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Article

Isotopic Indications of Late Pleistocene and Holocene Paleoenvironmental Changes at Boodie Cave Archaeological Site, Barrow Island, Western Australia

1
Archaeology, School of Social Science, University of Western Australia, Perth, WA 6009, Australia
2
School of Social Science, University of Queensland, Brisbane, QLD 4072, Australia
3
Australian Centre for Excellence in Biodiversity and Heritage, University of Wollongong, Wollongong, NSW 2522, Australia
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(9), 2582; https://doi.org/10.3390/molecules26092582
Submission received: 21 March 2021 / Revised: 25 April 2021 / Accepted: 26 April 2021 / Published: 28 April 2021
(This article belongs to the Special Issue Applications of Stable Isotope Analysis)

Abstract

:
This paper presents the first application of mammal tooth enamel carbonate stable isotope analysis for the purpose of investigating late Pleistocene–early Holocene environmental change in an Australian archaeological context. Stable carbon (δ13C) and oxygen (δ18O) isotope ratios were analyzed from archaeological and modern spectacled hare wallaby (Lagorchestes conspicillatus) and hill kangaroo (Osphranter robustus) tooth enamel carbonates from Boodie Cave on Barrow Island in Western Australia. δ18O results track the dynamic paleoecological history at Boodie Cave including a clear shift towards increasing aridity preceding the onset of the Last Glacial Maximum and a period of increased humidity in the early to mid-Holocene. Enamel δ13C reflects divergent species feeding ecology and may imply a long-term shift toward increasing diversity in vegetation structure. This study contributes new data to the carbonate-isotope record for Australian fauna and demonstrates the significant potential of stable isotope based ecological investigations for tracking paleoenvironment change to inter-strata resolution.

1. Introduction

Boodie Cave is located on Barrow Island in northwest Australia in an area characterized by dynamic precipitation driven by the influence of winter rainfall and tropical cyclones (Figure 1andFigure 2) [1]. Vegetation is dominated by plants utilizing the C4 photosynthetic pathway [2,3]. The archaeological site preserves rich deposits dating from the earliest period of occupation (~50 ky BP) through the post-glacial marine transgression and subsequent islandization (~7 ky BP) [4,5]. This provides a unique opportunity to reconstruct past environments and examine the complex relationships between ecological vectors and human populations during the site’s extensive history.
The notably well-preserved archaeo-fauna assemblages are diverse and contain a varying array of marine and terrestrial resources that suggest a fluctuating mosaic of habitats accompanied broader paleoenvironmental transformations in the area during the late Pleistocene and early Holocene [5,6]. However, the pursuit of further, site-specific paleoenvironmental data is fundamental to expanding understandings of influencing factors including variable precipitation, changing vegetation structure and unstable biological productivity.
Stable isotope geochemistry of archaeological animal tissue represents a well-established, high precision method for extracting local paleoecological data [7,8,9,10,11,12]. The high mineral content and crystalline characteristics of tooth enamel mean that it is highly resistant to diagenetic alteration and thus optimal for isotopic studies of Pleistocene-age archaeological deposits [13,14,15].
Utilizing established relationships linking enamel δ13C to vegetation structure and δ18O to relative humidity in macropods [16,17], this study integrates the isotopic compositions of contemporary and archaeological wallabies (Lagorchestes conspicillatus) and kangaroos (Osphranter robustus) with stratigraphic analyses to map changing paleoenvironmental conditions at Boodie Cave through time. While there have been a number of isotopic studies undertaken on modern and fossil tooth enamel of Australian macropods [18,19,20,21], this is its first application for creating a high-resolution local isotope record in an archaeological context.

2. Materials and Methods

2.1. Excavation

A total of ten 1 m × 1 m squares were excavated at Boodie Cave over the course of three field seasons between 2013 and 2015 (Figure 3) [4]. Excavation was confined to undisturbed areas and undertaken in 2–3 cm excavation units to a maximum depth of 220 cm. Location features, including stratigraphic units and in situ finds, were mapped and recorded. Excavated material was wet screened and passed through 4 mm and 2 mm nested mesh sieves.

2.2. Sampling

Archaeological (n = 56) and modern (n = 13) L. conspicillatus and O. robustus teeth were collected from the Boodie Cave archaeological site on Barrow Island Western Australia between 2013 and 2015 (Figure 4).
Archaeological specimens were identified to taxa and element at the University of Queensland School of Social Science Zooarchaeology laboratory. A modern comparative collection of several (deceased) individuals collected from Barrow Island between 2013 and 2014 facilitated identifications. All specimens were weighed and measured prior to pre-analytical processing. The archaeological material is between ~7 and 50 ka BP [4,5].

