A New Pyrimidine Schiff Base with Selective Activities against Enterococcus faecalis and Gastric Adenocarcinoma
Abstract
:1. Introduction
2. Results
2.1. Chemistry
2.2. X-ray Structural Studies
2.3. Cells and Cytotoxicity Assay
2.3.1. Proliferation Inhibition Caused by 2 and 3
2.3.2. Morphological Changes Induced by Imine 3
2.3.3. Cell Death
2.3.4. Genotoxicity/DNA Damage
2.4. Antimicrobial Activity Assay
3. Discussion
4. Materials and Methods
4.1. Chemistry
4.2. X-ray Structural Studies
4.3. Cells and Cytotoxicity Assay
4.3.1. Chemicals
4.3.2. Cell Cultures and Reagent Preparation
4.3.3. Neutral Red Cell Viability Screening
4.3.4. Flow Cytometry
4.3.5. Microscopic Observation
4.3.6. Data Processing and Statistical Analysis
4.3.7. Annexin V/PI Staining
4.3.8. Comet Assay
4.4. Antimicrobial Activity Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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D—H⋯A | d(D—H) | d(H⋯A) | d(D⋯A) | <(D—H⋯A) |
---|---|---|---|---|
Compound 2 | ||||
N4—H4⋯N5 | 0.860 (17) | 2.277 (17) | 2.9365 (18) | 133.6 (14) |
N5—H5⋯N1i | 0.93 (2) | 2.32 (2) | 3.1358 (18) | 145.4 (14) |
C46—H46⋯N3 | 0.95 | 2.40 | 2.8894 (18) | 111 |
C43—H43⋯O5ii | 0.95 | 2.59 | 3.496 (2) | 159 |
C61—H611⋯F4iii | 0.98 | 2.54 | 3.277 (2) | 132 |
Compound 3 | ||||
N4—H4⋯N5 | 1.00 (2) | 1.75 (2) | 2.664 (2) | 150.4 (17) |
C46—H46⋯N3 | 0.95 | 2.35 | 2.918 (2) | 118 |
C25—H25⋯O5i | 0.95 | 2.53 | 3.452 (3) | 165 |
C24—H24⋯F4ii | 0.95 | 2.63 | 3.572 (2) | 171 |
C53—H53⋯F4iii | 0.95 | 2.64 | 3.298 (2) | 127 |
Symmetry codes: 2: (i) – x + 1/2, y, z − 1/2; (ii) – x + 1, − y + 1, z − 1/2; (iii) – x + 1/2, y + 1, z + 1/2; 3: (i) x + 1, y, z − 1; (ii) – x + 1, y − 1/2, − z + 1/2; (iii) − x, y − 1/2, − z + 3/2. |
Strain | Enterococcus faecalis ATCC 29212 | Streptococcus pneumonia ATCC 49619 | Staphylococcus aureus ATCC 43300 | Escherichia coli ATCC 25922 | Klebsiella pneumonia ATCC 13883 | Pseudomonas aeruginosa ATCC 27853 | Candida albicans ATCC 10231 |
---|---|---|---|---|---|---|---|
MBC (ug/mL) | |||||||
2 | 1024 | n/d | n/d | n/d | n/d | n/d | n/d |
3 | 32 | n/d | n/d | n/d | n/d | n/d | n/d |
MIC (ug/mL) | |||||||
2 | 512 | n/d | n/d | n/d | n/d | n/d | n/d |
3 | 16 | n/d | n/d | n/d | n/d | n/d | n/d |
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Stolarczyk, M.; Wolska, A.; Mikołajczyk, A.; Bryndal, I.; Cieplik, J.; Lis, T.; Matera-Witkiewicz, A. A New Pyrimidine Schiff Base with Selective Activities against Enterococcus faecalis and Gastric Adenocarcinoma. Molecules 2021, 26, 2296. https://doi.org/10.3390/molecules26082296
Stolarczyk M, Wolska A, Mikołajczyk A, Bryndal I, Cieplik J, Lis T, Matera-Witkiewicz A. A New Pyrimidine Schiff Base with Selective Activities against Enterococcus faecalis and Gastric Adenocarcinoma. Molecules. 2021; 26(8):2296. https://doi.org/10.3390/molecules26082296
Chicago/Turabian StyleStolarczyk, Marcin, Aleksandra Wolska, Aleksandra Mikołajczyk, Iwona Bryndal, Jerzy Cieplik, Tadeusz Lis, and Agnieszka Matera-Witkiewicz. 2021. "A New Pyrimidine Schiff Base with Selective Activities against Enterococcus faecalis and Gastric Adenocarcinoma" Molecules 26, no. 8: 2296. https://doi.org/10.3390/molecules26082296