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Article

The Effect of Deoxyfluorination on Intermolecular Interactions in the Crystal Structures of 1,6-Anhydro-2,3-epimino-hexopyranoses

by
Martin Jakubec
1,
Ivana Císařová
2,
Jindřich Karban
1 and
Jan Sýkora
1,*
1
Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, v. v. i., Rozvojová 135, 165 02 Prague, Czech Republic
2
Department of Inorganic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 2030, 128 40 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(1), 278; https://doi.org/10.3390/molecules27010278
Submission received: 10 December 2021 / Revised: 28 December 2021 / Accepted: 30 December 2021 / Published: 3 January 2022
(This article belongs to the Special Issue Intermolecular Interactions in Crystal Lattice)
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Abstract

:
The effect of substitution on intermolecular interactions was investigated in a series of 1,6-anhydro-2,3-epimino-hexopyranoses. The study focused on the qualitative evaluation of intermolecular interactions using DFT calculations and the comparison of molecular arrangements in the crystal lattice. Altogether, ten crystal structures were compared, including two structures of C4-deoxygenated, four C4-deoxyfluorinated and four parent epimino pyranoses. It was found that the substitution of the original hydroxy group by hydrogen or fluorine leads to a weakening of the intermolecular interaction by approximately 4 kcal/mol. The strength of the intermolecular interactions was found to be in the following descending order: hydrogen bonding of hydroxy groups, hydrogen bonding of the amino group, interactions with fluorine and weak electrostatic interactions. The intermolecular interactions that involved fluorine atom were rather weak; however, they were often supported by other weak interactions. The fluorine atom was not able to substitute the role of the hydroxy group in molecular packing and the fluorine atoms interacted only weakly with the hydrogen atoms located at electropositive regions of the carbohydrate molecules. However, the fluorine interaction was not restricted to a single molecule but was spread over at least three other molecules. This feature is a base for similar molecule arrangements in the structures of related compounds, as we found for the C4-Fax and C4-Feq epimines presented here.

1. Introduction

Fluorinated carbohydrates are members of a large family of glycomimetics—chemically modified carbohydrates that structurally and functionally mimic natural carbohydrate ligands [1,2,3,4]. Fluorine features a similar van der Waals atomic radius and electronegativity to oxygen and thus can effectively mimic a hydroxyl group [5,6,7]. The replacement of a hydroxyl group by fluorine (deoxyfluorination) in a carbohydrate results in minimal steric alterations with respect to the parent molecule while affecting electron density, lipophilicity, glycosidic bond stability and other properties of the original carbohydrate molecule [8]. One of the most important changes is the loss of the hydrogen bond donating capacity and a significant weakening of the capacity to accept a hydrogen bond, which has consequences for molecular recognition. Nevertheless, epitopes based on fluorinated carbohydrate are structurally similar to natural epitopes. Deoxyfluorinated carbohydrates thus can serve as probes in studies focused on protein–carbohydrate interactions in which 19F NMR spectroscopy plays a prominent role [9,10,11]. On the other hand, deoxyfluorination can favorably influence the pharmacological properties of carbohydrate-based drugs, such as potency, lipophilicity, metabolic stability and resistance to the hydrolysis of the glycosidic bond [12,13,14,15,16,17,18,19,20].
The effect of deoxyfluorination on intermolecular interactions has been studied predominantly in solution, while similar studies in the solid state remain scarce [21,22]. In this paper, we focus on intermolecular interactions in the crystal structures of C4-deoxyfluorinated 1,6-anhydro-2,3-epimino-hexopyranoses and their comparison to the parent and deoxygenated molecules. These compounds contain an annulated aziridine (or epimine) ring, a three-membered saturated nitrogen heterocycle. The strained three-membered ring exhibits rich chemical reactivity; aziridine-containing compounds are therefore versatile synthetic intermediates and building blocks amenable to a variety of transformations [23,24,25]. Moreover, aziridine-containing compounds exhibit biological activity, mainly antitumoral [26]; some of them also occur naturally [27]. In the field of carbohydrate chemistry, pyranosides and furanosides with annulated aziridine rings have served mainly as intermediates in the synthesis of amino sugar analogues, the typical transformation being the nucleophilic opening of the three-membered ring [28,29]. In this respect, they function as nitrogen analogues of sugar epoxides. In this study of intermolecular interactions, the β-d-hexopyranose framework is rigidified by the 1,6-anhydro bridge and a 2,3-epimine functionality, generating a rigid tricyclic skeleton. The fluorine substitution takes place at C4 in the vicinity of the aziridine ring [30]. The effect of fluorine substitution on intermolecular interactions in crystal packing is evaluated through a comparison with the structures of the corresponding hydroxyl [31] and dihydrogen [32] analogues at the same C4 position, 10 crystal structures in total.

2. Experiments and Methods

2.1. Synthesis

The synthesis of C4-deoxyfluorinated epimines II, III, VII and VIII and C4-deoxy-epimines I and VI exploited aziridine ring closure through the reduction of vicinal trans azido sulfonates, as described previously [30,32]. The synthesis of gulo-epimine V relies on the aza-Payne rearrangement of vicinal trans azido epoxide [31]. Allo-epimine IV was obtained by cyclization under Mitsunobu conditions from a suitable 2-benzylamino derivative followed by hydrogenolytic N,O-debenzylation [33]. Manno-epimine IX was prepared through the reduction of a vicinal trans azido tosylate31 and hydrogenolytic O-debenzylation [34]. The synthesis of X is described in the Supplementary Materials.

2.2. DFT Calculation Details

For the calculation of the energies of the isolated molecules and their electrostatic potentials, the structures were optimized using the Gaussian09 [35] program, with B3LYP [36,37] as the functional and 6-311G(d,p)++ as a basis set. The vibrational analysis showed that all the structures correspond to the local minima in the potential energy surface. Avogadro software was used for the graphical presentation [38]. The energies of the respective pairs of molecules were obtained in the following manner. First, the coordinates of the selected pair of molecules were obtained from their X-ray structure and the hydrogens were allowed to relax using B3LYP/6-311G(d,p)++, while the coordinates of heavier atoms remained fixed. The optimized pair was used as a starting geometry for energy calculation, using B3LYP/6-311G(2df,2pd)++ and a D3 version of Grimme dispersion with Becke-Johnson damping [39], added with the keyword, empirical dispersion = gd3bj. The energies of all the pairs were then referenced to the pair with the lowest energy; the differences are shown in the following tables. Upon the reviewer’s request, the basis set superposition error (BSSE) [40] was analyzed using pairs of structure III. The obtained energy differences were found to be slightly lower; however, all the trends remained similar (for comparison, see Supplementary Materials). A detailed insight into the nature of the intermolecular interactions involved between molecules can be obtained through energy decomposition analysis (EDA) [41]; however, such an analysis was beyond the scope of this manuscript.