2.3. Preanatyical Processing and Pre-Treatments

Preanalytical processing was conducted at the University of Western Australian School of Social Science Archaeology laboratory in accordance with contemporary methods [13,22,23,24]. Tooth enamel from whole teeth was separated from dentine using a Micro Dremel drill fitted with diamond coated drill-bits and subsequently ground into a fine powder using an agate mortar and pestle.
Powdered tooth enamel samples were treated overnight with 50 μL of 3% hydrogen peroxide per 1.0 mg of enamel to remove organic matter. This was followed by a fifteen-minute treatment with 0.1 M acetic acid (50 μL of per 1.0 mg of sample) to remove diagenetic and absorbed carbonate. After each treatment, samples were rinsed with demineralized water, centrifuged four times, and dried in a desiccator.

2.4. Isotopic Analysis

Isotopic values are given in per mil (‰) difference in the ratio of heavier to lighter isotopes (R) compared to that of a standard and are expressed using the standard delta notion (δ) as follows: Rsample Rstandard –
δ = Rsample Rstandard 1 ×   1000
Given that the carbonate phase of macropod tooth enamel is c. 5%, approximately 6 mg samples of treated enamel (0.3 mg of carbonate) were analyzed for δ13C and δ18O. Analyses were undertaken using an GasBench II coupled with Delta XL Mass Spectrometer (Thermo-Fisher Scientific, Bremen, Germany) at the West Australian Biochemistry Centre, School of Plant Biology, University of Western Australia [25]. The isotope results were standardized to the Vienna PeeDee Belemnite (VPDB) and given in per mil (‰). Three-points normalization was used in order to reduce raw values to the international scale [26] and normalization was done based on international standards provided by IAEA: LSVEC, NBS19 and NBS18. The external error of δ13C analyses is <0.10‰ and δ18O is <0.10‰ (1 sd = standard deviation).

2.5. Statistical Analysis

Statistical visualizations and analyses were conducted using R. Pairwise Wilcoxon rank sum tests. p-values corrected for multiple comparisons are presented.

3. Results

3.1. Carbon Isotopes

Isotopic measurements were recorded for a total of 69 L. conspicillatus and O. robustus samples. All isotopic results are presented in Table S1 (Supplementary Material). Species mean isotopic results and standard deviations are summarized in Table 1. Overall δ13C values range from −9.71 ‰ to −1.11‰ (Figure 5). The adjusted p-value (0.00004) implies a statistically significant difference between the δ13C for L. conspicillatus (mean = −5.47‰, sd = 1.80) and O. robustus (mean =−3.42‰, sd =1.57). In general, late formed molars (M3 and M4) recorded higher δ13C values than those formed prior to weaning (M1 and M2) (Figure 6). This aligns with a known weaning effect [16].
Stratigraphic units, and corresponding chronological, paleoenvironmental and archeological contexts for Boodie Cave are documented in recent syntheses and summarized in Table 2 [4,5,27]. Comparison did not identify a statistically discernible separation between the archaeological and modern teeth in this study (Figure 7). Equally, no significant inter-strata differences were identified. This is true even when a −1.2‰ correction is added to the archaeological specimens to account for depletion in modern δ13C resulting from burning of fossil fuels [28,29]. While no statistically significant differences were detected between stratigraphic units, δ13C associated with the islandization phase trended more negative than signals recorded in layers linked to regression of the shoreline with falling sea levels.

3.2. Oxygen Isotopes

Measured δ18O is statistically indistinguishable between L. conspicillatus (mean = 0.01‰, sd = 1.33) and O. robustus (mean = −0.12‰, sd = 0.93) (Table 1). Overall δ18O values range from −2.94‰ to 4.30‰ (Figure 5). This range overlaps the known modern δ18O values for Macropus spp. and Osphranter spp. in the wider Carnarvon bioregion (−2.07 to 5.62‰) [17]. Inter-tooth comparison did not identify any notable trends (Figure 8).
Inter-stratigraphic analysis identified several significant differences in δ18O. Stratigraphic units six (mean = 0.10‰, sd = 0.42) and seven (mean = 0.52‰, sd = 0.82) are both statistically distinct from the modern specimens (mean = −0.375 ‰, sd = 0.52) (adjusted p-values = 0.86; 0.88) (Figure 9). More broadly, when stratigraphic units were analyzed by chronological context, Late Pleistocene samples pre-dating the Last Glacial Maximum (LGM) (mean = 0.53‰, sd = 0.72) were significantly different when compared to the post LGM Holocene group (mean = −0.17‰, sd = 1.37) (adjusted p-value = 0.08) (Figure 10). Similarly, a statistically notable shift was recorded between modern (mean = −0.38‰, sd = 0.52) and the pre-LGM samples (mean = 0.53‰, sd = 0.72) (adjusted p-value = 0.01).

