Model Systems for Evidencing the Mediator Role of Riboflavin in the UVA Cross-Linking Treatment of Keratoconus
Abstract
:1. Introduction
2. Results and Discussion
2.1. Rheological Measurements
2.2. STEM Images
2.3. MicroDSC Measurements
2.4. Radical Species Formed in Collagen/Riboflavin/UVA Systems
3. Materials and Methods
3.1. Collagen Extraction and Purification
3.2. Sample Preparation
3.3. Instruments
3.3.1. STEM
3.3.2. Rheometry
3.3.3. MicroDSC
3.3.4. EPR Spectroscopy
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Sample | Collagen 0.25% | Riboflavin 0.1% | Hyaluronic Acid 0.1% | UVA Exposure |
---|---|---|---|---|
1 | x | - | - | - |
1a | x | - | - | x |
2 | x | x | - | - |
2a | x | x | - | x |
3 | x | - | x | - |
3a | x | - | x | x |
4 | x | x | x | - |
4a | x | x | x | x |
Sample | 10 °C | 20 °C | 25 °C | 37 °C (1 h) | 37 °C (2 h) | 37 °C (3 h) |
---|---|---|---|---|---|---|
1 | 0.79 | 0.57 | 0.36 | 9.90 | 43.43 | 63.88 |
1a | 0.66 | 0.66 | 0.53 | 14.16 | 77.04 | 149.10 |
2 | 0.77 | 0.49 | 0.34 | 40.08 | 72.16 | 113.26 |
2a | 0.60 | 0.39 | 0.47 | 1.38 | 19.36 | 45.00 |
3 | 0.67 | 0.66 | 0.42 | 62.39 | 104.70 | 137.52 |
3a | 0.51 | 0.51 | 0.43 | 41.11 | 75.05 | 94.90 |
4 | 0.59 | 0.49 | 0.24 | 49.88 | 50.11 | 52.88 |
4a | 0.61 | 0.52 | 0.43 | 8.28 | 46.73 | 71.83 |
Sample | Tpeak1, °C | Tpeak2, °C | Tpeak3, °C | Denaturation Heat, J/g |
---|---|---|---|---|
1 (pH 3.5) | 36.10 | 41.06 | 45.82 | 0.27 |
1 (pH 7.5) | 36.74 | 43.93 | 47.15 | 0.29 |
Sample | Tpeak1, °C | Tpeak2, °C | Tpeak3, °C | Tpeak4, °C | Denaturation Heat, J/g |
---|---|---|---|---|---|
1 | 36.74 | 43.93 | 47.15 | - | 0.23 |
1a | 37.22 | 44.15 | 47.42 | - | 0.27 |
2 | 37.60 | 43.89 | 50.32 | - | 0.29 |
2a | 42.44 | 43.88 | 54.24 | 57.33 | 0.24 |
3 | 32.96 | 40.31 | 43.71 | 50.95 | 0.17 |
3a | 40.80 | 43.70 | 50.63 | 54.04 | 0.13 |
4 | 37.46 | 43.93 | 51.43 | 54.15 | 0.27 |
4a | 38.73 | 44.04 | 50.43 | - | 0.26 |
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Constantin, M.M.; Corbu, C.G.; Mocanu, S.; Popescu, E.I.; Micutz, M.; Staicu, T.; Şomoghi, R.; Trică, B.; Popa, V.T.; Precupas, A.; et al. Model Systems for Evidencing the Mediator Role of Riboflavin in the UVA Cross-Linking Treatment of Keratoconus. Molecules 2022, 27, 190. https://doi.org/10.3390/molecules27010190
Constantin MM, Corbu CG, Mocanu S, Popescu EI, Micutz M, Staicu T, Şomoghi R, Trică B, Popa VT, Precupas A, et al. Model Systems for Evidencing the Mediator Role of Riboflavin in the UVA Cross-Linking Treatment of Keratoconus. Molecules. 2022; 27(1):190. https://doi.org/10.3390/molecules27010190
Chicago/Turabian StyleConstantin, Mihaela Monica, Cătălina Gabriela Corbu, Sorin Mocanu, Elena Irina Popescu, Marin Micutz, Teodora Staicu, Raluca Şomoghi, Bogdan Trică, Vlad Tudor Popa, Aurica Precupas, and et al. 2022. "Model Systems for Evidencing the Mediator Role of Riboflavin in the UVA Cross-Linking Treatment of Keratoconus" Molecules 27, no. 1: 190. https://doi.org/10.3390/molecules27010190