2.3. X-ray Crystallography

Single crystals suitable for X-ray crystallography were obtained from diethyl ether solution. In general, the compounds studied provided colorless crystalline material with a melting point within the range of 90–110 °C [30,31,32].
The diffraction data were collected on a Bruker D8 VENTURE Kappa Duo PHOTON 100 CMOS with monochromated Mo/Cu-Kα radiation. The structures were solved by direct methods (SHELXT [42]) and refined by full-matrix least-squares on F2 values (CRYSTALS [43]). All the heavy atoms were refined anisotropically. The hydrogen atoms were usually localized from the expected geometry and the difference electron density maps and were refined isotropically. ORTEP-3 [44] and a BIOVIA Discovery Studio Visualizer (Accelrys Inc., San Diego, CA, USA [45]) were used for the structure presentation.
The crystallographic data for the structures reported in this paper have been deposited with the Cambridge Crystallographic Data Centre as a supplementary publication. Copies of the data can be obtained free of charge on application to CCDC from the following e-mail address: [email protected].
X-ray of 1,6-anhydro-2,3,4-trideoxy-2,3-epimino-β-d-ribo-hexopyranose I: C6H9NO2, M = 127.14 g/mol, orthorhombic system, space group P21212, a = 9.3443(3), b = 7.9144(3), c = 8.0972(3) Å, Z = 4, V = 598.82(4) Å3, Dc = 1.41 g.cm−3, μ(Mo Kα) = 0.11 mm−1, T = 150 K, crystal dimensions of 0.28 × 0.42 × 0.55 mm. The final model converged to the final R = 0.0300 and Rw = 0.0740 using 1332 independent reflections (θmax = 27.48°). CCDC registration number 2127197.
X-ray of 1,6-anhydro-2,3-dideoxy-2,3-epimino-β-d-allopyranose IV: C6H9NO2, M = 143.14 g/mol, orthorhombic system, space group P212121, a = 7.4175(2), b = 9.1635(2), c = 9.3760(2) Å, Z = 4, V = 637.29(3) Å3, Dc = 1.49 g.cm−3, μ(Mo Kα) = 0.12 mm−1, T = 150 K, crystal dimensions of 0.32 × 0.41 × 0.49 mm. The final model converged to the final R = 0.0256 and Rw = 0.0670 using 4762 independent reflections (θmax = 29.99°). CCDC registration number 2127196.
X-ray of 1,6-anhydro-2,3-dideoxy-2,3-epimino-β-d-gulopyranose V: C6H9NO2, M = 143.14 g/mol, monoclinic system, space group P21, a = 6.0539(10), b = 8.4214(16), c = 6.2298(12) Å, Z = 2, V = 306.43(10) Å3, Dc = 1.55 g.cm−3, μ(Cu Kα) = 1.06 mm−1, T = 150 K, crystal dimensions of 0.03 × 0.08 × 0.29 mm. Due to poor data, the hydrogen atoms were linked to the heavy atoms and were not refined. The final model converged to the final R = 0.0901 and Rw = 0.0240 using 773 independent reflections (θmax = 69.38°). CCDC registration number 2127193.
X-ray of 1,6-anhydro-2,3,4-trideoxy-2,3-epimino-β-d-lyxo-hexopyranose VI: C6H9NO2, M = 127.14 g/mol, orthorhombic system, space group P212121, a = 6.5294(2), b = 7.5004(2), c = 23.9471(5) Å, Z = 8, V = 1172.76(5) Å3, Dc = 1.44 g.cm−3, μ(Mo Kα) = 0.11 mm−1, T = 150 K, crystal dimensions of 0.17 × 0.27 × 0.43 mm. The final model converged to the final R = 0.0296 and Rw = 0.0709 using 2567 independent reflections (θmax = 27.38°). CCDC registration number 2127194.
X-ray of 1,6-anhydro-2,3-dideoxy-2,3-epimino-β-d-mannopyranose IX: C6H9NO3, M = 143.14 g/mol, orthorhombic system, space group P212121, a = 8.6099(2), b = 16.7111(5), c = 17.8865(5) Å, Z = 16, V = 2573.53(12) Å3, Dc = 1.48 g.cm−3, μ(Mo Kα) = 0.12 mm−1, T = 150 K, crystal dimensions of 0.52 × 0.63 × 0.70 mm. The final model converged to the final R = 0.0370 and Rw = 0.0877 using 5523 independent reflections (θmax = 27.53°). CCDC registration number 2127198.
X-ray of 1,6-anhydro-2,3-dideoxy-2,3-epimino-β-d-talopyranose X: C6H9NO2, M = 143.14 g/mol, orthorhombic system, space group P212121, a = 7.4182(2), b = 8.9517(2), c = 9.5837(3) Å, Z = 4, V = 636.410(2) Å3, Dc = 1.49 g.cm−3, μ(Mo Kα) = 0.12 mm−1, T = 150 K, crystal dimensions of 0.17 × 0.33 × 0.50 mm. The final model converged to the final R = 0.0285 and Rw = 0.0694 using 1406 independent reflections (θmax = 27.52°). CCDC registration number 2127195.

3. Results and Discussion

3.1. Nomenclature

The six-membered pyranose ring of monosaccharides is relatively flexible, allowing the molecule to adopt various conformations. The most stable conformations are chair (C), boat (B) and twist (T), which can mutually interchange through less stable conformations such as half-chair (H), screw-boat (S) and envelope (E). The conformation can be fixed through derivatization or the attachment of an additional ring. In the case of this study, it is formal dehydration that bridges carbon atoms C1 and C6 (forming a five-membered 1,3-dioxolane ring), and the annulation of an aziridine (or epimine) ring at C2 and C3. This transformation fixes the six-membered tetrahydropyran ring in an envelope conformation with the pyranose oxygen distorted from the plane defined by carbons C1–C5 [30]. In this molecule, all the carbon atoms are used to fix the conformation except for carbon C4, which can be used for further modification and the subsequent study of the resulting substitution effect. In this paper, the 1,6-anhydrohexopyranoses containing an annulated 2,3-aziridine ring IV, V, IX, and X (Scheme 1) were modified at C4 by deoxygenation (giving rise to compounds I and VI) or deoxyfluorination (leading to compounds II, III, VII and VIII). A combination of an exo- and endo-epimine arrangements [46] with respect to the bicyclic 6,8-dioxabicyclo-[3,2,1]-octane skeleton, together with a substitution to an axial (ax) or equatorial (eq) position at C4, afforded a series of ten structures; five exo- and five endo- 2,3-epimino derivatives of 1,6-anhydro-β-d-hexopyranoses (Scheme 1). The designations cis (compounds II, IV, VIII and X) and trans (compounds III, V, VII and IX) refer to the relationship between the aziridine ring and the C4 substituent. Compounds I, IV, V, VI, IX and X were prepared according to known procedures (see Experimental Section). Single crystals suitable for X-ray crystallography were obtained by crystallization from diethyl ether. The four crystal structures containing C4-fluorinated epimines II, III, VII and VIII were published previously [30] (CSD refcodes GUZPAT, GUZNUL, GUZPEX and GUZPIB, respectively). The crystal structures served as a base for study of the substitution effect on the molecular arrangement in the crystal lattice.

