Next Article in Journal
Anion–Anion Interactions in Aerogen-Bonded Complexes. Influence of Solvent Environment
Next Article in Special Issue
Constituents of Aquilaria sinensis Leaves Upregulate the Expression of Matrix Metalloproteases 2 and 9
Previous Article in Journal
Distinguished Contributions in the Fields of Biomedical and Environmental Applications Incorporating Nanostructured Materials and Composites in Journal Molecules
Previous Article in Special Issue
Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 8
10.3390/molecules26082113
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae

by
Enas Mahmoud Amer
1,†,
Saber H. Saber
2,†,
Ahmad Abo Markeb
3,
Amal A. Elkhawaga
4,
Islam M. A. Mekhemer
3,
Abdel-Naser A. Zohri
1,
Turki S. Abujamel
5,6,
Steve Harakeh
6,7,* and
Elham A. Abd-Allah
8
1
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71515, Egypt
2
Laboratory of Molecular Cell Biology, Department of Zoology, Faculty of Science, Assiut University, Assiut 71515, Egypt
3
Chemistry Department, Faculty of Science, Assiut University, Assiut 71515, Egypt
4
Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut 71515, Egypt
5
Vaccines and Immunotherapy Unit, King Fahd Medical Research Center (KFMRC), King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia
6
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia
7
Special Infectious Agents Unit, King Fahd Medical Research Center and Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia
8
Zoology Department, Faculty of Science, New Valley University, El-Kharga 72511, Egypt
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2021, 26(8), 2113; https://doi.org/10.3390/molecules26082113
Submission received: 15 February 2021 / Revised: 12 March 2021 / Accepted: 19 March 2021 / Published: 7 April 2021
(This article belongs to the Special Issue Biological and Pharmacological Activity of Plant Natural Compounds II)
Browse Figures
Review Reports Versions Notes

Abstract

Beta glucan (β-glucan) has promising bioactive properties. Consequently, the use of β-glucan as a food additive is favored with the dual-purpose potential of increasing the fiber content of food products and enhancing their health properties. Our aim was to evaluate the biological activity of β-glucan (antimicrobial, antitoxic, immunostimulatory, and anticancer) extracted from Saccharomyces cerevisiae using a modified acid-base extraction method. The results demonstrated that a modified acid-base extraction method gives a higher biological efficacy of β-glucan than in the water extraction method. Using 0.5 mg dry weight of acid-base extracted β-glucan (AB extracted) not only succeeded in removing 100% of aflatoxins, but also had a promising antimicrobial activity against multidrug-resistant bacteria, fungi, and yeast, with minimum inhibitory concentrations (MIC) of 0.39 and 0.19 mg/mL in the case of resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, respectively. In addition, AB extract exhibited a positive immunomodulatory effect, mediated through the high induction of TNFα, IL-6, IFN-γ, and IL-2. Moreover, AB extract showed a greater anticancer effect against A549, MDA-MB-232, and HepG-2 cells compared to WI-38 cells, at high concentrations. By studying the cell death mechanism using flow-cytometry, AB extract was shown to induce apoptotic cell death at higher concentrations, as in the case of MDA-MB-231 and HePG-2 cells. In conclusion, the use of a modified AB for β-glucan from Saccharomyces cerevisiae exerted a promising antimicrobial, immunomodulatory efficacy, and anti-cancer potential. Future research should focus on evaluating β-glucan in various biological systems and elucidating the underlying mechanism of action.
Keywords: Saccharomyces cerevisiae; β-glucan; antimicrobial and anticancer activities; detoxification ability; immunomodulatory effect Saccharomyces cerevisiae; β-glucan; antimicrobial and anticancer activities; detoxification ability; immunomodulatory effect

Share and Cite

MDPI and ACS Style

Mahmoud Amer, E.; Saber, S.H.; Abo Markeb, A.; Elkhawaga, A.A.; Mekhemer, I.M.A.; Zohri, A.-N.A.; Abujamel, T.S.; Harakeh, S.; Abd-Allah, E.A. Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae. Molecules 2021, 26, 2113. https://doi.org/10.3390/molecules26082113

AMA Style

Mahmoud Amer E, Saber SH, Abo Markeb A, Elkhawaga AA, Mekhemer IMA, Zohri A-NA, Abujamel TS, Harakeh S, Abd-Allah EA. Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae. Molecules. 2021; 26(8):2113. https://doi.org/10.3390/molecules26082113

Chicago/Turabian Style

Mahmoud Amer, Enas, Saber H. Saber, Ahmad Abo Markeb, Amal A. Elkhawaga, Islam M. A. Mekhemer, Abdel-Naser A. Zohri, Turki S. Abujamel, Steve Harakeh, and Elham A. Abd-Allah. 2021. "Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae" Molecules 26, no. 8: 2113. https://doi.org/10.3390/molecules26082113

APA Style

Mahmoud Amer, E., Saber, S. H., Abo Markeb, A., Elkhawaga, A. A., Mekhemer, I. M. A., Zohri, A.-N. A., Abujamel, T. S., Harakeh, S., & Abd-Allah, E. A. (2021). Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae. Molecules, 26(8), 2113. https://doi.org/10.3390/molecules26082113

Article Metrics

Back to TopTop