4. Discussion

4.1. Feeding Ecology and Paleo-Vegetation

Based on the measured enamel δ13C and the known fractionation factor of approximately 12‰ [16], dietary δ13C for macropods in the study ranged from −21.71‰ to −13.11‰. This implies that the sampled taxa subsisted on a mixed C3 and C4 diet (Table 3) [30,31]. Notably, a clear separation is evident between L. conspicillatus and O. robustus. Although this difference may be influenced by the smaller number of O. robustus samples, it more likely reflects a dietary divergence. While both subsist on a predominantly C4 diet, results suggest that L. conspicillatus incorporates more C3 into its diet.
Given that 70% of Barrow Island, including the area surrounding Boodie Cave, is characterized by limestone uplands dominated by C4 Triodia wiseana [3], this is interpreted to represent divergent behavioral and feeding strategies rather than distinct habitats. In particular, larger kangaroos species, including O. robustus, are widely regarded as preferential grazers that intermittently revert to browsing in arid habitats [32,33,34,35].
Conversely, although less is known about the feeding ecology of L. conspicillatus, it is understood that it is a selective feeder prone to browse on a mixture of monocotyledonous and dicotyledonous species including the tips of Triodia spp., shrub foliage and dicot herbs [36,37,38,39].
While no statistically significant differences were detected between archaeological strata, a number of noteworthy features and trends were identified in the data. In particular, L. conspicillatus δ13C associated with the island and islandization phases trended more negative than signals recorded in layers linked to regression of the shoreline, while O. robustus signals were more positive in the transgression phase. That is, there is a reversal in the direction of change between species. Given that the island and islandization phases are associated with a climatic period broadly defined by ameliorated conditions (including warmer temperatures, increased precipitation, and improved biological productivity) [27,40,41], it is posited that macropods had access to a wider range of lush vegetation and therefore their opposing tendencies toward graze and browse based diets were magnified due to these environmental shifts.
Even when inter-species difference is taken into account, it is clear that δ13C recorded for modern teeth and enamel from more recent archaeological strata (SU1-3) are highly variable compared to earlier pre-LGM layers. Although the noisier signal can be partially attributed to sample size, it is probable that it reflects increased diversity in diet and potentially also in vegetation structure. This is likely the result of heightened variation in biological productivity in the arid zone during the Holocene and increases in sub annual fluctuations in rainfall at the onset of Marine Isotope Stage (MSI) 1 [27,40,42]. Given that C3 and C4 plants have different sensitivity to water availability, diversity of diet also likely contributed to variability in Holocene δ18O.

4.2. Paleohumidity

The lack of discernible statistical difference between δ18O for L. conspicillatus and O. robustus supports the expectation that the taxa are utilizing comparable water sources to meet their hydration requirements. Given the lack of free water sources on Barrow Island [43], it is hypothesized that the non-obligate drinkers are primarily obtaining hydration from dietary plant matter [39,44]. In addition, the lack of discernible difference between teeth aligns with existing data implying the absence of a weaning effect for δ18O and allows for species and molars to be analyzed in bulk [17].
δ18O exhibited statistically significant separation between modern samples and the strata associated with the pre-LGM regressing shoreline (SU 6 and SU 7) between 36.6 and 46.2 ka BP. On the basis that the vast majority of physiological and environmental factors that may influence the signal are negated by the environment, this difference is interpreted to reflect changes in relative humidity.
Broadly, the higher δ18O associated with late Pleistocene samples implies drier conditions and while contradicting some existing continental and regional models that suggest the late MIS 3 between 40 and 30 ka was predominantly characterized by a humid phase and wetter conditions than the present [27,45], a more recent synthesis by De Deckker et al. 2020 [46] is consistent with significantly increasing aridity leading into the LGM. Conditions were highly variable across the continent and the onset of increasingly arid and cool conditions has been noted in the northern Australia from 35 ka [41]. This variability attests to the relevance of seeking proximal archaeological sources for interpreting paleoenvironments.
In addition, Holocene (post-LGM) δ18O was significantly more negative than the late Pleistocene samples. Given that many Holocene samples are derived from stratigraphic unit three and date to between 6.8 and 7.2 ka BP, it is probable that this result aligns with the earliest Holocene low δ18O values identified in the regional Cape Range stalagmite record, coinciding with southern Australia mega-lake highstands [1]. Significantly, the archaeology in this period is exceptionally rich indicating intensive site use associated with a humid phase [4]
Importantly, results do not appear to reflect the interaction between mean temperature and relative humidity established by Murphy et al. 2007 [17] but are more strongly linked to known changes in levels of humidity [27,41]