3.2. Options for the Formation of Intermolecular Interactions

Generally, the molecules in the series can utilize two basic types of intermolecular interaction: hydrogen bonding and van der Waals, or weaker electrostatic interactions. Hydrogen bonding utilizes both oxygen atoms (pyranose oxygen O1 and 1,6-anhydro oxygen O2, see Scheme 1 or Figure 1 for numbering) as hydrogen bond acceptors. As these atoms are tightly fixed in the rigid tricyclic skeleton, their electron lone pairs point in the same directions in all the molecules studied. Another atom functioning as an acceptor of hydrogen bond is the nitrogen of the epimine group. The nitrogen lone pair can point inside or outside of the molecule as nitrogen can easily undergo a pyramidal inversion and switch the orientation of its lone pair with hydrogen. The DFT calculations indicated that in most cases, the orientation of the hydrogen inside the molecule was more favorable due to the stabilization via intramolecular N-H···O1/O2 hydrogen bond. The energy difference was much higher when the fluorine or hydroxy groups were in the cis arrangement to the epimine group. In general, the energy difference was significantly higher for the exo-epimines than for the endo-epimines (Table 1).
The intramolecular NH···O1 hydrogen bond was found in three cases of exo-epimine crystal structures (II, IV and V); two of them possessed a cis arrangement of the fluorine or hydroxy group to the epimine (II and IV). The hydrogen atom of the epimine was involved in the intramolecular hydrogen bonding, mainly towards pyranose oxygen O1 (Figure 1). In these structures, the hydrogen atom was disqualified from participation in intermolecular interactions in the molecular packing. The epimine group could then operate only as an acceptor of hydrogen bond via nitrogen lone pair. In the rest of the exo-epimine structures (I and III), NH was found as an important donor of hydrogen bond. The formation of the intramolecular hydrogen bond of the epimine group in endo-epimines was also observed in solution by IR spectroscopy. However, in all the solid-state structures presented here, the lone nitrogen electron pair of endo-epimines was oriented towards C6, where it was involved in a weak interaction with the endo C6 hydrogen (Figure 1).
The deoxyfluorination at C4 introduced fluorine as another hydrogen bond acceptor with non-specific directionality, while the C4 hydroxy group could act as a simultaneous donor and acceptor of hydrogen bonding. Contrary to the fixed orientation of the NH group, the OH group could freely rotate around the C4-O bond and adopt any orientation, depending on which was the most favorable for the formation of a hydrogen bond. The structures containing two hydrogen atoms at C4 could utilize these atoms only in weak electrostatic interactions as they were located in the electro-positively charged part of the molecule. The electro-positive region passed through all hydrogen atoms located on the carbon skeleton. Apart from C6, all the carbon atoms were located in the equatorial part of the molecule and created a strip from C1 to C5, where it was interrupted by O1 (Figure 2). By contrast, the electro-negatively charged atoms were located on a vertical axis, represented mainly by O1 and O2. The location of the formed electronegative strip depended on the orientation of the epimine group; it was partially located at the lower or upper part of the molecule for the exo-epimines and endo-epimines, respectively. Further extension of the electronegative region was provided by fluorine and hydroxy substituents, depending on their particular placement.

3.3. The Strongest Intermolecular Interactions Found in I–X

The above-mentioned types of intermolecular interaction were found in molecules surrounding the parent molecule in the crystal lattice. In general, the number of surrounding molecules varied depending on the size, shape and chemical nature of the molecule. In the structures of the epimines studied, the number varied from 12 to 14, with six molecules forming an equatorial ring surrounding the parent molecule from C1 to O1 and three or four molecules above and below approaching the molecule from the top (C6 and O2) and bottom, respectively (Figure 3). The surrounding molecules were generated from the parent molecule by a symmetry operation or translation.
The strength of individual interactions was estimated via DFT calculations, where the energy of each pair of interacting molecules was calculated and mutually compared within a single crystal structure. This analysis identified the strongest interaction and provided an overall energy range of interacting pairs in a given crystal lattice. The complete list of DFT calculation results including the numbering of molecular pairs in each structure can be found in the Supplementary Materials. The intermolecular interaction of NH with O1 serving as an acceptor of hydrogen bond was found in almost all C4 deoxygenated and C4 deoxyfluorinated epimine derivatives. In these structures, the energy difference between the strongest and weakest interaction in given crystal lattices ranged from 4.20 to 5.07 kcal/mol. The only exception was II, which is discussed below in detail. In the structures of the hydroxy epimines, interactions involving the hydroxy group were found to be the strongest. The nitrogen atom usually acted as a hydrogen bond acceptor via the interaction of its lone pair. The energy difference ranged in hydroxy epimines from 8.20 to 9.11 kcal/mol, indicating the formation of much stronger intermolecular interactions than in the rest of the structures. The smallest energy difference between molecular interactions was found in the structure of exo-epimine II, where NH was involved in intramolecular interaction with fluorine and pyranose oxygen. The strongest interaction found in the molecular packing of II was the interaction between the electron-poor and -rich regions of two adjacent molecules, which was followed by other weak interactions also involving the fluorine atom. The strongest interactions found in the structures of the exo- and endo-epimines can be seen in Figure 4 and Figure 5, respectively.
In general, the fluorine atom did not act as an acceptor of hydrogen bonds donated by the OH or NH group in the structures studied. The fluorine atom was mainly involved in weaker interactions with hydrogen atoms localized on the carbon skeleton. The interaction of fluorine in combination with other weak interactions could lead to rather strong associations with the parent molecule. In II, the fluorine interaction with hydrogen at C2 of the epimine was supported by the interaction of C2 and C6 hydrogen with nitrogen and pyranose oxygen (ΔE = 1.16 kcal/mol, molecule no. 9). In VII, fluorine interacted with C4 hydrogen of molecule no. 9, which interacted reversely by its fluorine with C3 hydrogen. This double fluorine interaction of two molecules featured ΔE = 1.60 kcal/mol. In VIII, fluorine interaction with C5 hydrogen was supported by the interaction of C4 with the nitrogen lone pair (ΔE = 0.90 kcal/mol, molecule no. 10). In III, the fluorine interaction was not specific. The closest hydrogen was located at C2 of molecule no. 5 without any other support, resulting in one of the weakest interactions in the structure (ΔE = 4.95 kcal/mol). However, it is worth mentioning that the interaction of the fluorine atom was not restricted to one molecule but interacted weakly with two or three other molecules in the surroundings and contributed significantly to the molecular arrangement in the surroundings of the central molecule.

