Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction
Abstract
:1. Introduction
2. Results
2.1. Bag-1 Interacts with Beclin 1
2.2. Bag-1S Isoform, but Not Bag-1L Isoform Intracellularly Co-Localizes with Beclin 1
2.3. Bag-1/Beclin 1 Interaction Occurs before Induction of Autophagy
2.4. Bag-1 Does Not Directly Interact with Beclin 1 In Vitro
2.5. Bag-1 Deficiency Impairs Cell Survival and Proliferation
2.6. Bag-1 Participates in Autophagic Regulation in MCF-7 Cells
3. Discussion
4. Materials and Methods
4.1. DNA Constructs and siRNAs
4.2. Antibodies
4.3. Cell Lines, Cell Culture and Transient Transfection
4.4. CRISPR/Cas9-Mediated Bag-1 Knockout
4.5. Proliferation/Survival Rates
4.6. MTT Cell Viability Assay
4.7. Starvation
4.8. Immunoblotting
4.9. His-Pull Down
4.10. Immunocytochemistry
4.11. In Vitro Binding Assay
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Turk, M.; Tatli, O.; Alkan, H.F.; Ozfiliz Kilbas, P.; Alkurt, G.; Dinler Doganay, G. Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction. Molecules 2021, 26, 854. https://doi.org/10.3390/molecules26040854
Turk M, Tatli O, Alkan HF, Ozfiliz Kilbas P, Alkurt G, Dinler Doganay G. Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction. Molecules. 2021; 26(4):854. https://doi.org/10.3390/molecules26040854
Chicago/Turabian StyleTurk, Miray, Ozge Tatli, Hamza Furkan Alkan, Pelin Ozfiliz Kilbas, Gizem Alkurt, and Gizem Dinler Doganay. 2021. "Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction" Molecules 26, no. 4: 854. https://doi.org/10.3390/molecules26040854