5. Conclusions

Paleoenvironmental signatures in archaeological and modern teeth from the Boodie Cave archaeological site on Barrow Island track shifting climatic conditions and, to a lesser extent, evolving habitats. Shifting δ13C potentially implies a slow but progressive increase in vegetation diversity associated with broader climatic amelioration.
Significant inter-strata variation in δ18O between modern, Holocene, and late Pleistocene samples broadly aligns with continental and regional models but also contributes to a locate climate record. High δ18O values between 36.6 and 46.2 ka BP demonstrates the local onset of pre-LGM aridity and an early to mid-Holocene low in δ18O aligns with an increasingly humid period reflected in regional speleothem records, consistent with existing climate models [1,46].
Fundamentally, this study demonstrates for the first time that isotopic analysis of macropod tooth enamel can be used to track paleoenvironmental shifts to inter-strata resolution in Australian archaeological contexts.

Supplementary Materials

The following is available online: Table S1: Isotopic results for L. conspicillatus and O. robustus.

Author Contributions

Conceptualization, methodology, analysis, and original draft, J.S.; taxa identification, T.M.; review and supervision, T.M. and P.V. All authors have read and agreed to the published version of the manuscript.

Funding

This research was conducted as part of the Barrow Island Archaeological Project and was funded by an ARC Discovery Grant (DP130100802) 2013–2015 awarded to Peter Veth, Tiina Manne, Alistair Paterson, Mark Basgall, David Zeanah and Christa Placzek. Manne’s work was also funded by an ARC Discovery Early Career Researcher Award (DE150101597).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

Department of Parks and Wildlife (now Department of Biodiversity Conservation and Attractions), WA Oil, and Chevron Australian Business Unit provided personnel and logistical support. Greg Skrzyrpek and Douglas Ford from the West Australian Biochemistry Centre at The University of Western Australia are thanked for technical advice and practical support relating to sample preparation and analyses. Claire Garcia-Webb is acknowledged for assistance with site plan illustration.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Sample Availability

Samples of the compounds are not available from the authors.