3.4. Comparison of Molecular Arrangements in Structures VI and IX Containing More Independent Molecules

The substitution effect on the formation of intermolecular interactions was inspected through the mutual comparison of molecular packing in the epimine structures. The role of the epimine group was assessed through a separated comparison of exo and endo structures; similar motifs in crystal packing should be found among structures with the same substitution or, alternatively, some features that are independent of the substitution could also be revealed across all the structures. In addition to the mutual comparison of all the structures, the structures containing more molecules in the independent part of the unit cell provided unique material for the detailed assessment of the capabilities of a given molecule for the formation of intermolecular interactions. In particular, the structure of C4 deoxygenated endo-epimine VI contained two independent molecules, denoted as VIa and VIb, while the structure of C4-OHax endo-epimine IX contained four molecules (IXa–d). It is very likely that each independent molecule would somehow utilize different intermolecular interactions despite possessing the same or similar conformation. In the case of VI, the independent molecules VIa and VIb were each surrounded by six molecules of VIa and six molecules of VIb, but no obvious similarities were apparent in the molecular arrangement around the parent molecule. Both molecules formed the strongest interaction via the hydrogen bonding of their NH group. While in VIa, NH interacted with nitrogen lone pair of an adjacent molecule, in VIb, NH interacted with a lone pair of pyranose oxygen. The latter interaction was 0.4 kcal/mol weaker. The rest of the intermolecular interactions were provided by weak electrostatic interactions. However, a combination of two such weak interactions could lead to the formation of a quite strong interaction between two molecules with an energy difference of around 1 kcal/mol when compared to the strongest interaction found in the structure. An overview of the strongest intermolecular interactions found in VI can be seen in Figure 6.
As mentioned above, the independent part of the unit cell in the structure of IX was formed by four molecules. The geometries of all four molecules were practically identical, including the orientation of the hydroxy group, which differed among molecules IXa–d by only 16° (Figure 7a). Each independent molecule was surrounded by 12 molecules; for IXa and also for IXd it was 2(IXa)-4(IXb)-4(IXc)-2(IXd), for IXb 4-0-4-4, and 4-4-0-4 for IXc (see Supplementary Materials). The strongest interactions found in the structure involved the hydroxy group. In the case of IXa–c, the nitrogen lone pair served as an acceptor of hydrogen bond from the OH group, while in IXd, OH from IXc was the acceptor. The NH group as a donor of hydrogen bonds provided slightly weaker interactions. The acceptors of this interaction were NH in IXa, OH in IXb and O1 in IXc,d (Table 2). An interesting feature of IX was the mutual position of OH and NH groups in the molecule. It seems that both groups could be interchanged (by rotation of the molecule), while the ability of the molecule to participate in weaker interactions remained almost unaffected as the original electro-negative/positive regions were replaced by electro-negative/positive regions from the opposite side of the molecule (Figure 7b). This feature was apparent when the molecular packing around IXa was compared to that of IXb. The only difference was in the acceptor of the hydrogen bond from the NH group (Table 2), which was caused precisely by the exchange of OH and NH in intermolecular interactions. Otherwise, the molecules surrounding the parent molecules IXa and IXb were in similar positions, utilizing similar interactions (Figure 8a). Similarly to IXa and IXb, the hydrogen bonds donated by the OH group were also accepted in IXc by the nitrogen lone pair of another molecule. On the other hand, this was the only interaction common to all three molecules; the rest of the interactions were of a different origin in IXc. The molecules surrounding IXc were in similar positions, as in IXa and IXb, but a similar packing motif was found only for molecules tied by OH···NH interactions. As a parent molecule, IXd accepted hydrogen bonds from OH by its NH group, similarly to IXa and IXb. However, the greatest number of similarities in molecular packing was found for IXd and IXc. Furthermore, in this case, the OH group was exchanged in some interactions by NH, while the arrangement of the other molecules remained the same (Figure 8b).

3.5. Comparison of Molecular Arrangements in Exo-Epimines I–V and in Endo-Epimines VI–X

The comparison of packing motifs within the group of exo- or endo-epimines should reflect the role of common molecular fragments i.e., the aziridine group, the carbon skeleton, bridging oxygen atoms. The question is whether the effect of these fragments is preserved in the presence of a different substitution.
In the crystal structures of exo-epimines I–V, the intramolecular hydrogen bonding of the epimine moiety was preserved in three of them. This fact, in combination with varying substitution at C4, resulted in different molecular packing; a thorough analysis did not show any common intermolecular interaction. The only similarity was found in weak interactions in structure II and V, where hydrogen atoms at C6 approached the pyranose oxygen O1 and hydrogen atoms at C5 approached the anhydro oxygen O2. In both structures, two molecules in the surroundings of the parent molecule served alternately as donors and acceptors of these weak interactions. Both molecules were coupled with the parent molecule by the same symmetry operation (Figure 9). The same nature of interactions binding both molecules to the parent molecule was documented by the same energy difference calculated by DFT (see Supplementary Materials).
Contrary to the exo-epimines, the endo-epimines VI–X did not form an intramolecular hydrogen bond; thus, the configuration of the nitrogen atom was the same in all the structures studied in this group. The analysis of the intermolecular interactions in five endo-epimine structures was based on a comparison of nine molecular packings as a consequence of the presence of two and four independent molecules in the crystals of VI and IX, respectively, as discussed above. The intermolecular hydrogen bonding of NH towards pyranose oxygen O1 was found in all the structures. This interaction was the strongest in the unsubstituted and fluorine-substituted derivatives, while in hydroxy derivatives it played only a supportive role and the strongest interaction involved primarily the OH group. Surprisingly, no common motif was found in the structures based on NH···O1 interactions as the molecules involved possessed different mutual orientations (Figure 10).
Different arrangements of adjacent molecules utilizing NH hydrogen bonding together with the interactions of different substituents led to different molecular assembly in these structures. Nevertheless, certain similarities were found in the crystal packing of some endo-epimines; these can be attributed to weaker interactions. A detailed discussion of the similar packing motifs found in endo-epimines can be found in the Supplementary Materials.

