Opuntia dillenii (Ker Gawl.) Haw., Seeds Oil Antidiabetic Potential Using In Vivo, In Vitro, In Situ, and Ex Vivo Approaches to Reveal Its Underlying Mechanism of Action
Abstract
:1. Introduction
2. Results
2.1. Yield of Extraction
2.2. Acute Toxicity
2.3. Hypoglycemic Test in Non-Diabetic Rats
2.4. Antihyperglycemic Study in Nondiabetic and Diabetic Rats
2.4.1. Nondiabetic Rats
2.4.2. Diabetic Rats
2.5. Intestinal Absorption Inhibition of d-Glucose, In Situ
2.6. The ODSO Inhibits the Sodium-Dependent Absorption of d-Glucose
2.7. Inhibition Assay of Pancreatic α-Amylase Activity In Vitro
2.8. Inhibition Assay of Pancreatic α-Amylase Activity in Non-Diabetic and Diabetic Rats
2.8.1. Nondiabetic Rats
2.8.2. Diabetic Rats
2.9. Inhibition Assay of Intestinal α-Glucosidase Activity In Vitro
2.10. Kinetics of Intestinal α-Glucosidase Inhibition In Vitro
2.11. Inhibition Assay of Intestinal α-Glucosidase Activity in Nondiabetic and Diabetic Rats
2.11.1. Nondiabetic Rats
2.11.2. Diabetic Rats
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Opuntia Dillenii Fruit Harvest
4.3. Powder Preparation and Oil Extraction of Opuntia dillenii Seeds
4.4. Experimental Animals
4.5. Acute Oral Toxicity in Mice
4.6. Hypoglycemic Test in Nondiabetic Rats
4.7. Induction of Experimental Diabetes
4.8. Antihyperglycemic Study in Nondiabetic and Diabetic Rats
4.9. Single-Pass Intestinal Perfusion in Rats
4.10. Ussing Chamber Assay
4.11. Inhibition Assay of α-Amylase Activity In Vitro
4.12. Inhibition Assay of α-Amylase Activity in Normal and Diabetic Rats
4.13. Inhibition Assay of Intestinal α-Glucosidase Activity In Vitro
4.14. Kinetics of Intestinal α-Glucosidase Inhibition In Vitro
4.15. Inhibition Assay of Intestinal α-Glucosidase Activity in Normal and Diabetic Rats
4.16. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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ODSO Concentration (μg/mL) | Km (mM) | Vmax (μM/min) |
---|---|---|
0 | 71.42 | 166.66 |
165 | 71.42 | 166.66 |
325 | 71.42 | 83.3 |
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Bouhrim, M.; Ouassou, H.; Boutahiri, S.; Daoudi, N.E.; Mechchate, H.; Gressier, B.; Eto, B.; Imtara, H.; A. Alotaibi, A.; Al-zharani, M.; et al. Opuntia dillenii (Ker Gawl.) Haw., Seeds Oil Antidiabetic Potential Using In Vivo, In Vitro, In Situ, and Ex Vivo Approaches to Reveal Its Underlying Mechanism of Action. Molecules 2021, 26, 1677. https://doi.org/10.3390/molecules26061677
Bouhrim M, Ouassou H, Boutahiri S, Daoudi NE, Mechchate H, Gressier B, Eto B, Imtara H, A. Alotaibi A, Al-zharani M, et al. Opuntia dillenii (Ker Gawl.) Haw., Seeds Oil Antidiabetic Potential Using In Vivo, In Vitro, In Situ, and Ex Vivo Approaches to Reveal Its Underlying Mechanism of Action. Molecules. 2021; 26(6):1677. https://doi.org/10.3390/molecules26061677
Chicago/Turabian StyleBouhrim, Mohamed, Hayat Ouassou, Salima Boutahiri, Nour Elhouda Daoudi, Hamza Mechchate, Bernard Gressier, Bruno Eto, Hamada Imtara, Amal A. Alotaibi, Mohammed Al-zharani, and et al. 2021. "Opuntia dillenii (Ker Gawl.) Haw., Seeds Oil Antidiabetic Potential Using In Vivo, In Vitro, In Situ, and Ex Vivo Approaches to Reveal Its Underlying Mechanism of Action" Molecules 26, no. 6: 1677. https://doi.org/10.3390/molecules26061677