References

  1. Denniston, R.F.; Asmerom, Y.; Lachniet, M.; Polyak, V.J.; Hope, P.; An, N.; Rodzinyak, K.; Humphreys, W.F. A Last Glacial Maximum through middle Holocene stalagmite record of coastal Western Australia climate. Quat. Sci. Rev. 2013, 77, 101–112. [Google Scholar] [CrossRef]
  2. Hattersley, P.W. The distribution of C3 and C4 grasses in Australia in relation to climate. Oecologia 1983, 57, 113–128. [Google Scholar] [CrossRef]
  3. Short, S.; Turner, B. Distribution and Abundance of Spectacled Hare-wallabies and Euros on Barrow Island, Western Australia. Wildl. Res. 1991, 18, 421–429. [Google Scholar] [CrossRef]
  4. Veth, P.; Kendrick, P.; Ward, I.; Manne, T.; Ulm, S.; Ditchfeild, K.; Dortch, J.; Hook, F.; Petchey, F.; Hogg, A.; et al. Early human occupation of a Maritime Desert, Barrow Island, North-West Australia. Quat. Sci. Rev. 2017, 168, 19–29. [Google Scholar] [CrossRef]
  5. Ward, I.; Veth, P.; Prossor, L.; Denham, T.; Ditchfeild, K.; Manne, T.; Kendrick, P.; Byrne, C.; Hook, F.; Troitzsch, U. 50,000 years of archaeological site stratigraphy and micromorphology in Boodie Cave, Barrow Island, Western Australia. J. Archaeol. Sci. Rep. 2017, 15, 344–369. [Google Scholar] [CrossRef]
  6. Manne, T.; Veth, P.M. Late Pleistocene and early Holocene exploitation of estuarine communities in northwestern Australia. Quat. Int. 2015, 385, 112–123. [Google Scholar] [CrossRef]
  7. Balasse, M.; Ambrose, S. The seasonal mobility model for prehistoric herders in the south-western cape of south Africa assessed by isotopic analysis of sheep tooth enamel. J. Archaeol. Sci. 2002, 29, 917–932. [Google Scholar] [CrossRef]
  8. Balasse, M.; Smith, A.B.; Ambrose, S.H.; Leigh, S.R. Determining sheep birth seasonality by analysis of tooth enamel oxygen isotope ratios: The late Stone Age site of Kasteelberg (South Africa). J. Archaeol. Sci. 2003, 30, 205–215. [Google Scholar] [CrossRef]
  9. Cerling, T.E.; Harris, J.M. Carbon isotope fractionation between diet and bioapatite in ungulate mammals and implications for ecological and paleoecological studies. Oecologia 1999, 120, 347–363. [Google Scholar] [CrossRef] [PubMed]
  10. Clementz, M.T. New Insights from Old Bones: Stable Isotope Analysis of Fossil Mammals. Am. Soc. Mammal. 2012, 93, 368–380. [Google Scholar] [CrossRef]
  11. Fraser, R.A.; Grun, R.; Privat, K.; Gagan, M.K. Stable-isotope microprofiling of wombat tooth enamel records seasonal changes in vegetation and environmental conditions in eastern Australia. Palaeogeogr. Palaeoclimatol. Palaeoecol. 2008, 269, 66–77. [Google Scholar] [CrossRef]
  12. Pilaar Birch, S.E. Stable isotopes in zooarchaeology: An introduction. Archaeol. Anthropol. Sci. 2013, 5, 81–83. [Google Scholar] [CrossRef] [Green Version]
  13. Balasse, M. Reconstructing dietary and environmental history from enamel isotopic analysis: Time resolution of intra-tooth sequential sampling. Int. J. Osteoarchaeol. 2002, 12, 155–165. [Google Scholar] [CrossRef]
  14. Koch, P. Isotopic Studies of the Biology of Modern and Fossil Vertebrates. In Stable Isotopes in Ecology and Environmental Science; Michener, R., Lajtha, K., Eds.; Blackwell Publishing: Oxford, UK, 2007; pp. 99–154. [Google Scholar]
  15. Wang, Y.; Cerling, T.E. A model of fossil tooth and bone diagenesis: Implications for paleodiet reconstruction from stable isotopes. Palaeogeogr. Palaeoclimatol. Palaeoecol. 1994, 107, 281–289. [Google Scholar] [CrossRef]
  16. Murphy, B.P.; Bowman, D.M.J.S.; Gagan, M.K. Sources of carbon isotope variation in kangaroo bone collagen and tooth enamel. Geochim. Cosmochim. Acta 2007, 71, 3847–3858. [Google Scholar] [CrossRef]
  17. Murphy, B.P.; Bowman, D.M.J.S.; Gagan, M.K. The interactive effect of temperature and humidity on the oxygen isotope composition of kangaroos. Funct. Ecol. 2007, 21, 757–766. [Google Scholar] [CrossRef]
  18. Prideaux, G.J.; Long, J.A.; Ayliffe, L.K.; Hellstrom, J.C.; Pillans, B.; Boles, W.E.; Hutchinson, M.N.; Roberts, R.G.; Cupper, M.L.; Arnold, L.J.; et al. An arid-adapted middle Pleistocene vertebrate fauna from south-central Australia. Nature 2007, 445, 422–425. [Google Scholar] [CrossRef]