3.6. Comparison of Molecular Arrangements in the Ax- and Eq-Substituted Epimines

A comparison of the structures with identical substituents in the same orientation, axial or equatorial, can reveal whether the substituent determines the arrangement of the molecules in the crystal lattice regardless of the orientation of the aziridine group.
In the structures of the deoxygenated epimines I and VI, namely VIa, the molecules in the surroundings of the parent molecule were in practically the same position, although the strongest interaction was slightly different in both structures. In both cases, the strongest interaction involved the NH group as a donor of the hydrogen bond. While in I, the acceptor of this hydrogen bond was pyranose oxygen O1, in VIa, it was a nitrogen lone pair. Interestingly, both molecules, which employed strong interactions, were in the same spatial position in both structures, differing only in orientation. This feature enabled the placing of the rest of the molecules in the same position, as well as applying similar interactions to the parent molecule. A certain portion of the molecules utilized exactly the same interactions; these molecules also possessed a similar orientation towards the parent molecule in both structures. The other molecules were in different orientations, which was convenient for the formation of intermolecular interactions (Figure 11). The mutual rotation of the molecules in the same positions compensated to a certain degree for the different orientation of the NH group in the exo- and endo-epimines. It is worth noting that when rotated by 180°, a single molecule of I possesses a similar distribution of electrostatic potential on the surface as found in molecule VI merely by replacing NH group by CH2. This feature probably contributed significantly to the similarity found in both molecular arrangements. On the other hand, no similarities were found for I and VIb. Obviously, the orientation of NH did not determine the molecular arrangement around VIa, while for VIb it did.
The association of the molecules in the structures of C4-axial fluoro epimines II and VII appeared different at first sight as only the molecules on the side of the parent molecule seemed to be at least in similar positions. Nevertheless, both structures utilized similar intermolecular interactions involving fluorine atoms. In both structures, the mutual interaction of the substituents at C4 gave rise to molecular chains based primarily on the interaction between hydrogen at C3 and C5 and the fluorine atom of the adjacent molecule. In a simplified way, the interactions within one chain can be described as ···HCCF···HCCF···HCCF··· (Figure 12). When the structures were aligned in the chain direction, all the molecules fitted almost perfectly to their counterparts in the other structure, except for the parent molecules, which were then mutually rotated by approximately 28° around the horizontal axis (Figure 13). The identical molecular assembly in both structures manifested the effect of fluorine atom, which did not form a strong interaction with a single molecule but interacted weakly with at least three other molecules in the proximate surroundings. This feature determined the molecular arrangement in the whole structure, even though there were stronger interactions present.
In the structures of C4-equatorial fluoro epimines III and VIII, a similar arrangement was found only for two molecules approaching the parent molecule from the side. Interacting molecules utilized similar interactions of fluorine atoms with hydrogen at C2 (in VIII, it is supported by interaction with hydrogen at C1). These molecules were generated by translation of the parent molecule and the fluorine interaction was not supported by any other interaction. This arrangement was tied together by the weakest interaction in both structures (molecules nos. 2 and 5, see Figure 14 and Supplementary Materials). However, the same orientation of the fluorine yielded a similar molecule arrangement in its vicinity in both structures, despite the formal weakness of the interaction, as suggested by the DFT calculations.
A comparison of the unmodified carbohydrates with the same orientation of the C4 hydroxy group, axial IV and IX, and equatorial V and X did not reveal any common features in their molecular arrangements. In these four structures, hydrogen bonding of OH as the strongest interaction played a dominant role in the molecule arrangement. The involvement of NH in the intramolecular hydrogen bond in both exo-epimine structures IV and V was probably the main reason for the completely different packing of both groups. As discussed above, in IV and V, the NH group served only as an acceptor of intermolecular hydrogen bonds, while in IX and X it served as both donor and acceptor. The most favorable molecule arrangement found in the crystal lattice of C4-OHax epimines IV and IX directed the O-H bond to different directions (Figure 15a). The C4-OHeq epimines V and X utilized OH···NH in the same direction; however, the interacting molecules approached the parent molecule from a different direction (Figure 15b), which can also be seen as a consequence of the different orientation of the NH group.

4. Conclusions

The effect of substitution on intermolecular interactions was investigated in a series of 1,6-anhydro-2,3-epimino-hexopyranoses. The strength of intermolecular interactions was assessed using DFT calculations, providing the background for a detailed comparison of the molecular arrangements in ten crystal structures, which included two structures of C4-deoxygenated, four C4-deoxyfluorinated and four parent carbohydrates. It was found that the substitution of the original hydroxy group by hydrogen or fluorine led to a narrowing of the range between the strongest and the weakest intermolecular interactions in a given structure by approximately 4 kcal/mol. The strongest interactions were found for the hydrogen bonding of hydroxy groups, followed by the hydrogen bonding of the aziridine amino group, interactions with fluorine and weak electrostatic interactions, in descending order. The intermolecular interactions that involved the fluorine atom were rather weak; however, in the structures studied they were often strengthened by other weak interactions. The fluorine atom was not able to substitute the role of the hydroxy group in the molecular packing in any of the structures. The fluorine atoms interacted solely with the hydrogen atoms located at electropositive regions of the carbohydrate molecules. A comparison of the molecular arrangements in the exo- and endo-epimines did not show a common repetitive motif, except for weak interactions below and above the parent molecule, which involved interactions of electropositive regions with pyranose and anhydro oxygen atoms. These interactions were found to be among the weakest in the structures studied, and they definitely did not determine the overall arrangement around the parent molecule. A comparison of the corresponding exo- and endo-epimines with the same C4 substituent and orientation (axial or equatorial) showed that the orientation of the aziridine group did not determine the arrangement of the molecules in the crystal lattice. Similar features were found for both the deoxygenated and the deoxyfluorinated epimines regardless of the orientation of the aziridine group. The fluorine atoms in particular were found to utilize identical interactions in the groups of C4-Fax and C4-Feq epimines. As a consequence, the molecular arrangement was found to be identical in both the C4-Fax epimines, while in the C4-Feq epimines, only the molecules near the fluorine were found in similar positions. This feature reflects the fact that interactions of single fluorine atoms are not restricted to a single molecule but are spread over at least three other molecules in the surroundings. This eventually leads to similar molecular arrangements in the structures of related compounds.
The conclusions derived from the series of 1,6-anhydro-2,3-epimino-hexopyranoses indicate the role of fluorine atoms in molecular arrangements in the crystal lattice and can help in the design of functional molecules for crystal engineering and related fields.

Supplementary Materials

The following supporting information can be downloaded. Page S2: synthesis of compound X. Tables SI–SX: calculated energy of interacting molecular pair., Pages S9–12: comparison of molecular arrangement in endo-epimines VI–X.

Author Contributions

Conceptualization, J.S. and J.K.; investigation, J.K., M.J and I.C.; writing—original draft preparation, J.S.; writing—review and editing, J.S, J.K. and M.J.; visualization, J.S. All authors have read and agreed to the published version of the manuscript.

Funding

Computational resources were supplied by the project “e-Infrastruktura CZ” (e-INFRA CZ LM2018140) supported by the Ministry of Education, Youth and Sports of the Czech Republic.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data available upon request.

