Polymeric Nanovectors Incorporated with Ganciclovir and HSV-tk Encoding Plasmid for Gene-Directed Enzyme Prodrug Therapy
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of Amphiphilic Prodrug Polymers
2.2. Characterization of GCV-Tagged Polymeric Micelles and Their Complexes with HSV-tk
2.3. Release of GCV from Polymeric Micelles
2.4. Characterization of HT29/HSV-tk Cells
2.5. Cytotoxicity Studies
3. Materials and Methods
3.1. Materials
3.2. Synthesis of GCV-Incorporated and MPEG-Incorporated Amphiphilic Polymers
3.3. Characterization of GCV–PCL and GCV–PCL–Chitosan
3.4. Preparation of Polymeric Prodrug Micelles
3.5. Determination of Critical Micelle Concentration
3.6. Size and Morphology of Polymeric Prodrug Micelles
3.7. Drug Release Kinetics
3.8. Establishing HSV-tk-Expressed HT29 Cells
3.9. Detection of HSV-tk Protein Expression
3.10. Development of GCV–PCL–Chitosan/HSV-tk Nanovectors
3.11. Cytotoxicity Studies
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | Mw (Da) | Mn (Da) | Polydispersity (Mw/Mn) |
---|---|---|---|
GCV–PCL | 12,996 | 11,454 | 1.13 |
GCV–PCL–chitosan | 20,354 | 17,231 | 1.18 |
Amount of DNA (µg) | Size ± SD (nm) | Zeta Potential ± SD (mV) |
---|---|---|
0 | 93.4 ± 2.5 | 38.5 ± 2.7 |
1.5 | 92.4 ± 0.9 | 37.9 ± 5.3 |
2.0 | 92.4 ± 1.2 | 37.5 ± 1.8 |
3.0 | 92.8 ± 0.7 | 30.1 ± 3.1 |
4.5 | 128.4 ± 1.5 | 28.2 ± 0.9 |
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Sawdon, A.J.; Zhang, J.; Peng, S.; Alyami, E.M.; Peng, C.-A. Polymeric Nanovectors Incorporated with Ganciclovir and HSV-tk Encoding Plasmid for Gene-Directed Enzyme Prodrug Therapy. Molecules 2021, 26, 1759. https://doi.org/10.3390/molecules26061759
Sawdon AJ, Zhang J, Peng S, Alyami EM, Peng C-A. Polymeric Nanovectors Incorporated with Ganciclovir and HSV-tk Encoding Plasmid for Gene-Directed Enzyme Prodrug Therapy. Molecules. 2021; 26(6):1759. https://doi.org/10.3390/molecules26061759
Chicago/Turabian StyleSawdon, Alicia J., Jun Zhang, Sarah Peng, Esmael M. Alyami, and Ching-An Peng. 2021. "Polymeric Nanovectors Incorporated with Ganciclovir and HSV-tk Encoding Plasmid for Gene-Directed Enzyme Prodrug Therapy" Molecules 26, no. 6: 1759. https://doi.org/10.3390/molecules26061759