  19. Forbes, M.S.; Kohn, M.J.; Bestland, E.A.; Wells, R.T. Late Pleistocene environmental change interpreted from δ13C and δ18O of tooth enamel from the Black Creek Swamp Megafauna site, Kangaroo Island, South Australia. Palaeogeogr. Palaeoclimatol. Palaeoecol. 2010, 291, 319–327. [Google Scholar] [CrossRef]
  20. Brookman, T.H.; Ambrose, S.H. Seasonal variation in kangaroo tooth enamel oxygen and carbon isotopes in southern Australia. Quat. Res. 2012, 78, 256–265. [Google Scholar] [CrossRef]
  21. Montanari, S.; Louys, J.; Price, G. Pliocene paleoenvironments of Southeastern Queensland, Australia inferred from stable isotopes of marsupial tooth enamel. PLoS ONE 2013, 8, e66221. [Google Scholar] [CrossRef] [Green Version]
  22. Garvie-Lok, S.J.; Varney, T.L.; Katzenberg, M.A. Preparation of bone carbonate for stable isotope analysis: The effects of treatment time and acid concentration. J. Archaeol. Sci. 2004, 31, 763–776. [Google Scholar] [CrossRef]
  23. Koch, P.L.; Tuross, N.; Fogel, M.L. The effects of sample treatment and diagenesis on the isotopic integrity of carbonate in biogenic hydroxylapatite. J. Archaeol. Sci. 1997, 24, 417–429. [Google Scholar] [CrossRef]
  24. Skippington, J.; Veth, P.; Manne, T.; Slack, M. Preanalytical processing of archaeological mammal enamel apatite carbonates for stable isotope investigations: A comparative analysis of the effect of acid treatment on samples from Northwest Australia. International. Int. J. Osteoarchaeol. 2019, 29, 760–771. [Google Scholar] [CrossRef]
  25. Paul, D.; Skrzypek, G. Assessment of carbonate-phosphoric acid analytical technique performed using GasBench II in continuous flow isotope ratio mass spectrometry. Int. J. Mass Spectrom. 2007, 262, 180–186. [Google Scholar] [CrossRef]
  26. Skrzypek, G. Normalization procedures and reference material selection in stable HCNOS isotope analyses—An overview. Anal. Bioanal. Chem. 2013, 405, 2815–2823. [Google Scholar] [CrossRef]
  27. Fitzsimmons, K.E.; Cohen, T.J.; Hesse, P.P.; Jansen, J.; Nanson, G.C.; May, J.; Barrows, T.T.; Haberlah, D.; Hilgers, A.; Kelly, T.; et al. Late Quaternary palaeoenvironmental change in the Australian drylands. Quat. Sci. Rev. 2013, 74, 78–96. [Google Scholar] [CrossRef]
  28. Friedli, H.; Loetscher, H.; Oeschger, H.; Siegenthaler, U.; Stauffer, B. Ice core record of the 13C/12C ratio of atmospheric CO2 in the past two centuries. Nature 1986, 324, 237–238. [Google Scholar] [CrossRef]
  29. Leuenberger, M.; Siegenthaler, U.; Langway, C. Carbon isotope compositionof atmospheric CO2 during the last ice age from an Antarctic ice core. Nature 1992, 357, 488–490. [Google Scholar] [CrossRef]
  30. O’Leary, M. Carbon isotopes in photosynthesis. Bioscience 1988, 38, 328–336. [Google Scholar] [CrossRef]
  31. Johnson, B.J.; Miller, G.H.; Fogel, M.L.; Beaumont, P.B. The determination of late Quaternary paleoenvironments at Equus Cave, South Africa, using stable isotopes and amino acid racemization in ostrich eggshell. Palaeogeog. Palaeoclim. Palaeoecol. 1997, 136, 121–137. [Google Scholar] [CrossRef]
  32. Ealey, E.H.M. Ecology of the Euro, Macropus robustus (Gould) in north-western Australia: Age and growth. CSIRO Wildl. Res. 1967, 12, 67–80. [Google Scholar] [CrossRef]
  33. Hume, I. Digestive Physiology and Nutrition of Marsupial; Cambridge University Press: London, UK, 1982. [Google Scholar]
  34. Jackson, S. Australian Mammals: Biology and Captive Management; CSIRO Publishing: Collingwood, Australia, 2003. [Google Scholar]
  35. Jackson, S.; Groves, C. Taxonomy of Australian Mammals; CSIRO Publishing: Clayton, Australia, 2015. [Google Scholar]
  36. Main, A.R.; Yadav, M. Conservation of macropods in reserves in Western Australia. Biol. Conserv. 1971, 3, 123–133. [Google Scholar] [CrossRef]
  37. Burbidge, A.A.; Johnson, K.A. Spectacled Hare-wallaby, Lagorchestes conspicillatus. In Complete Book of Australian Mammals; Strahan, R., Ed.; Angus and Robertson: Sydney, Australia, 1983; pp. 197–198. [Google Scholar]
  38. Lngleby, S.; Westoby, M. Habitat Requirements of the Spectacled Hare-wallaby (Lagorchestes conspicillatus) in the Northern Territory and Western Australia. Wildl. Res. 1992, 19, 721–741. [Google Scholar] [CrossRef]