Acknowledgments

The authors are grateful to Andrew Christensen (Boston University) for proofreading.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Tamburrini, A.; Colombo, C.; Bernardi, A. Design and synthesis of glycomimetics: Recent advances. Med. Res. Rev. 2020, 40, 495–531. [Google Scholar] [CrossRef]
  2. Uhrig, M.L.; Lantaño, B.; Postigo, A. Synthetic strategies for fluorination of carbohydrates. Org. Biomol. Chem. 2019, 17, 5173–5189. [Google Scholar] [CrossRef] [PubMed]
  3. Council, C.E.; Kilpin, K.J.; Gusthart, J.S.; Allman, S.A.; Linclau, B.; Lee, S.S. Enzymatic glycosylation involving fluorinated carbohydrates. Org. Biomol. Chem. 2020, 18, 3423–3451. [Google Scholar] [CrossRef] [PubMed]
  4. Bilska-Markowska, M.; Szwajca, A.; Marciniak, B. Design, properties and applications of fluorinated and fluoroalkylated N-containing monosaccharides and their analogues. J. Fluorine Chem. 2019, 227, 109364. [Google Scholar] [CrossRef]
  5. Böhm, H.-J.; Banner, D.; Bendels, S.; Kansy, M.; Kuhn, B.; Müller, K.; Obst-Sander, U.; Stahl, M. Fluorine in Medicinal Chemistry. ChemBioChem 2004, 5, 637–643. [Google Scholar] [CrossRef] [PubMed]
  6. O’Hagan, D. Understanding organofluorine chemistry. An introduction to the C–F bond. Chem. Soc. Rev. 2008, 37, 308–319. [Google Scholar] [CrossRef] [PubMed]
  7. Hagmann, W.K. The Many Roles for Fluorine in Medicinal Chemistry. J. Med. Chem. 2008, 51, 4359–4369. [Google Scholar] [CrossRef] [PubMed]
  8. Linclau, B.; Arda, A.; Reichardt, N.C.; Sollogoub, M.; Unione, L.; Vincent, S.P.; Jimenez-Barbero, J. Fluorinated carbohydrates as chemical probes for molecular recognition studies. Current status and perspectives. Chem. Soc. Rev. 2020, 49, 3863–3888. [Google Scholar] [CrossRef] [PubMed]
  9. Diercks, T.; Ribeiro, J.P.; Cañada, F.J.; André, S.; Jiménez-Barbero, J.; Gabius, H.-J. Fluorinated Carbohydrates as Lectin Ligands: Versatile Sensors in 19F-Detected Saturation Transfer Difference NMR Spectroscopy. Chem. Eur. J. 2009, 15, 5666–5668. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  10. Ribeiro, J.P.; Diercks, T.; Jiménez-Barbero, J.; André, S.; Gabius, H.-J.; Cañada, F.J. Fluorinated Carbohydrates as Lectin Ligands: 19F-Based Direct STD Monitoring for Detection of Anomeric Selectivity. Biomolecules 2015, 5, 3177–3192. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  11. Kurfiřt, M.; Dračínský, M.; Červenková Šťastná, L.; Cuřínová, P.; Hamala, V.; Hovorková, M.; Bojarová, P.; Karban, J. Selectively Deoxyfluorinated N-Acetyllactosamine Analogues as 19F NMR Probes to Study Carbohydrate-Galectin Interactions. Chem. Eur. J. 2021, 27, 13040–13051. [Google Scholar] [CrossRef]
  12. O’Hagan, D.; Young, R.J. Accurate Lipophilicity (log P) Measurements Inform on Subtle Stereoelectronic Effects in Fluorine Chemistry. Angew. Chem. Int. Ed. 2016, 55, 3858–3860. [Google Scholar] [CrossRef]
  13. Denavit, V.; Lainé, D.; Bouzriba, C.; Shanina, E.; Gillon, É.; Fortin, S.; Rademacher, C.; Imberty, A.; Giguère, D. Stereoselective Synthesis of Fluorinated Galactopyranosides as Potential Molecular Probes for Galactophilic Proteins: Assessment of Monofluorogalactoside–LecA Interactions. Chem. Eur. J. 2019, 25, 4478–4490. [Google Scholar] [CrossRef]
  14. St-Gelais, J.; Cote, E.; Laine, D.; Johnson, P.A.; Giguere, D. Addressing the Structural Complexity of Fluorinated Glucose Analogues: Insight into Lipophilicities and Solvation Effects. Chem. Eur. J. 2020, 26, 13499–13506. [Google Scholar] [CrossRef]
  15. Baumann, A.; Marchner, S.; Daum, M.; Hoffmann-Röder, A. Synthesis of Fluorinated Leishmania Cap Trisaccharides for Diagnostic Tool and Vaccine Development. Eur. J. Org. Chem. 2018, 2018, 3803–3815. [Google Scholar] [CrossRef]
  16. Johannes, M.; Reindl, M.; Gerlitzki, B.; Schmitt, E.; Hoffmann-Röder, A. Synthesis and biological evaluation of a novel MUC1 glycopeptide conjugate vaccine candidate comprising a 4’-deoxy-4’-fluoro-Thomsen–Friedenreich epitope. Beilstein J. Org. Chem. 2015, 11, 155–161. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  17. Selnick, H.G.; Hess, J.F.; Tang, C.; Liu, K.; Schachter, J.B.; Ballard, J.E.; Marcus, J.; Klein, D.J.; Wang, X.; Pearson, M.; et al. Discovery of MK-8719, a Potent O-GlcNAcase Inhibitor as a Potential Treatment for Tauopathies. J. Med. Chem. 2019, 62, 10062–10097. [Google Scholar] [CrossRef] [Green Version]
  18. Lee, H.-Y.; Chen, C.-Y.; Tsai, T.-I.; Li, S.-T.; Lin, K.-H.; Cheng, Y.-Y.; Ren, C.-T.; Cheng, T.-J.R.; Wu, C.-Y.; Wong, C.-H. Immunogenicity Study of Globo H Analogues with Modification at the Reducing or Nonreducing End of the Tumor Antigen. J. Am. Chem. Soc. 2014, 136, 16844–16853. [Google Scholar] [CrossRef]
  19. Oberbillig, T.; Mersch, C.; Wagner, S.; Hoffmann-Röder, A. Antibody recognition of fluorinated MUC1 glycopeptide antigens. Chem. Commun. 2012, 48, 1487–1489. [Google Scholar] [CrossRef] [PubMed]
  20. Garnett, J.A.; Liu, Y.; Leon, E.; Allman, S.A.; Friedrich, N.; Saouros, S.; Curry, S.; Soldati-Favre, D.; Davis, B.G.; Feizi, T.; et al. Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii. Protein Sci. 2009, 18, 1935–1947. [Google Scholar] [CrossRef]
  21. Mehta, G.; Sen, S. Probing Fluorine Interactions in a Polyhydroxylated Environment: Conservation of a C–F···H–C Recognition Motif in Presence of O–H···O Hydrogen Bonds. Eur. J. Org. Chem. 2010, 2010, 3387–3394. [Google Scholar] [CrossRef]
  22. Müller, K. Simple Vector Considerations to Assess the Polarity of Partially Fluorinated Alkyl and Alkoxy Groups. CHIMIA Int. J. Chem. 2014, 68, 356–362. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Yudin, A.K. Aziridines and Epoxides in Organic Synthesis; John Wiley and Sons: Weinheim, Germany, 2006; pp. 1–492. [Google Scholar]
  24. Sweeney, J.B. Aziridines: Epoxides’ ugly cousins? Chem. Soc. Rev. 2002, 31, 247–258. [Google Scholar] [CrossRef] [PubMed]
  25. Stanković, S.; D’Hooghe, M.; Catak, S.; Eum, H.; Waroquier, M.; Van Speybroeck, V.; De Kimpe, N.; Ha, H.-J. Regioselectivity in the ring opening of non-activated aziridines. Chem. Soc. Rev. 2012, 41, 643–665. [Google Scholar] [CrossRef]
