ThermoSlope: A Software for Determining Thermodynamic Parameters from Single Steady-State Experiments
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Software Implementation
3.2. Recombinant Protein Production
3.3. Experimental Setup
3.4. Differential Scanning Calorimetry
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Temperature (K) | kcat (s−1) | |||
---|---|---|---|---|
Stepwise | Gradient | Stepwise | Gradient | |
277 | 400 ± 400 | 50 ± 10 | ||
281 | 250 ± 50 | 132 ± 7 | ||
282 | 160 ± 60 | 110 ± 10 | ||
283 | 1300 ± 800 | 190 ± 50 | ||
284 | 200 ± 40 | 149 ± 9 | ||
285 | 250 ± 50 | 200 ± 10 | ||
287 | 180 ± 70 | 200 ± 30 | ||
288 | 500 ± 200 | 200 ± 20 | ||
289 | 260 ± 40 | 250 ± 10 | ||
290 | 180 ± 20 | 240 ± 10 | ||
292 | 270 ± 60 | 300 ± 20 | ||
293 | 300 ± 100 | 240 ± 40 | 330 ± 40 | 320 ± 20 |
295 | 290 ± 60 | 370 ± 20 | ||
296 | 210 ± 30 | 360 ± 20 | ||
298 | 500 ± 100 | 120 ± 20 | 310 ± 20 | 312 ± 10 |
BLA | PPA | AHA | pLipA | |
---|---|---|---|---|
21.8 ± 0.2 | 18.556 ± 0.005 | 16.782 ± 0.005 | 13.83 ± 0.06 | |
18 ± 1 | 13.5 ± 0.3 | 16.3 ± 0.4 | 15 ± 2 | |
−4 ± 1 | −5.0 ± 0.3 | −0.5 ± 0.4 | 1 ± 2 |
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Lund, B.A.; Brandsdal, B.O. ThermoSlope: A Software for Determining Thermodynamic Parameters from Single Steady-State Experiments. Molecules 2021, 26, 7155. https://doi.org/10.3390/molecules26237155
Lund BA, Brandsdal BO. ThermoSlope: A Software for Determining Thermodynamic Parameters from Single Steady-State Experiments. Molecules. 2021; 26(23):7155. https://doi.org/10.3390/molecules26237155
Chicago/Turabian StyleLund, Bjarte Aarmo, and Bjørn Olav Brandsdal. 2021. "ThermoSlope: A Software for Determining Thermodynamic Parameters from Single Steady-State Experiments" Molecules 26, no. 23: 7155. https://doi.org/10.3390/molecules26237155