  39. Strahan, R. (Ed.) The Mammals of Australia; Reed Books: Chatswood, Australia, 1995. [Google Scholar]
  40. Morton, S.R.; Smith, D.M.S.; Dickman, C.R.; Dunkerley, D.L.; Friedel, M.H.; McAllister, R.R.J.; Reid, J.R.W.; Roshier, D.A.; Smith, M.A.; Walsh, F.J.; et al. A fresh framework for the ecology of arid Australia. J. Arid Environ. 2011, 75, 313–329. [Google Scholar] [CrossRef]
  41. Williams, A.N.; Veth, P.; Steffan, W.; Ulm, S.; Turney, C.S.; Reeves, J.M.; Phipps, S.J.; Smith, M. A continental narrative: Human settlement patterns and Australian climate change over the last 35,000 years. Quat. Sci. Rev. 2015, 28, 91–112. [Google Scholar] [CrossRef]
  42. van Etten, E.J.B. Inter-annual rainfall variability of arid Australia: Greater than elsewhere? Aust. Geogr. 2009, 40, 109–120. [Google Scholar] [CrossRef]
  43. King, J.M.; Bradshaw, S.D. Stress in an island kangaroo? The Barrow Island euro, Macropus robustus isabellinus. Gen. Comp. Endocrinol. 2010, 167, 60–67. [Google Scholar] [CrossRef]
  44. Ayliffe, L.K.; Chivas, A.R. Oxygen isotope composition of the bone phosphate of Australian kangaroos: Potential as a palaeoenvironmental recorder. Geochim. Cosmochim. 1990, 54, 2603–2609. [Google Scholar] [CrossRef]
  45. Veth, P.; Smith, M.; Bowler, J.M.; Fitzsimmons, K.E.; Williams, A.; Hiscock, P. Excavations at Parnkupirti, Lake Gregory, Great Sandy Desert: OSL ages for occupation before the Last Glacial Maximum. Aust. Archaeol. 2009, 69, 1–10. [Google Scholar] [CrossRef]
  46. De Deckker, P.; Moros, M.; Perner, K.; Blanz, T.; Wacker, L.; Schneider, R.; Barrows, T.T.; O’Loingsigh, T.; Jansen, E. Climatic evolution in the Australian region over the last 94 ka—Spanning human occupancy—And unveiling the Last Glacial Maximum. Quat. Sci. Rev. 2020, 249, 106593. [Google Scholar] [CrossRef]
Figure 1. Location of Boodie Cave, Barrow Island, Western Australia.
Figure 1. Location of Boodie Cave, Barrow Island, Western Australia.
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Figure 2. Photograph of Boodie Cave highlighting southwest entrance (Photograph by Peter Veth).
Figure 2. Photograph of Boodie Cave highlighting southwest entrance (Photograph by Peter Veth).
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Figure 3. Site plan showing location of squares.
Figure 3. Site plan showing location of squares.
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Figure 4. Examples of archaeological macropod teeth used for isotopic analysis from Boodie Cave: (a) right mandible fragment of O. robustus containing P3, M1, M2, M3 (from square G100, stratigraphic unit 3, excavation unit 510); (b) left maxilla fragments of O. robustus containing P3, M1, M2, M3 and M4 (from square G101, stratigraphic unit 3, excavation unit 534); (c) left mandible fragment of L. conspicillatus containing M2 and M3 (from square G101, stratigraphic unit 3, excavation unit 534); (d) left mandible fragment of L. conspicillatus containing M2, M3, and M4 (from square F101, stratigraphic unit 3, excavation unit 409).
Figure 4. Examples of archaeological macropod teeth used for isotopic analysis from Boodie Cave: (a) right mandible fragment of O. robustus containing P3, M1, M2, M3 (from square G100, stratigraphic unit 3, excavation unit 510); (b) left maxilla fragments of O. robustus containing P3, M1, M2, M3 and M4 (from square G101, stratigraphic unit 3, excavation unit 534); (c) left mandible fragment of L. conspicillatus containing M2 and M3 (from square G101, stratigraphic unit 3, excavation unit 534); (d) left mandible fragment of L. conspicillatus containing M2, M3, and M4 (from square F101, stratigraphic unit 3, excavation unit 409).
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Figure 5. δ18O and δ13C for L. conspicillatus and O. robustus.
Figure 5. δ18O and δ13C for L. conspicillatus and O. robustus.
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Figure 6. Box plot L. conspicillatus and O. robustus δ13C by tooth (M = molar, P = premolar).
Figure 6. Box plot L. conspicillatus and O. robustus δ13C by tooth (M = molar, P = premolar).