  26. Degennaro, L.; Trinchera, P.; Luisi, R. Recent Advances in the Stereoselective Synthesis of Aziridines. Chem. Rev. 2014, 114, 7881–7929. [Google Scholar] [CrossRef] [PubMed]
  27. Botuha, C.; Chemla, F.; Ferreira, F.; Pérez-Luna, A. Aziridines in Natural Product Synthesis. In Heterocycles in Natural Product Synthesis; John Wiley and Sons: Weinheim, Germany, 2011; pp. 1–39. [Google Scholar]
  28. Karban, J.; Kroutil, J. Chemistry of carbohydrate aziridines. In Advances in Carbohydrate Chemistry and Biochemistry; Horton, D., Ed.; Elsevier Academic Press Inc.: San Diego, CA, USA, 2006; Volume 60, pp. 27–101. [Google Scholar]
  29. Karban, J.; Kroutil, J.; Budesinsky, M.; Sykora, J.; Cisarova, I. Ring-Opening Reactions of Aziridines Fused to a Conformationally Locked Tetrahydropyran Ring. Eur. J. Org. Chem. 2009, 36, 6399–6406. [Google Scholar] [CrossRef]
  30. Karban, J.; Sýkora, J.; Kroutil, J.; Císařová, I.; Padělková, Z.; Buděšínský, M. Synthesis of All Configurational Isomers of 1,6-Anhydro-2,3,4-trideoxy-2,3-epimino-4-fluoro-β-d-hexopyranoses. J. Org. Chem. 2010, 75, 3443–3446. [Google Scholar] [CrossRef]
  31. Karban, J.; Budesinsky, M.; Cerny, M.; Trnka, T. Synthesis and NMR spectra of 1,6-anhydro-2,3-dideoxy-2,3-epimino- and 1,6-anhydro-3,4-dideoxy-3,4-epimino-β-d-hexopyranoses. Collect. Czech. Chem. Commun. 2001, 66, 799–819. [Google Scholar] [CrossRef]
  32. Karban, J.; Budesinsky, M.; Kroutil, J. Synthesis of 1,6-anhydro-2,3,4-trideoxy-2,3-epimino- and 1,6-anhydro-2,3,4-trideoxy-3,4-epimino-β-d-hexopyranoses and their NMR and infrared spectra. Collect. Czech. Chem. Commun. 2004, 69, 1939–1954. [Google Scholar] [CrossRef]
  33. Kroutil, J.; Trnka, T.; Buděšínský, M.; Černý, M. Preparation of 2,3-dideoxy-2,3-epimino and 3,4-dideoxy-3,4-epimino derivatives of 1,6-anhydro-β-d-hexopyranoses by Mitsunobu reaction. Collect. Czech. Chem. Commun. 1998, 63, 813–825. [Google Scholar] [CrossRef]
  34. Cerny, M.; Elbert, T.; Pacak, J. Syntheses with anhydro sugars. 21. Preparation of 1,6-anhydro-2,3-dideoxy-2,3-epimino-β-d-mannopyranose and its conversion to 2-amino-1,6-anhydro-2-deoxy-beta-mannopyranose. Collect. Czech. Chem. Commun. 1974, 39, 1752–1767. [Google Scholar] [CrossRef]
  35. Frisch, M.J.; Trucks, G.W.; Schlegel, H.B.; Scuseria, G.E.; Robb, M.A.; Cheeseman, J.R.; Scalmani, G.; Barone, V.; Mennucci, B.; Petersson, G.A.; et al. Gaussian 09; Revision A.02.; Gaussian, Inc.: Wallingford, CT, USA, 2009. [Google Scholar]
  36. Becke, A.D. Density-functional Thermochemistry. III. The Role of Exact Exchange. J. Chem. Phys. 1993, 98, 5648–5652. [Google Scholar] [CrossRef] [Green Version]
  37. Perdew, J.P.; Wang, Y. Accurate and Simple Analytic Representation of the Electron-Gas Correlation Energy. Phys. Rev. B 1992, 45, 13244–13249. [Google Scholar] [CrossRef] [PubMed]
  38. Avogadro: An Open-Source Molecular Builder and Visualization Tool. Version 1.2.0. 2016. Available online: http://avogadro.cc/ (accessed on 30 August 2021).
  39. Grimme, S.; Ehrlich, S.; Goerigk, L. Effect of the damping function in dispersion corrected density functional theory. J. Comp. Chem. 2011, 32, 1456–1465. [Google Scholar] [CrossRef]
  40. Hobza, P.; Müller-Dethlefs, K. Non-Covalent Interactions: Theory and Experiment; Royal Society of Chemistry: Cambridge, UK, 2010. [Google Scholar]
  41. Stasyuk, O.A.; Sedlak, R.; Guerra, C.F.; Hobza, P. Comparison of the DFT-SAPT and Canonical EDA Schemes for the Energy Decomposition of Various Types of Noncovalent Interactions. J. Chem. Theory Comput. 2018, 14, 3440–3450. [Google Scholar] [CrossRef]
  42. Sheldrick, G.M. SHELXL-2017/1, Program for the Solution of Crystal Structures; University of Göttingen: Göttingen, Germany, 2017. [Google Scholar]
  43. Betteridge, P.W.; Carruthers, J.R.; Cooper, R.I.; Prout, K.; Watkin, D.J.J. CRYSTALS version 12: Software for guided crystal structure analysis. Appl. Cryst. 2003, 36, 1487. [Google Scholar] [CrossRef]
  44. Farrugia, L.J. ORTEP-3 for Windows—A version of ORTEP-III with a Graphical User Interface (GUI). J. Appl. Cryst. 1997, 30, 565. [Google Scholar] [CrossRef]
  45. Discovery Studio Visualizer, Version 17.2.0.16349; BIOVIA, Dassault Systèmes: San Diego, CA, USA, 2016.
  46. Moss, G.P. Basic terminology of stereochemistry (IUPAC Recommendations 1996). Pure Appl. Chem. 1996, 68, 2193–2222. [Google Scholar] [CrossRef]
Molecules 27 00278 sch001 550
Scheme 1. An overview of the series.
Scheme 1. An overview of the series.
Molecules 27 00278 sch001
Molecules 27 00278 g001 550
Figure 1. ORTEP presentation of structure II (left) and VIII (right) showing the intramolecular stabilization of NH.
Figure 1. ORTEP presentation of structure II (left) and VIII (right) showing the intramolecular stabilization of NH.
Molecules 27 00278 g001
Molecules 27 00278 g002 550
Figure 2. An electrostatic potential mapped on the surface of I; electro-positive regions in blue, electro-negative regions in red.
Figure 2. An electrostatic potential mapped on the surface of I; electro-positive regions in blue, electro-negative regions in red.
Molecules 27 00278 g002
Molecules 27 00278 g003 550
Figure 3. Molecular packing in I (left) and coding of the surrounding molecules expressed as molecular centroids (right).
Figure 3. Molecular packing in I (left) and coding of the surrounding molecules expressed as molecular centroids (right).
Molecules 27 00278 g003
Molecules 27 00278 g004 550
Figure 4. ORTEP presentation of the strongest intermolecular interactions found in the structures of exo-epimines I–V. * denotes a parent molecule.
Figure 4. ORTEP presentation of the strongest intermolecular interactions found in the structures of exo-epimines I–V. * denotes a parent molecule.
Molecules 27 00278 g004
Molecules 27 00278 g005 550
Figure 5. ORTEP presentation of the strongest intermolecular interactions found in the structures of endo-epimines VI–X. * denotes a parent molecule.
Figure 5. ORTEP presentation of the strongest intermolecular interactions found in the structures of endo-epimines VI–X. * denotes a parent molecule.
Molecules 27 00278 g005
Molecules 27 00278 g006 550
Figure 6. ORTEP presentation of the strongest intermolecular interactions found in VI. * denotes a parent molecule.
