Human Serum Albumin Labelling with a New BODIPY Dye Having a Large Stokes Shift
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry
2.2. Bioconjugation
Synthesis of HSA-HcyTFAc-BODIPY Conjugate
2.3. In Vitro Cytotoxicity Assay
3. Materials and Methods
3.1. BODIPY Compound 8
3.2. BODIPY-COOH 1
3.3. BODIPY Compound 11
3.4. BODIPY-Maleimide 12
3.5. HSA-HcyTFAc
3.6. HSA-HcyTFAc-BODIPY
3.7. In Vitro Cytotoxicity Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Compound | λabs (nm) | λem (nm) | log ε | ∆λ (nm) |
---|---|---|---|---|
BODIPY 1 | 580 | 678 | 4.54 | 98 |
BODIPY 11 | 528 | 662 | 4.32 | 134 |
Conditions | HSA Type | Higher Aggregates | Monomer~66.5 kDa |
---|---|---|---|
HSA | 23.5 | 76.5 | |
Without DTT | HSA-HcyTFAc | 57.2 | 42.8 |
HSA-HcyTFAc-BODIPY | 66.7 | 33.3 | |
With DTT (data not shown) | HSA | 16.5 | 83.5 |
HSA-HcyTFAc | 15.4 | 84.6 | |
HSA-HcyTFAc-BODIPY | 14.8 | 85.2 |
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Raskolupova, V.I.; Popova, T.V.; Zakharova, O.D.; Nikotina, A.E.; Abramova, T.V.; Silnikov, V.N. Human Serum Albumin Labelling with a New BODIPY Dye Having a Large Stokes Shift. Molecules 2021, 26, 2679. https://doi.org/10.3390/molecules26092679
Raskolupova VI, Popova TV, Zakharova OD, Nikotina AE, Abramova TV, Silnikov VN. Human Serum Albumin Labelling with a New BODIPY Dye Having a Large Stokes Shift. Molecules. 2021; 26(9):2679. https://doi.org/10.3390/molecules26092679
Chicago/Turabian StyleRaskolupova, Valeria I., Tatyana V. Popova, Olga D. Zakharova, Anastasia E. Nikotina, Tatyana V. Abramova, and Vladimir N. Silnikov. 2021. "Human Serum Albumin Labelling with a New BODIPY Dye Having a Large Stokes Shift" Molecules 26, no. 9: 2679. https://doi.org/10.3390/molecules26092679