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Figure 7. Box plot L. conspicillatus and O. robustus δ13C by stratigraphic unit.
Figure 7. Box plot L. conspicillatus and O. robustus δ13C by stratigraphic unit.
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Figure 8. Box plot L. conspicillatus and O. robustus δ18O by tooth (M = molar, P = premolar).
Figure 8. Box plot L. conspicillatus and O. robustus δ18O by tooth (M = molar, P = premolar).
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Figure 9. Box plot L. conspicillatus and O. robustus δ18O by stratigraphic unit.
Figure 9. Box plot L. conspicillatus and O. robustus δ18O by stratigraphic unit.
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Figure 10. Box plot L. conspicillatus and O. robustus δ18O by climatic period.
Figure 10. Box plot L. conspicillatus and O. robustus δ18O by climatic period.
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Table 1. Mean stable carbon and oxygen data for modern and archaeological L. conspicillatus and 2O. robustus tooth enamel carbonates.
Table 1. Mean stable carbon and oxygen data for modern and archaeological L. conspicillatus and 2O. robustus tooth enamel carbonates.
SpeciesTypenδ13C 1sdδ18O 1sd
L. conspicillatusArchaeological40−5.221.74−0.071.45
Modern 9−6.581.70−0.340.49
O. robustusArchaeological16−3.461.76−0.040.99
Modern 4−3.280.41−0.450.68
1 All δ13C and δ18O values given in per mil [‰, VPDB]. The external error of δ13C analyses is < 0.10‰ and the external error of δ18O is < 0.10‰ (1 sd). 2 sd = standard deviation.
Table 2. Stratigraphic unit and corresponding chronological and paleoenvironmental context.
Table 2. Stratigraphic unit and corresponding chronological and paleoenvironmental context.
Stratigraphic Unit Chronology (ka BP) 1Local Paleoenvironmental
Context 1
Regional Climate
Context 2
11.7–2.5Fully marine (island)MIS1, Holocene variability
22.5−6.8Fully marine (island)
36.8–7.2Islandization
47.2–7.4Islandization
57.4–22.4Transgressing shorelineStart of MIS1 at 14 ka, deglacial variability
DiscontinuityMIS2, LGM aridity and complex glacial response
636.6–42.6Regressing ShorelineLate MIS3, humid phase, and initiation of cooler conditions
742.6–46.2Regressing Shoreline
DiscontinuityMIS3
846.2–51.1Transgressing Shoreline; cave opens MIS3
9~77Continental–regressing shoreline; cave closedMIS4
1 Based on existing archaeological and paleoenvironmental syntheses of Boodie Cave [4,5]; 2 Based on synthesis of existing paleoenvironmental data from Australian continent [27].
Table 3. δ13Cenamel, δ13Cdiet and %C3 diet for archaeological and modern taxa.
Table 3. δ13Cenamel, δ13Cdiet and %C3 diet for archaeological and modern taxa.
SpeciesType δ13Cenamelδ13Cdiet 1 %C3 Diet 2
L. conspicillatusArchaeological−5.22−17.2223.00%
Modern−6.58−18.5832.71%
O. robustusArchaeological−3.46−15.4610.43%
Modern−3.28−15.289.14%
1 δ13Cdiet is calculated by subtracting the known diet-enamel enrichment factor of 12‰ from δ13Cenamel.; 2 %C3 diet in calculated using the following mixing equation: f1 = (δSAMPLE-δSOURCE2)/(δsOURCE1-δSOURCE2) × 100 where source 1 is the average δ13C for C3 vegetation (−28‰) and source 2 is the average δ13C for C4 (−14‰) [30,31].
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Skippington, J.; Manne, T.; Veth, P. Isotopic Indications of Late Pleistocene and Holocene Paleoenvironmental Changes at Boodie Cave Archaeological Site, Barrow Island, Western Australia. Molecules 2021, 26, 2582. https://doi.org/10.3390/molecules26092582

AMA Style

Skippington J, Manne T, Veth P. Isotopic Indications of Late Pleistocene and Holocene Paleoenvironmental Changes at Boodie Cave Archaeological Site, Barrow Island, Western Australia. Molecules. 2021; 26(9):2582. https://doi.org/10.3390/molecules26092582

Chicago/Turabian Style

Skippington, Jane, Tiina Manne, and Peter Veth. 2021. "Isotopic Indications of Late Pleistocene and Holocene Paleoenvironmental Changes at Boodie Cave Archaeological Site, Barrow Island, Western Australia" Molecules 26, no. 9: 2582. https://doi.org/10.3390/molecules26092582

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