Figure 6. ORTEP presentation of the strongest intermolecular interactions found in VI. * denotes a parent molecule.
Molecules 27 00278 g006
Molecules 27 00278 g007 550
Figure 7. Overlaid independent molecules IX (a); electrostatic potential mapped on the surface of IX (b), top view (left), bottom view (right).
Figure 7. Overlaid independent molecules IX (a); electrostatic potential mapped on the surface of IX (b), top view (left), bottom view (right).
Molecules 27 00278 g007
Molecules 27 00278 g008 550
Figure 8. An overlay of the molecules surrounding IXa (blue) and IXb (red) parent molecule (a), and IXc (orange) and IXd (green) parent molecule (b); molecules in the back (left) and in the front (right). Parent molecules are depicted in gray. Dashed circles indicate the molecules mutually rotated by 180° with the switched OH and NH interaction.
Figure 8. An overlay of the molecules surrounding IXa (blue) and IXb (red) parent molecule (a), and IXc (orange) and IXd (green) parent molecule (b); molecules in the back (left) and in the front (right). Parent molecules are depicted in gray. Dashed circles indicate the molecules mutually rotated by 180° with the switched OH and NH interaction.
Molecules 27 00278 g008
Molecules 27 00278 g009 550
Figure 9. An overlay of similar segments in molecular packing in II (green) and V (red). Parent molecules are depicted in gray.
Figure 9. An overlay of similar segments in molecular packing in II (green) and V (red). Parent molecules are depicted in gray.
Molecules 27 00278 g009
Molecules 27 00278 g010 550
Figure 10. Different orientations of adjacent molecules utilizing hydrogen bonding of NH towards pyranose oxygen O1 in structures VI (gray), VII (green) and IX (red).
Figure 10. Different orientations of adjacent molecules utilizing hydrogen bonding of NH towards pyranose oxygen O1 in structures VI (gray), VII (green) and IX (red).
Molecules 27 00278 g010
Molecules 27 00278 g011 550
Figure 11. An overlay of molecular arrangements around I (red) and VIa (blue), molecules in the back (left) and in the front (right). Parent molecules are depicted in gray (a). Dashed circles indicate different orientation of the molecules switching in interaction CH2 and NH group (black) and positioning NH group to the same location (green). Detail of the different orientation of the molecules (b).
Figure 11. An overlay of molecular arrangements around I (red) and VIa (blue), molecules in the back (left) and in the front (right). Parent molecules are depicted in gray (a). Dashed circles indicate different orientation of the molecules switching in interaction CH2 and NH group (black) and positioning NH group to the same location (green). Detail of the different orientation of the molecules (b).
Molecules 27 00278 g011
Molecules 27 00278 g012 550
Figure 12. Interaction of fluorine atoms in the molecular packing of II (left) and VII (right).
Figure 12. Interaction of fluorine atoms in the molecular packing of II (left) and VII (right).
Molecules 27 00278 g012
Molecules 27 00278 g013 550
Figure 13. Front view (left) of overlaid structures II (green) and VII (red), molecules in the back (left) and in the front (right). Front view (right) of overlaid structures II (green) and VII (red) where VII is rotated approximately by 28°. Parent molecules are depicted in gray.
Figure 13. Front view (left) of overlaid structures II (green) and VII (red), molecules in the back (left) and in the front (right). Front view (right) of overlaid structures II (green) and VII (red) where VII is rotated approximately by 28°. Parent molecules are depicted in gray.
Molecules 27 00278 g013
Molecules 27 00278 g014 550
Figure 14. A chain formation in the structures III (top) and VIII (bottom).
Figure 14. A chain formation in the structures III (top) and VIII (bottom).
Molecules 27 00278 g014
Molecules 27 00278 g015 550
Figure 15. The intermolecular interaction of the OH group in the C4-OHax epimines (a) and the C4-OHeq epimines (b).
Figure 15. The intermolecular interaction of the OH group in the C4-OHax epimines (a) and the C4-OHeq epimines (b).
Molecules 27 00278 g015
Table
Table 1. The difference in energy between identical molecules having NH oriented inside and outside of the molecule provided by DFT calculations.
Table 1. The difference in energy between identical molecules having NH oriented inside and outside of the molecule provided by DFT calculations.
HF-cisF-transOH-cisOH-trans
exo ΔEout-in kcal/mol1.62 (I)3.31 (II)0.83 (III)3.88 (IV)0.86 (V)
endo ΔEout-in kcal/mol0.24 (VI)0.54 (VIII)−0.54 (VII)0.69 (X)0.27 (IX)
Table
Table 2. Hydrogen bonding in the structure of IX. ΔE in kcal/mol.
Table 2. Hydrogen bonding in the structure of IX. ΔE in kcal/mol.
OH···A (ΔE)D···OH (ΔE)NH···A (ΔE)D···NH (ΔE)
IXaNHb (1.11)not specificNHc (3.18)OHb (1.11)
2.034 Å 2.439 Å2.034 Å
IXbNHa (1.67)not specificOHd (2.32)OHa (1.67)
2.193 Å 2.394 Å2.193 Å
IXcNHd (0.00)OHd (1.59)O1a (3.03)NHa (3.18)
2.076 Å2.216 Å2.123 Å2.439 Å
IXdOHc (1.59)NHb (2.32)O1b (2.17)OHc (0.00)
2.216 Å2.394 Å2.240 Å2.076
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Jakubec, M.; Císařová, I.; Karban, J.; Sýkora, J. The Effect of Deoxyfluorination on Intermolecular Interactions in the Crystal Structures of 1,6-Anhydro-2,3-epimino-hexopyranoses. Molecules 2022, 27, 278. https://doi.org/10.3390/molecules27010278

AMA Style

Jakubec M, Císařová I, Karban J, Sýkora J. The Effect of Deoxyfluorination on Intermolecular Interactions in the Crystal Structures of 1,6-Anhydro-2,3-epimino-hexopyranoses. Molecules. 2022; 27(1):278. https://doi.org/10.3390/molecules27010278

Chicago/Turabian Style

Jakubec, Martin, Ivana Císařová, Jindřich Karban, and Jan Sýkora. 2022. "The Effect of Deoxyfluorination on Intermolecular Interactions in the Crystal Structures of 1,6-Anhydro-2,3-epimino-hexopyranoses" Molecules 27, no. 1: 278. https://doi.org/10.3390/molecules27010278

APA Style

Jakubec, M., Císařová, I., Karban, J., & Sýkora, J. (2022). The Effect of Deoxyfluorination on Intermolecular Interactions in the Crystal Structures of 1,6-Anhydro-2,3-epimino-hexopyranoses. Molecules, 27(1), 278. https://doi.org/10.3390/molecules27010278

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