon K, Cha J, Park I, Koh S, Yoon M, Kim S-W, Kim JN. Anticancer Activity of Continentalic Acid in B-Cell Lymphoma. Molecules. 2021; 26(22):6845. https://doi.org/10.3390/molecules26226845
Chicago/Turabian StyleJeon, Byeol-Eun, Chan-Seong Kwon, Ji-Eun Lee, Keumok Moon, Jaeho Cha, Inmyoung Park, Sara Koh, Myunghee Yoon, Sang-Woo Kim, and Jeong Nam Kim. 2021. "Anticancer Activity of Continentalic Acid in B-Cell Lymphoma" Molecules 26, no. 22: 6845. https://doi.org/10.3390/molecules26226845